1887

Abstract

The aim of this study was to reinvestigate the relationships and the generic affiliations of the species , , and . The nicotinamide phosphoribosyltransferase gene () conferring V factor-independent growth was identified in . The gene encodes a polypeptide of 462 amino acids that shows 74.5 % amino acid sequence identity to the corresponding enzyme from . Ten isolates of all carried a pseudogene. DNA from was able to transform into the NAD-independent phenotype. The transformants carried a full-length inserted in the former locus of the pseudogene. The DNA–DNA relatedness between the type strains of and was 77 %. We conclude that the division into two species and is not justified and that should be considered a later heterotypic synonym of . Forty strains of , and were investigated by multilocus sequence analysis. The 40 strains form a monophyletic group clearly separate from other evolutionary lineages of the family . We propose the transfer of , and to a new genus gen. nov. as comb. nov. (the type species; type strain ATCC 33384=CCUG 13227=CIP 52.106=DSM 8324=NCTC 9710), comb. nov. (type strain ATCC 33389=CCUG 3715=CIP 70.73=NCTC 5906) and comb. nov. (type strain HK316=ATCC 33393=CCUG 10787=CCUG 12838=CIP 103292=NCTC 10977). The species of the genus are independent of X factor and variably dependent on V factor for growth .

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2006-09-01
2019-10-18
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vol. , part 9, pp. 2135 - 2146

Strains included in this study.

DNA-DNA relatedness between the type strain of [ ] and selected type strains of the .

Amplification by PCR of the gene in strains 12 [ ], 23 and 26 [ ] using primers 419r and 39r.

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