1887

Abstract

A non-motile, rod-shaped and pinkish-red-pigmented bacterium, designated strain WPCB131, was isolated from freshwater of Woopo wetland, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that this isolate represents a novel member of the genus with sequence similarities of 93.2–95.5 % to known species. No other taxa in the phylum showed more than 90 % sequence similarity to the isolate. Cells were Gram-negative, oxidase-negative and catalase-positive. Optimum growth was observed at pH 6 and 30 °C. The major fatty acids were iso-C (34.8 %), C 5 (15.0 %), anteiso-C B/iso-C I (14.4 %) and C 7/iso-C 2-OH (13.8 %). The DNA G+C content of strain WPCB131 was 65 mol%. The isolate could be readily distinguished from other species using a combination of phenotypic characters. On the basis of the polyphasic evidence presented in this study, it is proposed that strain WPCB131 (=IMSNU 14116=KCTC 12533=NBRC 101118) represents a novel species, sp. nov.

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2006-09-01
2024-04-18
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References

  1. Buczolits S., Denner E. B. M., Vybiral D., Wieser M., Kampfer P., Busse H. J. 2002; Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol 52:445–456
    [Google Scholar]
  2. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  3. Collins M. D., Hutson R. A., Grant I. R., Patterson M. F. 2000; Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov. Int J Syst Evol Microbiol 50:731–734 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  7. Hirsch P., Ludwig W., Hethke C., Sittig M., Hoffmann B., Gallikowski C. A. 1998; Hymenobacter roseosalivarius gen nov., sp. nov from continental Antarctic soils and sandstone: bacteria of the Cytophaga / Flavobacterium / Bacteroides line of phylogenetic descent. Syst Appl Microbiol 21374–383 [CrossRef]
    [Google Scholar]
  8. Jeon Y.-S., Chung H., Park S., Hur I., Lee J.-H., Chun J. 2005; jphydit: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [CrossRef]
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol  3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  10. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703
    [Google Scholar]
  11. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  14. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  15. Swofford D. L. 1998 paup – Phylogenetic Analysis Using Parsimony, version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  16. Yi H., Chun J. 2006; Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 44:171–176
    [Google Scholar]
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