1887

Abstract

A sponge-associated strain, KMM 7019, was investigated in a polyphasic taxonomic study. The bacterium was strictly aerobic, heterotrophic, Gram-negative, yellow-pigmented, motile by gliding and oxidase-, catalase-, -galactosidase- and alkaline phosphatase-positive. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KMM 7019 is closely related to members of the genus , namely , and (97·7–98 % sequence similarities). The DNA–DNA relatedness between the strain studied and species ranged from 27 to 31 %, clearly demonstrating that KMM 7019 belongs to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KMM 7019 (=KCTC 12560=LMG 23205).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64167-0
2006-04-01
2020-10-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/4/883.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64167-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  3. Dobson S. J., Colwell R. R., McMeekin T. A., Franzmann P. D. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43:77–83 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, WA, USA:
    [Google Scholar]
  5. Kim S. B., Falconer C., Williams E., Goodfellow M. 1998; Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species isolated from soil. Int J Syst Bacteriol 48:59–68 [CrossRef]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  7. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  8. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  9. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp.nov. and Flavobacterium xanthum sp. nov., nom.rev., and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063 [CrossRef]
  10. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003a; Reichenbachia agariperforans gen. nov., sp. nov. a novel marine bacterium in the Cytophaga–Flavobacterium–Bacteroides phylum. Int J Syst Evol Microbiol 53:81–85 [CrossRef]
    [Google Scholar]
  11. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003b; Vitellibacter vladivostokensis gen. nov., sp. nov. a new member of the phylum Cytophaga–Flavobacterium–Bacteroides . Int J Syst Evol Microbiol 53:1281–1286 [CrossRef]
    [Google Scholar]
  12. Nedashkovskaya O. I., Kim S. B., Han S. K. 7 other authors 2003c; Mesonia algae gen nov., sp. nov a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm.. Int J Syst Evol Microbiol 531967–1971 [CrossRef]
    [Google Scholar]
  13. Nedashkovskaya O. I., Suzuki M., Vancanneyt M., Cleenwerck I., Zhukova N. V., Vysotskii M. V., Mikhailov V. V., Swings J. 2004; Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus . Int J Syst Evol Microbiol 54:1107–1110 [CrossRef]
    [Google Scholar]
  14. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Mikhailov V. V., Bae K. S., Kim I. S. 2005; Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 55:235–238 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64167-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64167-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error