gen. nov., sp. nov., isolated from muddy water, belonging to the family ‘Free

Abstract

A Gram-negative bacterial isolate (designated SRC-1) was isolated from an occasional drainage system and characterized by a polyphasic approach to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain SRC-1 with the family ‘’ of the phylum . It showed greatest sequence similarity to KMM 6156 (95.5 %) followed by MBRG1.5 (89.0 %) and DSM 11622 (88.9 %), but it differed from these micro-organisms in many phenotypic characteristics. Strain SRC-1 was an obligate aerobe and its cells were non-motile, irregular rods. The major fatty acids included mainly unsaturated and hydroxy fatty acids, including 17 : 1 iso I/anteiso B (36.7 %), 15 : 0 iso (15.8 %) and 17 : 0 iso 3-OH (10.3 %), and the DNA G+C content was 59.5 mol%. From the phenotypic and genotypic analyses it was clear that strain SRC-1 was quite different from members other genera in the family ‘’. Therefore we conclude that strain SRC-1 represents a novel genus, for which the name gen. nov., containing a single species sp. nov., is proposed. The type species of the genus is , the type strain of which is strain SRC-1 (=MTCC 7260=DSM 17521).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64144-0
2006-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/7/1703.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64144-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P., McCammon S. A., Brown M. V., Nichols D. S., McMeekin T. A. 1997; Diversity and association of psychrophilic bacteria in Antarctic sea ice. Appl Environ Microbiol 63:3068–3078
    [Google Scholar]
  2. Bowman J. P., Nichols C. M., Gibson J. A. E. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov., and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355 [CrossRef]
  3. Brettar I., Christen R., Hofle M. G. 2004; Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the Cytophaga–Flavobacterium–Bacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54:65–70 [CrossRef]
    [Google Scholar]
  4. Buczolits S., Denner E. B., Vybiral D., Wieser M., Kämpfer P., Busse H. J. 2002; Classification of three airborne bacteria and proposal of Hymenobacter aerophilus sp. nov. Int J Syst Evol Microbiol 52:445–456
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  6. Collins M. D., Huston R. A., Grant I. R., Patterson M. F. 2000; Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov. Int J Syst Evol Microbiol 50:731–734 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  8. Garrity G. M., Holt J. G. 2001; The road map to the Manual . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  119–166 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  9. Hirsch P., Ludwig W., Hethke C., Sittig M., Hoffmann B., Gallilowski C. A. 1998; Hymenobacter roseosalivarius gen. nov., sp. nov. from continental Antarctic soils and sandstone: bacteria of the Cytophaga/Flavobacterium/Bacteroides line of phylogenetic descent. Syst Appl Microbiol 21:374–383 [CrossRef]
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  11. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Ludwig W., Klenk H.-P. 2001; Overview: a phylogenetic backbone and taxonomic framework for prokaryotic systematics. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  49–65 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  13. Ludwig W., Strunk O., Klugbauer S., Klugbauer N., Weizenegger M., Neumaier J., Bachleitner M., Schleifer K. H. 1998; Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19:554–568 [CrossRef]
    [Google Scholar]
  14. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  15. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum Cytophaga–Flavobacterium–Bacteroides . Int J Syst Evol Microbiol 53:81–85 [CrossRef]
    [Google Scholar]
  16. Nedashkovskaya O. I., Kim S. B., Suzuki M. 9 other authors 2005; Pontibacter actiniarum gen. nov., sp. nov., a novel member of the phylum ‘ Bacteroidetes ’, and proposal of Reichenbachiella gen. nov. as a replacement for the illegitimate, prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. Int J Syst Evol Microbiol 55:2583–2588 [CrossRef]
    [Google Scholar]
  17. Pandey K. K., Mayilraj S., Chakrabarti T. 2002; Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52:1559–1567 [CrossRef]
    [Google Scholar]
  18. Rickard A. H., Stead A. T., O'May G. A., Lindsay S., Banner M., Handley P. S., Gilbert P. 2005; Adhaeribacter aquaticus gen. nov., sp. nov., a Gram-negative isolate from a potable water biofilm. Int J Syst Evol Microbiol 55:821–829 [CrossRef]
    [Google Scholar]
  19. Shivaji S., Rao N. S., Saisree L., Reddy G. S. N., Seshu Kumar G., Bhargava P. M. 1989; Isolates of Arthrobacter from the soils of Schirmacher Oasis, Antarctica. Polar Biol 10:225–229
    [Google Scholar]
  20. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  21. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  22. Suresh K., Reddy G. S. N., Sengupta S., Shivaji S. 2004a; Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54:457–461 [CrossRef]
    [Google Scholar]
  23. Suresh K., Prabagaran S. R., Sengupta S., Shivaji S. 2004b; Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. Int J Syst Evol Microbiol 54:1369–1375 [CrossRef]
    [Google Scholar]
  24. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36 [CrossRef]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  26. Van de Peer Y., De Wachter R. 1997; Construction of evolutionary distance trees with treecon for Windows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 13:227–230
    [Google Scholar]
  27. Yi H., Chun J. 2004; Hongiella mannitolivorans gen. nov., sp. nov., Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 54:157–162 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64144-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64144-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed