1887

Abstract

Forty-five mycobacterial strains isolated from 23 Colombian HIV-positive patients were identified as members of the complex (MAC) and were characterized using different molecular approaches. Seven of the isolates showed characteristic features that allowed them to be differentiated from other members of the complex. The isolates had a novel 16S–23S rRNA internal transcribed spacer (ITS 1) gene sequence which is described as a new sequevar, MAC-X. All of the seven novel isolates gave a positive result with the MAC-specific AccuProbe (Gen-Probe), but tested negative for and species-specific probes (64 and 100 % of the isolates, respectively). The novel isolates could be differentiated phenotypically from other members of the MAC on the basis of the production of urease and by a consistent mycolic acid pattern. The novel isolates shared some characteristics with , such as the avium variant I (av-I) pattern of the 65 gene as determined by PCR restriction analysis and a positive PCR result for the (macrophage-induced) gene. However, the novel isolates showed a unique 16S rRNA gene sequence. DNA–DNA relatedness values, from 24 to 44 %, confirmed the distinction of the novel isolates from other members of the MAC at the genetic level and their status as members of a separate species. The novel isolates are proposed as representatives of a novel species, sp. nov., that is closely related to within the MAC. The type strain is 10B (=CIP 108962=CECT 3035).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64190-0
2006-09-01
2024-11-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/9/2049.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64190-0&mimeType=html&fmt=ahah

