1887

Abstract

A mesophilic bacterium, strain 15-52, was isolated from the rhizosphere of Chinese cabbage (). On the basis of phenotypic and genotypic characteristics, the bacterium was identified as representing a novel species belonging to the genus . The strain is non-flagellated, non-spore-forming and grows at temperatures in the range 1–37 °C. Physiological tests of the strain showed the presence of oxidase, catalase, protease (gelatin and casein hydrolysis), -glucosidase and -galactosidase activities. The highest levels of 16S rRNA gene sequence similarity were found with respect to CL-GP80 (97.3 %) and DS-27 (97.2 %). A phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain 15-52 is a member of the genus . DNA–DNA hybridization analysis revealed low levels of relatedness (<42.3 %) between the isolate and two phylogenetically related type strains, KCCM 42272 and KCTC 12559. The DNA G+C content is 44.2 mol% and the predominant fatty acids are iso-C (35.4 %), iso-C 2-OH and/or C 7 (27.8 %) and iso-C 3-OH (15.8 %). On the basis of these data, strain 15-52 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 15-52 (=KACC 11317=DSM 18130).

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2007-03-01
2024-04-25
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References

  1. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
    [Google Scholar]
  2. Gallego V., García M. T., Ventosa A. 2006; Pedobacter aquatilis sp. nov., a novel bacterium isolated from drinking water, and emended description of the genus Pedobacter . Int J Syst Evol Microbiol 56:1853–1858 [CrossRef]
    [Google Scholar]
  3. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  4. Hwang C. Y., Choi D. H., Cho B. C. 2006; Pedobacter roseus sp. nov., isolated from a hypertrophic pond. Int J Syst Evol Microbiol 56:1831–1836 [CrossRef]
    [Google Scholar]
  5. Joubert J. J., van Rensburg E. J., Pitout M. J. 1984; A plate method for demonstrating the breakdown of heparin and chondroitin sulphate by bacteria. J Microbiol Methods 2:197–202 [CrossRef]
    [Google Scholar]
  6. Kersters K., Hinz K.-H., Hertle A., Segers P., Lievens A., Siegmann O., De Ley J. 1984; Bordetella avium sp. nov., isolated from the respiratory tracts of turkeys and other birds. Int J Syst Bacteriol 34:56–70 [CrossRef]
    [Google Scholar]
  7. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  8. Kwon S. W., Kim J. S., Park I. C., Yoon S. H., Park D. H., Lim C. K., Go S. J. 2003; Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53:21–27 [CrossRef]
    [Google Scholar]
  9. Margesin R., Spröer C., Schumann P., Schinner F. 2003; Pedobacter cryoconitis sp. nov., a facultative psychrophile from alpine glacier cryoconite. Int J Syst Evol Microbiol 53:1291–1296 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  12. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa , Bacillus macerans , Bacillus azotofixans , and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154 [CrossRef]
    [Google Scholar]
  13. Shivaji S., Chaturvedi P., Reddy G. S. N., Suresh K. 2005; Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55:1083–1088 [CrossRef]
    [Google Scholar]
  14. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter , comprising four species: Pedobacter heparinus comb.nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov. Proposal of the family Sphingobacteriaceae fam. nov. Int J Syst Bacteriol 48:165–177 [CrossRef]
    [Google Scholar]
  16. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  17. Vanparys B., Heylen K., Lebbe L., De Vos P. 2005; Pedobacter caeni sp. nov., a novel species isolated from a nitrifying inoculum. Int J Syst Evol Microbiol 55:1315–1318 [CrossRef]
    [Google Scholar]
  18. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  19. Yoon J.-H., Lee M.-H., Kang S.-J., Park S.-Y., Oh T.-K. 2006; Pedobacter sandarakinus sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1273–1277 [CrossRef]
    [Google Scholar]
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