1887

Abstract

A polyphasic study revealed taxonomic heterogeneity among reference strains of the species . Representative strains of and related taxa were investigated by partial sequence analysis of the housekeeping gene encoding the alpha-subunit of phenylalanyl-tRNA synthase (). Species-specific clusters were delineated for all taxa studied except for two strains, LMG 11494 and LMG 11984, respectively isolated from cheese and wheat, which occupied a distinct position. Their phylogenetic affiliation was determined using 16S rRNA gene sequence analysis and it was found that both strains (with 99.9 % gene sequence similarity between them) belonged to the group, with nearest neighbours and (gene sequence similarities of 99.2 and 98.1 %, respectively). Further genotypic and phenotypic studies, including fluorescent amplified fragment length polymorphism, DNA–DNA hybridization and DNA G+C content, clearly demonstrated that the two strains represent a single novel taxon for which the name sp. nov. is proposed (type strain LMG 11984=ATCC 53295).

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2006-07-01
2019-10-15
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References

  1. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R. W., Kersters, K. & Vandamme, P. ( 1999; ). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  2. de Man, J. C., Rogosa, M. & Sharpe, M. E. ( 1960; ). A medium for the cultivation of lactobacilli. J Appl Bacteriol 23, 130–135.[CrossRef]
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Gancheva, A., Pot, B., Vanhonacker, K., Hoste, B. & Kersters, K. ( 1999; ). A polyphasic approach towards the identification of strains belonging to Lactobacillus acidophilus and related species. Syst Appl Microbiol 22, 573–585.[CrossRef]
    [Google Scholar]
  5. Gevers, D., Huys, G. & Swings, J. ( 2001; ). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205, 31–36.[CrossRef]
    [Google Scholar]
  6. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  7. Guarneri, T., Rossetti, L. & Giraffa, G. ( 2001; ). Rapid identification of Lactobacillus brevis using the polymerase chain reaction. Lett Appl Microbiol 33, 377–381.[CrossRef]
    [Google Scholar]
  8. Kandler, O. & Weiss, N. ( 1986; ). Genus Lactobacillus Beijerinck 1901, 212AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1209–1234. Edited by P. H. A. Sneath, N. S. Mair & J. G. Holt. Baltimore: Williams & Wilkins.
  9. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  10. Meroth, C. B., Hammes, W. P. & Hertel, C. ( 2004; ). Characterisation of the microbiota of rice sourdoughs and description of Lactobacillus spicheri sp. nov. Syst Appl Microbiol 27, 151–159.[CrossRef]
    [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  12. Naser, S. M., Thompson, F. L., Hoste, B., Gevers, D., Dawyndt, P., Vancanneyt, M. & Swings, J. ( 2005a; ). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151, 2141–2150.[CrossRef]
    [Google Scholar]
  13. Naser, S. M., Vancanneyt, M., De Graef, E. & 8 other authors ( 2005b; ). Enterococcus canintestini sp. nov., from faecal samples of healthy dogs. Int J Syst Evol Microbiol 55, 2177–2182.[CrossRef]
    [Google Scholar]
  14. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Schleifer, K. H. & Ludwig, W. ( 1995; ). Phylogeny of the genus Lactobacillus and related genera. Syst Appl Microbiol 18, 461–467.[CrossRef]
    [Google Scholar]
  17. Sohier, D., Coulon, J. & Lonvaud-Funel, A. ( 1999; ). Molecular identification of Lactobacillus hilgardii and genetic relatedness with Lactobacillus brevis. Int J Syst Bacteriol 49, 1075–1081.[CrossRef]
    [Google Scholar]
  18. Spiller, M. A. ( 1987; ). A mixture of a Lactobacillus brevis and Saccharomyces dairensis for preparing leavening barm. US Patent 4,666,719. 19 May 1987, US Patent Office.
  19. Stiles, M. E. & Holzapfel, W. H. ( 1997; ). Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol 36, 1–29.[CrossRef]
    [Google Scholar]
  20. Švec, P., Vancanneyt, M., Devriese, L. A., Naser, S. M., Snauwaert, C., Lefebvre, K., Hoste, B. & Swings, J. ( 2005a; ). Enterococcus aquimarinus sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55, 2183–2187.[CrossRef]
    [Google Scholar]
  21. Švec, P., Vancanneyt, M., Koort, J., Naser, S. M., Hoste, B., Vihavainen, E., Vandamme, P., Swings, J. & Björkroth, J. ( 2005b; ). Enterococcus devriesei sp. nov., associated with animal sources. Int J Syst Evol Microbiol 55, 2479–2484.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J. ( 2001; ). Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24, 520–538.[CrossRef]
    [Google Scholar]
  24. Torriani, S., Clementi, F., Vancanneyt, M., Hoste, B., Dellaglio, F. & Kersters, K. ( 2001; ). Differentiation of Lactobacillus plantarum, L. pentosus and L. paraplantarum species by RAPD-PCR and AFLP. Syst Appl Microbiol 24, 554–560.[CrossRef]
    [Google Scholar]
  25. Valcheva, R., Korakli, M., Onno, B., Prévost, H., Ivanova, I., Ehrmann, M. A., Dousset, X., Gänzle, M. G. & Vogel, R. F. ( 2005; ). Lactobacillus hammesii sp. nov., isolated from French sourdough. Int J Syst Evol Microbiol 55, 763–767.[CrossRef]
    [Google Scholar]
  26. Vancanneyt, M., Neysens, P., De Wachter, M. & 8 other authors ( 2005; ). Lactobacillus acidifarinae sp. nov. and Lactobacillus zymae sp. nov., from wheat sourdoughs. Int J Syst Evol Microbiol 55, 615–620.[CrossRef]
    [Google Scholar]
  27. Vogel, R. F., Bocker, G., Stolz, P. & 7 other authors ( 1994; ). Identification of lactobacilli from sourdough and description of Lactobacillus pontis sp. nov. Int J Syst Bacteriol 44, 223–229.[CrossRef]
    [Google Scholar]
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