A novel thermophilic bacterium, strain KW1T, was isolated from a coastal hydrothermal field on the Satsuma Peninsula, Kagoshima Prefecture, Japan. The variably Gram-stained cells were motile rods with flagella, did not form spores and proliferated at 52–78 °C (optimum, 70 °C), pH 5–8 (optimum, pH 7) and 0–4.5 % NaCl (optimum, 1.0 %). The novel isolate was a strictly aerobic heterotroph that utilized complex proteinaceous substrates as well as a variety of carboxylic acids and amino acids. The G+C content of the genomic DNA was 70.8 mol%. Analysis of 16S rRNA gene sequences indicated that strain KW1T is closely related to Thermaerobacter subterraneus C21T (98.4 % sequence similarity). However, the DNA–DNA hybridization value for strain KW1T and T. subterraneus ATCC BAA-137T was below 46 %. On the basis of the molecular and physiological traits of strain KW1T, it represents a novel species of the genus Thermaerobacter, for which the name Thermaerobacter litoralis sp. nov. is proposed. The type strain is KW1T (=JCM 13210T=DSM 17372T).
AlfredssonG. A.,
KristjanssonJ. K.,
HjörleifsdottirS.,
StetterK. O.1979; Rhodothermus marinus , gen. nov., sp. nov, a thermophilic, halophilic bacterium from submarine hot springs in Iceland. J Gen Microbiol 134:49–68
BrillJ. A.,
WiegelJ.1997; Differentiation between spore-forming and asporogenic bacteria using a PCR and Southern hybridization based method. J Microbiol Methods 31:29–36[CrossRef]
BrownD. P.,
Genova-RaevaL.,
GreenB. D.,
WilkinsonS. R.,
YoungM.,
YoungmanP.1994; Characterization of spo0A homologues in diverse Bacillus and Clostridium species identifies DNA-binding domain. Mol Microbiol 14:411–426[CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229[CrossRef]
HokaiT.,
NishijimaM.,
MiyashitaH.,
MaruyamaT.1995; Dense community of hyperthermophilic sulfur-dependent heterotrophs in a geothermally heated shallow submarine biotope near Kodakara-Jima Island, Kagoshima, Japan. Appl Environ Microbiol 61:1931–1937
HuberR.,
WilharmT.,
HuberD.7 other authors1992; Aquifex pyrophilus gen. nov., sp. nov. represents a novel group of marine hyperthermophilic hydrogen-oxidizing bacteria. Syst Appl Microbiol 15:340–351[CrossRef]
LauererG.,
KristjanssonJ. K.,
LangworthyT. A.,
KönigH.,
StetterK. O.1986; Methanothermus sociabilis sp. nov., a second species within the Methanothermaceae growing at 97 °C. Syst Appl Microbiol 8:100–105[CrossRef]
OshimaT.,
ImahoriK.1974; Description of Thermus thermophilus (Yoshida and Oshima) comb. nov., a nonsporulating thermophilic bacterium from Japanese thermal spa. Int J Syst Bacteriol 24:102–112[CrossRef]
SakoY.,
NomuraN.,
UchidaA.,
IshidaY.,
MoriiH.,
KogaY.,
HoakiT.,
MaruyamaT.1996a; Aeropyrum pernix gen. nov., sp. nov. a novel aerobic hyperthermophilic archaeon growing at temperatures up to 100 °C. Int J Syst Bacteriol 46:1070–1077[CrossRef]
SpanevelloM. D.,
YamamotoH.,
PatelB. K. C.2002; Thermaerobacter subterraneus sp. nov., a novel aerobic bacterium from the Great Artesian Basin of Australia, and emendation of the genus Thermaerobacter
. Int J Syst Evol Microbiol 52:795–800[CrossRef]
TakaiK.,
InoueA.,
HorikoshiK.1999; Thermaerobacter marianensis gen. nov., sp. nov., an aerobic extremely thermophilic marine bacterium from the 11 000 m deep Mariana Trench. Int J Syst Bacteriol 49:619–628[CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]