1887

Abstract

A polyphasic study revealed taxonomic heterogeneity among reference strains of the species . Representative strains of and related taxa were investigated by partial sequence analysis of the housekeeping gene encoding the alpha-subunit of phenylalanyl-tRNA synthase (). Species-specific clusters were delineated for all taxa studied except for two strains, LMG 11494 and LMG 11984, respectively isolated from cheese and wheat, which occupied a distinct position. Their phylogenetic affiliation was determined using 16S rRNA gene sequence analysis and it was found that both strains (with 99.9 % gene sequence similarity between them) belonged to the group, with nearest neighbours and (gene sequence similarities of 99.2 and 98.1 %, respectively). Further genotypic and phenotypic studies, including fluorescent amplified fragment length polymorphism, DNA–DNA hybridization and DNA G+C content, clearly demonstrated that the two strains represent a single novel taxon for which the name sp. nov. is proposed (type strain LMG 11984=ATCC 53295).

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2006-07-01
2020-09-26
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