RT Journal Article SR Electronic(1) A1 Kendall, Melissa M. A1 Liu, Yitai A1 Sieprawska-Lupa, Magdalena A1 Stetter, Karl O. A1 Whitman, William B. A1 Boone, David R.YR 2006 T1 Methanococcus aeolicus sp. nov., a mesophilic, methanogenic archaeon from shallow and deep marine sediments JF International Journal of Systematic and Evolutionary Microbiology, VO 56 IS 7 SP 1525 OP 1529 DO https://doi.org/10.1099/ijs.0.64216-0 PB Microbiology Society, SN 1466-5034, AB Three strains of CO2-reducing methanogens were isolated from marine sediments. Strain PL-15/HP was isolated from marine sediments of the Lipari Islands, near Sicily and the other two strains, Nankai-2 and Nankai-3T, were isolated from deep marine sediments of the Nankai Trough, about 50 km from the coast of Japan. Analysis of the cellular proteins and 16S rRNA gene sequences indicated that these three strains represented a single novel species that formed a deep branch of the mesophilic methanococci. Phylogenetic analysis indicated that the three strains were most closely related to Methanothermococcus okinawensis (95 % 16S rRNA gene sequence similarity). However, strains PL-15/HP, Nankai-2 and Nankai-3T grew at temperatures that were more similar to those of recognized species within the genus Methanococcus. Strain Nankai-3T grew fastest at 46 °C. Results of physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strains PL-15/HP, Nankai-2 and Nankai-3T from closely related species. The name Methanococcus aeolicus sp. nov. is proposed, with strain Nankai-3T (=OCM 812T=DSM 17508T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64216-0