1887

Abstract

A polyphasic study was undertaken to establish the taxonomic status of a Gram-positive, aerobic actinomycete, strain HPA177, isolated from a marine sponge, . The organism formed branching, non-fragmenting vegetative hyphae and produced black pigment. Chemotaxonomic characteristics were consistent with its assignment to the genus . Analysis of the 16S rRNA gene sequence showed that strain HPA177 formed a robust clade with type strains of the genus , but was distinct from them. A number of phenotypic characteristics also readily distinguished strain HPA177 from species of the genus with validly published names. On the basis of the above data, it is proposed that strain HPA177 represents a novel species, sp. nov. The type strain of is HPA177 (=CGMCC 4.2500=JCM 13436).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64217-0
2006-10-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/10/2309.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64217-0&mimeType=html&fmt=ahah

References

  1. Collins M. D. 1985; Isoprenoid quinone analyses in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp  267–284 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  2. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  3. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  4. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  5. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  6. Itoh T., Kudo T., Seino A. 1987; Chemotaxonomic studies on new genera of actinomycetes proposed in Chinese papers. Actinomycetologica 1:43–59 [CrossRef]
    [Google Scholar]
  7. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  9. Lechevalier M. P., Lechevalier H. A. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy , Special Publication 6 pp  277–284 Arlington, VA: Society for Industrial Microbiology;
    [Google Scholar]
  10. Lee Y. K., Kim H. W., Liu C. L., Lee H. K. 2003; A simple method for DNA extraction from marine bacteria that produce extracellular materials. J Microbiol Methods 52:245–250 [CrossRef]
    [Google Scholar]
  11. Liu Z., Zhang Y., Yan X. 1984; A new genus of the order Actinomycetales . Acta Microbiol Sin 24:295–298
    [Google Scholar]
  12. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  13. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  15. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note 101: Newark, DE: MIDI;
    [Google Scholar]
  16. Singla A. K., Mayilraj S., Kudo T., Krishnamurthi S., Prasad G. S., Vohra R. M. 2005; Actinoalloteichus spitiensis sp. nov., a novel actinobacterium isolated from a cold desert of the Indian Himalayas. Int J Syst Evol Microbiol 55:2561–2564 [CrossRef]
    [Google Scholar]
  17. Tamura T., Hatano K. 1998; Phylogenetic analyses on the strains belonging to invalidated genera of the order Actinomycetales . Actinomycetologica 12:15–28 [CrossRef]
    [Google Scholar]
  18. Tamura T., Liu Z., Zhang Y., Hatano K. 2000; Actinoalloteichus cyanogriseus gen. nov., sp. nov. Int J Syst Evol Microbiol 50:1435–1440 [CrossRef]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  20. Webster N. S., Hill R. T. 2001; The culturable microbial community of the Great Barrier Reef sponge Rhopaloeides odorabile is dominated by an α -proteobacterium. Mar Biol 138:843–851 [CrossRef]
    [Google Scholar]
  21. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989 Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [ English translation of Microbiology (Beijing)] 16176–178
  22. Yokota A., Tamura T., Hasegawa T., Huang L. H. 1993; Catenuloplanes japonicus gen. nov., sp. nov., nom. rev. a new genus of the order Actinomycetales . Int J Syst Bacteriol 43805–812 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64217-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64217-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error