1887

Abstract

A novel obligate methylamine utilizer (strain JLW8), isolated from Lake Washington sediment, was characterized taxonomically. The isolate was an aerobic, Gram-negative bacterium. Cells were rod-shaped and motile by means of a single flagellum. Reproduction was by binary fission and no resting bodies were formed. Growth was observed within a pH range of 5–8.5, with optimum growth at pH 7.5. It utilized methylamine as a single source of energy, carbon and nitrogen. Methylamine was oxidized via methylamine dehydrogenase and formaldehyde was assimilated via the ribulose monophosphate cycle. The cellular fatty acid profile was dominated by C 7 and C and the major phospholipid was phosphatidylethanolamine. The DNA G+C content was 54 mol%. 16S rRNA gene sequence analysis indicated that the new isolate was closely related (97–98 % similarity) to a broad group of sequences from uncultured or uncharacterized , but only distantly related (93–96 % similarity) to known methylotrophs of the family . Strain JLW8 (=ATCC BAA-1282=DSM 17540) is proposed as the type strain of a novel species in a new genus within the family , gen. nov., sp. nov.

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2006-12-01
2019-10-17
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