1887

Abstract

A taxonomic study was performed on strain R2A10-2, isolated from greenhouse soil cultivated with lettuce ( L.), collected in Wanju Province, Korea. The bacterial cells were Gram-negative, aerobic, short rods. The growth temperature and pH were 5–35 °C and 5.0–9.0, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this isolate had 93.3–97.7 % similarity to species: the highest sequence similarities were to the type strains of (97.7 %), (97.1 %) and (96.9 %). Low levels of DNA–DNA relatedness were found between strain R2A10-2 and the type strains of these three species (<28 %). Differences in phenotypic properties were found with respect to species with validly published names. The predominant cellular fatty acids were iso-15 : 0 (40.0 %), iso-17 : 0 3-OH (21.9 %), iso-17 : 1ω9 (11.7 %) and summed feature 4 (iso-15 : 0 2-OH and/or 16 : 1ω7/, 11.0 %). Menaquinone MK-6 was detected as the sole respiratory quinone. The G+C content of the genomic DNA was 37.8 mol%. On the basis of the genomic and phenotypic evidence, this isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R2A10-2 (=KACC 11468=DSM 17724).

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2006-07-01
2019-09-16
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