A novel strain, R2A16-10, was isolated from greenhouse soil in Yeoju, Korea. The taxonomy of strain R2A16-10 was studied by using polyphasic methods. On the basis of 16S rRNA gene sequence analyses, strain R2A16-10 was found to be phylogenetically related to type strains of species (96.7–96.9 %), DSM 6220 (96.5 %), LMG 19981 (96.3 %) and species (94.9–95.7 %). Strain R2A16-10, which produced yellow-coloured colonies, was Gram-negative, rod-shaped (0.3–0.4×1.5–3.5 μm) and motile. The predominant fatty acids were 17 : 1 iso 9 (25.5 %), 15 : 0 iso (18.7 %) and 17 : 0 iso (14.6 %), and the major hydroxy fatty acids were 11 : 0 iso 3-OH (5.0 %), 13 : 0 iso 3-OH (3.4 %) and 17 : 0 iso 3-OH (1.0 %). The major isoprenoid quinone was Q-8. The G+C content of the DNA of the type strain was 63.0 mol%. On the basis of the data from this study, strain R2A16-10 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is R2A16-10 (=KACC 11405=DSM 17673).


Article metrics loading...

Loading full text...

Full text loading...



  1. An, D.-S., Im, W.-T., Yang, H.-C., Yang, D.-C. & Lee, S.-T. ( 2005; ). Dyella koreensis sp. nov., a β-glucosidase-producing bacterium. Int J Syst Evol Microbiol 55, 1625–1628.[CrossRef]
    [Google Scholar]
  2. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P. ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef]
    [Google Scholar]
  3. Breznak, J. A. & Costilow, R. N. ( 1994; ). Physicochemical factors in growth. In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  4. Entani, E., Ohmori, S., Masai, H. & Suzuki, K. ( 1985; ). Acetobacter polyoxogenes sp. nov., a new species of an acetic acid bacterium useful for producing vinegar with high acidity. J Gen Appl Microbiol 31, 475–490.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  8. Hiraishi, A. ( 1992; ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210–213.[CrossRef]
    [Google Scholar]
  9. Im, W.-T., Lee, S.-T. & Yokota, A. ( 2004; ). Rhodanobacter fulvus sp. nov., a β-galactosidase-producing gammaproteobacterium. J Gen Appl Microbiol 50, 143–147.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Tempe, AZ: Arizona State University.
  11. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  12. MacFaddin, J. F. ( 2000; ). Biochemical Tests for Identification of Medical Bacteria, 3rd edn, pp. 424–438. Baltimore: Lippincott Williams & Wilkins.
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Nalin, R., Simonet, P., Vogel, T. M. & Normand, P. ( 1999; ). Rhodanobacter lindaniclasticus gen. nov., sp. nov., a lindane-degrading bacterium. Int J Syst Bacteriol 49, 19–23.[CrossRef]
    [Google Scholar]
  15. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  16. Swings, J., De Ley, J. & Gillis, M. ( 1984; ). Genus III. Frateuria Swings, Kersters, De Vos, Gossele and De Ley, 1980, 547VP. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 210–213. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  17. Xie, C.-H. & Yokota, A. ( 2005; ). Dyella japonica gen. nov., sp. nov., a γ-proteobacterium isolated from soil. Int J Syst Evol Microbiol 55, 753–756.[CrossRef]
    [Google Scholar]

Data & Media loading...


vol. , part 9, pp. 2079 - 2082

Differential characteristics of strain R2A16-10 and the type strains of species and . [PDF](101 KB)


Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error