1887

Abstract

Forty-five mycobacterial strains isolated from 23 Colombian HIV-positive patients were identified as members of the complex (MAC) and were characterized using different molecular approaches. Seven of the isolates showed characteristic features that allowed them to be differentiated from other members of the complex. The isolates had a novel 16S–23S rRNA internal transcribed spacer (ITS 1) gene sequence which is described as a new sequevar, MAC-X. All of the seven novel isolates gave a positive result with the MAC-specific AccuProbe (Gen-Probe), but tested negative for and species-specific probes (64 and 100 % of the isolates, respectively). The novel isolates could be differentiated phenotypically from other members of the MAC on the basis of the production of urease and by a consistent mycolic acid pattern. The novel isolates shared some characteristics with , such as the avium variant I (av-I) pattern of the 65 gene as determined by PCR restriction analysis and a positive PCR result for the (macrophage-induced) gene. However, the novel isolates showed a unique 16S rRNA gene sequence. DNA–DNA relatedness values, from 24 to 44 %, confirmed the distinction of the novel isolates from other members of the MAC at the genetic level and their status as members of a separate species. The novel isolates are proposed as representatives of a novel species, sp. nov., that is closely related to within the MAC. The type strain is 10B (=CIP 108962=CECT 3035).

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2006-09-01
2020-01-22
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Supplements

vol. , part 9, pp. 2049 - 2054

An alignment of the 65 gene sequences examined in this study.

RAPD patterns for the strains examined in this study.

[PDF file of Supplementary Figures S1 and S2](96 KB)



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