1887

Abstract

A study was carried out to clarify the taxonomy of four Gram-positive, heterotrophic mesophiles isolated from marine lakes in the Republic of Palau. The strains, designated YM3-251, YM3-653, YM3-671 and YM11-542, formed aerial and substrate mycelia. The cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid and alanine. The G+C content of their genomic DNA was approximately 45 mol%. The major fatty acid was iso-C and the major isoprenoid quinone was MK-9. The strains formed a distinct group in the 16S rRNA gene tree and shared a range of phenotypic properties that distinguished them from members of related genera in fam. nov. The name proposed to accommodate the new isolates is gen. nov., comprising two species based on genotypic and phenotypic criteria, including comparative and DNA–DNA relatedness data. The names proposed for these taxa are sp. nov., the type species, and sp. nov., with the type strains YM3-251 (=MBIC06230=DSM 44894) and YM11-542 (=MBIC06487=DSM 44955), respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64223-0
2006-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/12/2837.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64223-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Cole J. R., Chai B., Farris R. J., Wang Q., Kulam S. A., McGarrell D. M., Garrity G. M., Tiedje J. M. 2005; The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res 33: (Database Issue), D294–D296
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Harper J. J., Davis G. H. C. 1979; Two-dimensional thin-layer chromatography for amino acid analysis of bacterial cell walls. Int J Syst Bacteriol 29:56–58 [CrossRef]
    [Google Scholar]
  5. Hatayama K., Shoun H., Ueda Y., Nakamura A. 2005; Planifilum fimeticola gen. nov., sp. nov. and Planifilum fulgidum sp. nov., novel members of the family ‘ Thermoactinomycetaceae ’ isolated from compost. Int J Syst Evol Microbiol 55:2101–2104 [CrossRef]
    [Google Scholar]
  6. Kanoh K., Matsuo Y., Adachi K., Imagawa H., Nishizawa M., Shizuri Y. 2005; Mechercharmycins A and B, cytotoxic substances from marine-derived Thermoactinomyces sp. YM3-251. J Antibiot 58:289–292 [CrossRef]
    [Google Scholar]
  7. Katsuta A., Adachi K., Matsuda S., Shizuri Y., Kasai H. 2005; Ferrimonas marina sp. nov. Int J Syst Evol Microbiol 55:1851–1855 [CrossRef]
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  9. Lacey J., Cross T. 1989; Genus Thermoactinomyces Tsiklinsky 1899, 501AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp  2574–2585 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Nishijima M., Araki-Sakai M., Sano H. 1997; Identification of isoprenoid quinines by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28:113–122 [CrossRef]
    [Google Scholar]
  11. Nonomura H., Ohara Y. 1971; Distribution of actinomycetes in soil. X. New genus and species of monosporic actinomycetes in soil. J Ferment Technol 49:895–903
    [Google Scholar]
  12. Schleifer K. H., Kandler O. 1972; Peptidoglycan of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  13. Stackebrandt E., Woese C. R. 1981; Towards a phylogeny of the actinomycetes and related organisms. Curr Microbiol 5:197–202 [CrossRef]
    [Google Scholar]
  14. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  15. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882
    [Google Scholar]
  16. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  17. Yamamoto S., Harayama S. 1995; PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61:1104–1109
    [Google Scholar]
  18. Yoon J.-H., Park Y.-H. 2000; Phylogenetic analysis of the genus Thermoactinomyces based on 16S rDNA sequences. Int J Syst Evol Microbiol 50:1081–1086 [CrossRef]
    [Google Scholar]
  19. Yoon J.-H., Shin Y. K., Park Y.-H. 2000; DNA–DNA relatedness among Thermoactinomyces species: Thermoactinomyces candidus as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus as a synonym of Thermoactinomyces sacchari . Int J Syst Evol Microbiol 50:1905–1908
    [Google Scholar]
  20. Yoon J.-H., Kim I.-G., Shin Y.-K., Park Y.-H. 2005; Proposal of the genus Thermoactinomyces sensu stricto and three new genera, Laceyella , Thermoflavimicrobium and Seinonella , on the basis of phenotypic, phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 55:395–400 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64223-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64223-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error