1887

Abstract

Sequences of small-subunit rRNA genes have been obtained for four new isolates of . Phylogenetic analyses give new insights into the evolution of these organisms. A novel from pigs in Vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. Revival of the name for this organism is proposed. Instead of being related to , it shares a recent common ancestor with the non-encysting in a lineage that is basal to the tetranucleate cyst-producing clade. This suggests that species producing cysts with four nuclei are descended from an ancestor that produced cysts with a single nucleus. An from a horse was isolated in culture. No cysts were observed in the original stool sample but the sequence is placed unequivocally within the clade of tetranucleate cyst-producing species with no other sequences being specifically related. Revival of the name for this organism is proposed. The sequence was found to be the most closely related to and , as predicted, despite the organism having been an environmental isolate originally assigned to . Finally, a partial gene sequence from a pig proved to be virtually identical to that of from an ostrich, suggesting that the latter name is a synonym.

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2006-09-01
2019-10-19
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vol. , part 9, pp. 2235 - 2239

cysts from pigs in Vietnam. Cysts were concentrated using the formol-ether technique and subsequently stained with Lugol's iodine (A, B) or Trichrome (C, D) before photographing. The typical 'ring and dot' nucleus of species is visible in all cysts, while the cigar-shaped chromatoid bars are most clearly visible in (A) and (C). Bar, 10 µm. This is a colour version of Fig. 1 in the printed paper.



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