1887

Abstract

The taxonomic position of three novel marine, heterotrophic, pigmented and agarolytic bacteria with gliding motility, isolated from the sea urchin , was investigated. 16S rRNA gene sequence analysis revealed that strains KMM 6166, KMM 6172 and KMM 6173 are members of the phylum ; their nearest neighbours were and (similarities of 94.5 and 93.6 %, respectively). The DNA G+C content of the strains was 44–45 mol%. The predominant fatty acids were C iso, C 5, C iso 9, C iso 3-OH and summed feature 3 (C 7 and/or C iso 2-OH). The major respiratory quinone was MK-7. Results of molecular experiments supported by phenotypic and chemotaxonomic data enabled the isolates to be classified as representatives of a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. is the type species of the genus , and its type strain is KMM 6172 (=KCTC 12368=LMG 23350).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64156-0
2006-05-01
2019-08-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/5/953.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64156-0&mimeType=html&fmt=ahah

References

  1. Bowman, J. P. ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868.
    [Google Scholar]
  2. Bowman, J. P., Nichols, C. M. & Gibson, J. A. E. ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef]
    [Google Scholar]
  3. Brettar, I., Christen, R. & Höfle, M. G. ( 2004a; ). Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 65–70.[CrossRef]
    [Google Scholar]
  4. Brettar, I., Christen, R. & Höfle, M. G. ( 2004b; ). Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 2335–2341.[CrossRef]
    [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 143–153.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1993; ). phylip (phylogenetic inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  7. Kim, S. B., Falconer, C., Williams, E. & Goodfellow, M. ( 1998; ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species isolated from soil. Int J Syst Bacteriol 48, 59–68.[CrossRef]
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Kluge, A. G. & Farris, F. S. ( 1969; ). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef]
    [Google Scholar]
  10. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  11. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4, 109–118.
    [Google Scholar]
  12. Nedashkovskaya, O. I., Kim, S. B., Han, S. K., Rhee, M. S., Lysenko, A. M., Falsen, E., Frolova, G. M., Mikhailov, V. V. & Bae, K. S. ( 2004a; ). Ulvibacter litoralis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 54, 119–123.[CrossRef]
    [Google Scholar]
  13. Nedashkovskaya, O. I., Vancanneyt, M., Van Trappen, S. & 7 other authors ( 2004b; ). Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004 . Int J Syst Evol Microbiol 54, 1757–1764.[CrossRef]
    [Google Scholar]
  14. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Mikhailov, V. V., Bae, K. S. & Kim, I. S. ( 2005a; ). Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55, 235–238.[CrossRef]
    [Google Scholar]
  15. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Frolova, G. M., Mikhailov, V. V., Bae, K. S., Lee, D. H. & Kim, I. S. ( 2005b; ). Gramella echinicola gen. nov., sp. nov., a novel halophilic bacterium of the family Flavobacteriaceae isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55, 391–394.[CrossRef]
    [Google Scholar]
  16. Nedashkovskaya, O. I., Vancanneyt, M., Dawyndt, P. & 9 other authors ( 2005c; ). Reclassification of [Cytophaga] marinoflava Reichenbach 1989 as Leeuwenhoekiella marinoflava gen. nov., comb. nov. and description of Leeuwenhoekiella aequorea sp. nov. Int J Syst Evol Microbiol 55, 1033–1038.[CrossRef]
    [Google Scholar]
  17. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Park, M. S., Mikhailov, V. V., Bae, K. S. & Park, H.-Y. ( 2005d; ). Roseivirga echinicomitans sp. nov., a novel marine bacterium isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Roseivirga. Int J Syst Evol Microbiol 55, 1797–1800.[CrossRef]
    [Google Scholar]
  18. Nedashkovskaya, O. I., Kim, S. B., Lee, M. S., Park, M. S., Lee, K. H., Lysenko, A. M., Oh, H. W., Mikhailov, V. V. & Bae, K. S. ( 2005e; ). Cyclobacterium amurskyense sp. nov., a novel marine bacterium isolated from sea water. Int J Syst Evol Microbiol 55, 2391–2394.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2004; ). Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 1969–1973.[CrossRef]
    [Google Scholar]
  21. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  22. Yi, H. & Chun, J. ( 2004; ). Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54, 157–162.[CrossRef]
    [Google Scholar]
  23. Yoon, J.-H., Yeo, S.-H. & Oh, T.-K. ( 2004; ). Hongiella marincola sp. nov., isolated from sea water of the East Sea in Korea. Int J Syst Evol Microbiol 54, 1845–1848.[CrossRef]
    [Google Scholar]
  24. Yoon, J.-H., Kang, S.-J., Jung, S.-Y., Lee, C.-H. & Oh, T.-K. ( 2005; ). Algoriphagus yeomjeoni sp. nov., isolated from a marine solar saltern in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 865–870.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64156-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64156-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error