1887

Abstract

A novel actinomycete, strain WS-26, was isolated from beach sand on the coast of Jeju Island, Republic of Korea, and subjected to a polyphasic taxonomic characterization. The organism produced reddish orange-coloured substrate mycelium and white aerial mycelium, both of which fragmented into rod-shaped elements. A neighbour-joining tree, based on 16S rRNA gene sequence studies, revealed that the isolate formed a distinct branch at the base of a cluster that included , , , and . This branching pattern was also found in a tree constructed using the maximum-likelihood method, but was not supported by the maximum-parsimony method. Levels of 16S rRNA gene sequence similarity between the isolate and its phylogenetic neighbours ranged from 96.5 to 97.8 %. Chemotaxonomic properties, such as the principal amino acid of peptidoglycan, predominant menaquinone and polar lipids, supported its assignment to the genus . On the basis of phenotypic and phylogenetic data, the organism was different from species with validly published names. The name sp. nov. is proposed, with WS-26 (=KCCM 42317=NRRL B-24459) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64187-0
2006-09-01
2024-10-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/9/2203.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64187-0&mimeType=html&fmt=ahah

References

  1. Brosius J., Palmer J. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  2. Chun J., Seong C.-N., Bae K. S., Lee K.-J., Kang S.-O., Goodfellow M., Hah Y. C. 1998; Nocardia flavorosea sp. nov. Int J Syst Bacteriol 48:901–905 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Fitch W. M. 1971; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  7. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  8. Kageyama A., Poonwan N., Yazawa K., Mikami Y., Nishimura K. 2004a; Nocardia asiatica sp. nov., isolated from patients with nocardiosis in Japan and clinical specimens from Thailand. Int J Syst Evol Microbiol 54:125–130 [CrossRef]
    [Google Scholar]
  9. Kageyama A., Yazawa K., Nishimura K., Mikami Y. 2004b Nocardia testaceus sp. nov. and Nocardia senatus sp. nov., isolated from patients in Japan. Microbiol Immunol 48271–276 [CrossRef]
  10. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp  173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  11. Lechevalier H. A. 1989; Nocardioform actinomycetes. In Bergey's Manual of Systematic Bacteriology vol 4 pp  2348–2404 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Lechevalier M. P., Lechevalier H. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20:435–443 [CrossRef]
    [Google Scholar]
  13. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Ecol Syst 5:249–260 [CrossRef]
    [Google Scholar]
  14. Lee S. D. 2006; Nocardia jejuensis sp. nov., a novel actinomycete isolated from a natural cave on Jeju Island, Republic of Korea. Int J Syst Evol Microbiol 56:559–562 [CrossRef]
    [Google Scholar]
  15. Lee S. D., Kang S.-O., Hah Y. C. 2000a; Hongia gen. nov., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol 50:191–199 [CrossRef]
    [Google Scholar]
  16. Lee S. D., Kim E. S., Roe J.-H., Kim J.-H., Kang S.-O., Hah Y. C. 2000b; Saccharothrix violacea sp. nov., isolated from a gold mine cave, and Saccharothrix albidocapillata comb. nov. Int J Syst Evol Microbiol 50:1315–1323 [CrossRef]
    [Google Scholar]
  17. MacFaddin J. F. 1980 Biochemical Tests for Identification of Medical Bacteria , 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  19. Minnikin D. E. 1988; Isolation and purification of mycobacterial wall lipids. In Bacterial Cell Surface Techniques pp  125–135 Edited by Hancock I. C., Poxton I. R. Chichester: Wiley;
    [Google Scholar]
  20. Minnikin D. E., Alshamaony L., Goodfellow M. 1977; Differentiation of Mycobacterium , Nocardia , and related taxa by thin layer chromatographic analysis of whole-cell methanolysates. J Gen Microbiol 88:200–204
    [Google Scholar]
  21. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  22. Saddler G. S., Tavecchia P., Lociuro S., Zanol M., Colombo E., Selva E. 1991; Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14:185–191 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  25. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  26. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  28. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  29. Wang L., Zhang Y., Huang Y., Maldonado L. A., Liu Z., Goodfellow M. 2004; Nocardia pigrifrangens sp. nov., a novel actinomycete isolated from a contaminated agar plate. Int J Syst Evol Microbiol 54:1683–1686 [CrossRef]
    [Google Scholar]
  30. Yassin A. F., Rainey F. A., Mendrock U., Brzezinka H., Schaal K. P. 2000; Nocardia abscessus sp. nov. Int J Syst Evol Microbiol 50:1487–1493 [CrossRef]
    [Google Scholar]
  31. Yassin A. F., Rainey F. A., Steiner U. 2001; Nocardia cyriacigeorgici sp. nov. Int J Syst Evol Microbiol 51:1419–1423
    [Google Scholar]
  32. Yassin A. F., Sträubler B., Schumann P., Schaal K. P. 2003; Nocardia puris sp. nov. Int J Syst Evol Microbiol 53:1595–1599 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64187-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64187-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error