References

  1. Beggs M. L., Stevanova R., Eisenach K. D. 2000; Species identification of Mycobacterium avium complex isolates by a variety of molecular techniques. J Clin Microbiol 38:508–512
    [Google Scholar]
  2. CDC 1996 Standardized method for HPLC identification of Mycobacteria Atlanta: US Department of Health and Human Services;
    [Google Scholar]
  3. Clarridge J. E. III 2004; Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev 17:840–862 [CrossRef]
    [Google Scholar]
  4. Devulder G., Perouse de Montclos M., Flandrois J. P. 2005; A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. Int J Syst Evol Microbiol 55:293–302 [CrossRef]
    [Google Scholar]
  5. Guerrero C., Bernasconi C., Burki D., Bodmer T., Telenti A. 1995; A novel insertion element from Mycobacterium avium , IS1245, is a specific target for analysis of strain relatedness. J Clin Microbiol 33:304–307
    [Google Scholar]
  6. Jimenez M. S., Campos-Herrero M. I., Garcia D., Luquin M., Herrera L., Garcia M. J. 2004; Mycobacterium canariasense sp. nov. Int J Syst Evol Microbiol 54:1729–1734 [CrossRef]
    [Google Scholar]
  7. Keller A. P., Beggs M. L., Amthor B., Bruns F., Meissner P., Haas W. H. 2002; Evidence of the presence of IS1245 and IS1311 or closely related insertion elements in nontuberculous mycobacteria outside of the Mycobacterium avium complex. J Clin Microbiol 40:1869–1872 [CrossRef]
    [Google Scholar]
  8. Kent P., Kubica G. P. 1985 Public Health Mycobacteriology. A Guide for the Level III Atlanta: CDC, Department of Health and Human Services;
    [Google Scholar]
  9. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F. C., Bottger E. C. 1993; Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol 31:2882–2889
    [Google Scholar]
  10. Leao S. C., Bernardelli A., Cataldi A. 23 other authors 2005; Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean. J Microbiol Methods 61:193–199 [CrossRef]
    [Google Scholar]
  11. Lebrun L., Weill F. X., Lafendi L. 7 other authors; 2005; Use of the INNO-LiPA-MYCOBACTERIA assay (version 2) for identification of Mycobacterium avium–Mycobacterium intracellulare–Mycobacterium scrofulaceum complex isolates. J Clin Microbiol 43:2567–2574 [CrossRef]
    [Google Scholar]
  12. Linton C. J., Jalal H., Leeming J. P., Millar M. R. 1994; Rapid discrimination of Mycobacterium tuberculosis strains by random amplified polymorphic DNA analysis. J Clin Microbiol 32:2169–2174
    [Google Scholar]
  13. Mijs W., de Haas P., Rossau R., Van der Laan T., Rigouts L., Portaels F., van Soolingen D. 2002; Molecular evidence to support a proposal to reserve the designation Mycobacterium avium subsp. avium for bird-type isolates and ‘ M avium subsp. hominissuis ’ for the human/porcine type of M. avium . Int J Syst Evol Microbiol 521505–1518 [CrossRef]
    [Google Scholar]
  14. Morsczeck C., Berger S., Plum G. 2001; The macrophage-induced gene ( mig ) of Mycobacterium avium encodes a medium-chain acyl-coenzyme A synthetase. Biochim Biophys Acta 152159–65 [CrossRef]
    [Google Scholar]
  15. Novi C., Rindi L., Lari N., Garzelli C. 2000; Molecular typing of Mycobacterium avium isolates by sequencing of the 16S-23S rDNA internal transcribed spacer and comparison with IS1245-based fingerprinting. J Med Microbiol 49:1091–1095
    [Google Scholar]
  16. Pauls R. J., Turenne C. Y., Wolfe J. N., Kabani A. 2003; A high proportion of novel mycobacteria species identified by 16S rDNA analysis among slowly growing AccuProbe-negative strains in a clinical setting. Am J Clin Pathol 120:560–566 [CrossRef]
    [Google Scholar]
  17. Roiz M. P., Palenque E., Guerrero C., Garcia M. J. 1995; Use of restriction fragment length polymorphism as a genetic marker for typing Mycobacterium avium strains. J Clin Microbiol 33:1389–1391
    [Google Scholar]
  18. Smole S. C., McAleese F., Ngampasutadol J., Von Reyn C. F., Arbeit R. D. 2002; Clinical and epidemiological correlates of genotypes within the Mycobacterium avium complex defined by restriction and sequence analysis of hsp65 . J Clin Microbiol 40:3374–3380 [CrossRef]
    [Google Scholar]
  19. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  20. Telenti A., Marchesi F., Balz M., Bally F., Bottger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178
    [Google Scholar]
  21. Tortoli E. 2003; Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 16:319–354 [CrossRef]
    [Google Scholar]
  22. Tortoli E., Rindi L., Garcia M. J. 12 other authors 2004; Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov. Int J Syst Evol Microbiol 54:1277–1285 [CrossRef]
    [Google Scholar]
  23. Turenne C. Y., Semret M., Cousins D. V., Collins D. M., Behr M. A. 2006; Sequencing of hsp 65 distinguishes among subsets of Mycobacterium avium complex. J Clin Microbiol 44:433–440 [CrossRef]
    [Google Scholar]
  24. van Soolingen D., de Haas P. E., Hermans P. W., Groenen P. M., van Embden J. D. 1993; Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis . J Clin Microbiol 31:1987–1995
    [Google Scholar]
  25. van Soolingen D., Bauer J., Ritacco V. 8 other authors 1998; IS 1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization. J Clin Microbiol 36:3051–3054
    [Google Scholar]
  26. Welsh J., McClelland M. 1990; Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218 [CrossRef]
    [Google Scholar]
  27. Wong D. A., Yip P. C., Tse D. L., Tung V. W., Cheung D. T., Kam K. M. 2003; Routine use of a simple low-cost genotypic assay for the identification of mycobacteria in a high throughput laboratory. Diagn Microbiol Infect Dis 47:421–426 [CrossRef]
    [Google Scholar]
  28. Zeigler D. R. 2003; Gene sequences useful for predicting relatedness of whole genomes in bacteria. Int J Syst Evol Microbiol 53:1893–1900 [CrossRef]
    [Google Scholar]
  29. Zhang Y., Rajagopalan M., Brown B. A., Wallace R. J. Jr 1997; Randomly amplified polymorphic DNA PCR for comparison of Mycobacterium abscessus strains from nosocomial outbreaks. J Clin Microbiol 35:3132–3139
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64190-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64190-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error