1887

International Journal of Systematic and Evolutionary Microbiology

Volume 56, Issue 9

sp. nov., isolated from desert soil of Gansu Province in China Free

Abstract

A Gram-positive, endospore-forming, rod-shaped bacterium, designated strain B518, was isolated from a desert-soil sample from Gansu Province in China. The isolate was strictly aerobic and was motile by means of several flagella. The strain grew optimally at 35–40 °C and at pH 7.0–7.5. The predominant fatty acids of strain B518 were anteiso-C, C, anteiso-C, iso-C and iso-C. The G+C content of the genomic DNA was 50 mol% and the predominant quinone was MK-7. Comparative 16S rRNA gene sequence analyses showed that strain B518 formed a distinct phyletic line within the genus and was most closely related to IFO 15660, with a 16S rRNA gene sequence similarity of 95.8 %. The levels of 16S rRNA gene sequence similarity with respect to other type strains of species were below 95.1 %. On the basis of the chemotaxonomic data and molecular properties, strain B518 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is B518 (=KCTC 3950=DSM 16968).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64210-0
2006-09-01
2020-04-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/9/2131.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64210-0&mimeType=html&fmt=ahah

References

  1. Ash, C., Priest, F. G. & Collins, M. D. ( 1993; ). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie van Leeuwenhoek 64, 253–260.
     [Google Scholar]
  2. Bakermans, C. & Madsen, E. L. ( 2002; ). Diversity of 16S rDNA and naphthalene dioxygenase genes from coal-tar-waste-contaminated aquifer waters. Microb Ecol 44, 95–106.
     [Google Scholar]
  3. Daane, L. L., Harjono, I., Barns, S. M., Launen, L. A., Palleroni, N. J. & Haggblom, M. M. ( 2002; ). PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp. nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants. Int J Syst Evol Microbiol 52, 131–139.
     [Google Scholar]
  4. Enright, M. R., McInerney, J. O. & Griffin, C. T. ( 2003; ). Characterization of endospore-forming bacteria associated with entomopathogenic nematodes, Heterorhabditis spp., and description of Paenibacillus nematophilus sp. nov. Int J Syst Evol Microbiol 53, 435–441.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 2002; ). phylip (phylogeny inference package), version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  6. Gomori, G. ( 1955; ). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146.
     [Google Scholar]
  7. Jeon, C. O., Lee, D. S. & Park, J. M. ( 2003; ). Microbial communities in activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Water Res 37, 2195–2205.[CrossRef]
    [Google Scholar]
  8. Jeon, C. O., Lim, J. M., Lee, J. M., Xu, L. H., Jiang, C. L. & Kim, C. J. ( 2005; ). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55, 743–746.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–208.
     [Google Scholar]
  11. Kuroshima, K.-I., Sakane, T., Takata, R. & Yokota, A. ( 1996; ). Bacillus ehimensis sp. nov. and Bacillus chitinolyticus sp. nov., new chitinolytic members of the genus Bacillus. Int J Syst Bacteriol 46, 76–80.[CrossRef]
    [Google Scholar]
  12. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
     [Google Scholar]
  13. Lee, J.-S., Lee, K. C., Chang, Y.-H., Hong, S. G., Oh, H. W., Pyun, Y.-R. & Bae, K. S. ( 2002; ). Paenibacillus daejeonensis sp. nov., a novel alkaliphilic bacterium from soil. Int J Syst Evol Microbiol 52, 2107–2111.[CrossRef]
    [Google Scholar]
  14. Lee, J.-S., Pyun, Y.-R. & Bae, K. S. ( 2004; ). Transfer of Bacillus ehimensis and Bacillus chitinolyticus to the genus Paenibacillus with emended descriptions of Paenibacillus ehimensis comb. nov. and Paenibacillus chitinolyticus comb. nov. Int J Syst Evol Microbiol 54, 929–933.[CrossRef]
    [Google Scholar]
  15. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
     [Google Scholar]
  16. Lim, J.-M., Jeon, C. O., Lee, J.-C., Ju, Y. J., Park, D.-J. & Kim, C.-J. ( 2006; ). Bacillus koreensis sp. nov., a spore-forming bacterium, isolated from the vicinity of willow roots in Korea. Int J Syst Evol Microbiol 56, 59–63.[CrossRef]
    [Google Scholar]
  17. Montes, M. J., Mercade, E., Bozal, N. & Guinea, J. ( 2004; ). Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int J Syst Evol Microbiol 54, 1521–1526.[CrossRef]
    [Google Scholar]
  18. Rivas, R., Mateos, P. F., Martinez-Molina, E. & Velazquez, E. ( 2005a; ). Paenibacillus phyllosphaerae sp. nov., a xylanolytic bacterium isolated from the phyllosphere of Phoenix dactylifera. Int J Syst Evol Microbiol 55, 743–746.[CrossRef]
    [Google Scholar]
  19. Rivas, R., Gutierrez, C., Abril, A., Mateos, P. F., Martinez-Molina, E., Ventosa, A. & Velazquez, E. ( 2005b; ). Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum. Int J Syst Evol Microbiol 55, 1305–1309.[CrossRef]
    [Google Scholar]
  20. Saha, P., Mondal, A. K., Mayilraj, S., Krishnamurthi, S., Bhattacharya, A. & Chakrabarti, T. ( 2005; ). Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India. Int J Syst Evol Microbiol 55, 2577–2581.[CrossRef]
    [Google Scholar]
  21. Sánchez, M. M., Fritze, D., Blanco, A., Spröer, C., Tindall, B. J., Schumann, P., Kroppenstedt, R. M., Diaz, P. & Pastor, F. I. ( 2005; ). Paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the Ebro River delta. Int J Syst Evol Microbiol 55, 935–939.[CrossRef]
    [Google Scholar]
  22. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef]
    [Google Scholar]
  23. Šmerda, J., Sedláček, I., Páčová, Z., Durnová, E., Smíškova, A. & Havel, L. ( 2005; ). Paenibacillus mendelii sp. nov., from surface-sterilized seeds of Pisum sativum L. Int J Syst Evol Microbiol 55, 2351–2354.[CrossRef]
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  25. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt. Washington, DC: American Society for Microbiology.
  26. Takeda, M., Suzuki, I. & Koizumi, J. ( 2005; ). Paenibacillus hodogayensis sp. nov., capable of degrading the polysaccharide produced by Sphaerotilus natans. Int J Syst Evol Microbiol 55, 737–741.[CrossRef]
    [Google Scholar]
  27. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  28. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal_w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  29. Yoon, J. H., Kang, S. J., Yeo, S. H. & Oh, T. K. ( 2005; ). Paenibacillus alkaliterrae sp. nov., isolated from an alkaline soil in Korea. Int J Syst Evol Microbiol 55, 935–939.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64210-0
Loading
Paenibacillus gansuensis sp. nov., isolated from desert soil of Gansu Province in China
Int J Syst Evol Microbiol 56, 2131 (2006); https://doi.org/10.1099/ijs.0.64210-0
/content/journal/ijsem/10.1099/ijs.0.64210-0
/content/journal/ijsem/10.1099/ijs.0.64210-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2131-2134

Transmission electron micrographs showing the morphology, cell wall and spore structures of strain B518 , and a neighbour-joining phylogenetic tree for strain B518 and related taxa. [PDF](72 KB)

PDF

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error