1887

Abstract

A taxonomic study was performed on strain R2A10-2, isolated from greenhouse soil cultivated with lettuce ( L.), collected in Wanju Province, Korea. The bacterial cells were Gram-negative, aerobic, short rods. The growth temperature and pH were 5–35 °C and 5.0–9.0, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that this isolate had 93.3–97.7 % similarity to species: the highest sequence similarities were to the type strains of (97.7 %), (97.1 %) and (96.9 %). Low levels of DNA–DNA relatedness were found between strain R2A10-2 and the type strains of these three species (<28 %). Differences in phenotypic properties were found with respect to species with validly published names. The predominant cellular fatty acids were iso-15 : 0 (40.0 %), iso-17 : 0 3-OH (21.9 %), iso-17 : 1ω9 (11.7 %) and summed feature 4 (iso-15 : 0 2-OH and/or 16 : 1ω7/, 11.0 %). Menaquinone MK-6 was detected as the sole respiratory quinone. The G+C content of the genomic DNA was 37.8 mol%. On the basis of the genomic and phenotypic evidence, this isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R2A10-2 (=KACC 11468=DSM 17724).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64179-0
2006-07-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/7/1501.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64179-0&mimeType=html&fmt=ahah

References

  1. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  2. Breznak J. A., Costilow R. N. 1994; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology pp  137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. de Beer H., Hugo C. J., Jooste P. J., Willems A., Vancanneyt M., Coenye T., Vandamme P. A. R. 2005; Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant. Int J Syst Evol Microbiol 55:2149–2153 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  7. Holmes B., Owen R. J., McKeekin T. A. 1984a; Genus Flavobacterium Bergey, Harrison, Breed, Hammer and Hunton 1923, 97AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp  353–361 Edited by Krieg N. L., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. Holmes B., Owen R. J., Steigerwalt A. G., Brenner D. J. 1984b; Flavobacterium gleum , a new species found in human clinical specimens. Int J Syst Bacteriol 34:21–25 [CrossRef]
    [Google Scholar]
  9. Hugo C. J., Segers P., Hoste B., Vancanneyt M., Kersters K. 2003; Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53:771–777 [CrossRef]
    [Google Scholar]
  10. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
    [Google Scholar]
  11. Kim K. K., Bae H. S., Schumann P., Lee S. T. 2005a; Chryseobacterium daecheongense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 55:133–138 [CrossRef]
    [Google Scholar]
  12. Kim K. K., Kim M. K., Lim J. H., Park H. Y., Lee S. T. 2005b; Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55:1287–1293 [CrossRef]
    [Google Scholar]
  13. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001 mega2: molecular evolutionary genetics analysis software Tempe, AZ: Arizona State University;
    [Google Scholar]
  14. Kwon S. W., Kim J. S., Park I. C., Yoon S. H., Park D. H., Lim C. K., Go S. J. 2003; Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53:21–27 [CrossRef]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  16. Mudarris M., Austin B., Segers P., Vancanneyt M., Hoste B., Bernardet J. F. 1994; Flavobacterium scophthalmum sp. nov., a pathogen of turbot ( Scophthalmum maximus L.). Int J Syst Bacteriol 44:447–453 [CrossRef]
    [Google Scholar]
  17. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note no: 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  18. Seldin L., Dubnau D. 1985; Deoxyribonucleic acid homology among Bacillus polymyxa , Bacillus macerans , Bacillus azotofixans , and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154 [CrossRef]
    [Google Scholar]
  19. Shen F.-T., Kämpfer P., Young C.-C., Lai W.-A., Arun A. B. 2005; Chryseobacterium taichungense sp. nov., isolated from contaminated soil. Int J Syst Evol Microbiol 55:1301–1304 [CrossRef]
    [Google Scholar]
  20. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B. 1994; New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen.nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44:827–831 [CrossRef]
    [Google Scholar]
  21. Yabuuchi E., Kaneko T., Yano I., Moss C. W., Miyoshi N. 1983; Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb.nov., Sphingobacterium multivorum comb. nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov. glucose-nonfermenting gram-negative rods in CDC groups IIK-2 and IIb. Int J Syst Bacteriol 33:580–598 [CrossRef]
    [Google Scholar]
  22. Young C.-C., Kämpfer P., Shen F.-T., Lai W.-A., Arun A. B. 2005; Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64179-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64179-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error