1887

Abstract

A buff-yellow-pigmented bacterium, strain K22-20, which was isolated from a cold desert of the Indian Himalayas, was subjected to a polyphasic taxonomic study. Phenotypic and chemical properties of strain K22-20 were consistent with its classification in the genus . The major fatty acids of the strain were iso-C 9 (-15-methyl 7-hexadecenoic acid), iso-C (13-methyl tetradecanoic acid), iso-C (14-methyl pentadecanoic acid) and iso-C (15-methyl hexadecanoic acid). The G+C content of the genomic DNA was 71 mol%. According to 16S rRNA gene sequence analysis, strain K22-20 was closely related to HKI 0124 (97.7 %). However, genomic relatedness between strain K22-20 and MTCC 6406, as revealed by DNA–DNA hybridization, was 64.5 %. Based on the polyphasic data, strain K22-20 (=MTCC 6545=DSM 17687=JCM 12763) represents a novel species of the genus , for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64138-0
2006-07-01
2020-11-30
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/7/1657.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64138-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. 1923 Bergey's Manual of Determinative Bacteriology Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Claus D., Berkeley R. C. W. 1986; Genus Bacillus Cohn 1872, 174AL . In Bergey's Manual of Systematic Bacteriology vol. 2 pp  1105–1139 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Collins M. D., Routh J., Saraswathy A., Lawson P. A., Schumann P., Welinder-Olsson C., Falsen E. 2004; Arsenicicoccus bolidensis gen. nov., sp. nov., a novel actinomycete isolated from contaminated lake sediment. Int J Syst Evol Microbiol 54:605–608 [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Groth I., Schumann P., Martin K., Schuetze B., Augsten K., Kramer I., Stackebrandt E. 1999; Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49:1717–1724 [CrossRef]
    [Google Scholar]
  8. Groth I., Schumann P., Weiss N., Schuetze B., Augsten K., Stackebrandt E. 2001; Ornithinimicrobium humiphilum gen. nov., sp. nov. a soil actinomycete with l-ornithine in the peptidoglycan. Int J Syst Evol Microbiol 51:81–87
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  10. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance–temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206
    [Google Scholar]
  11. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  12. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp  21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Orla-Jensen S. 1919 The Lactic Acid Bacteria Copenhagen: Høst;
    [Google Scholar]
  14. Pandey K. K., Mayilraj S., Chakrabarti T. 2002; Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52:1559–1567 [CrossRef]
    [Google Scholar]
  15. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  16. Reddy G. S. N., Prakash J. S. S., Prabahar V., Matsumoto G. I., Stackebrandt E., Shivaji S. 2003; Kocuria polaris sp. nov., an orange-pigmented psychrophilic bacterium isolated from an Antarctic cyanobacterial mat sample. Int J Syst Evol Microbiol 53:183–187 [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  18. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  19. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  20. Sheridan P. P., Loveland-Curtze J., Miteva I. V., Brenchley E. J. 2003; Rhodoglobus vestalii gen. nov., sp. nov. a novel psychrophilic organism isolated from an Antarctic Dry Valley lake. Int J Syst Evol Microbiol 53:985–994 [CrossRef]
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Smith N. R., Gordon R. E., Clark F. E. 1952; Aerobic spore-forming bacteria . US Department of Agriculture Agricultural Monograph no: 16 Washington, DC: US Department of Agriculture;
    [Google Scholar]
  23. Stackebrandt E., Rainey F. A., Ward-Rainey N. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  24. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  25. Takeuchi M., Hatano K. 1998; Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al . in a redefined genus Microbacterium . Int J Syst Bacteriol 48:739–747 [CrossRef]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  27. Tourova T. P., Antonov A. S. 1987; Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19:333–355
    [Google Scholar]
  28. Uchida K., Kudo T., Suzuki K. I., Nakase T. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56 [CrossRef]
    [Google Scholar]
  29. Von Wintzingerode F., Gobel U. B., Siddiqui R. A., Rosick U., Schumann P., Fruhling A., Rohde M., Pukall R., Stackebrandt E. 2001; Salana multivorans gen. nov., sp. nov., a novel actinobacterium isolated from an anaerobic bioreactor and capable of selenate reduction. Int J Syst Evol Microbiol 51:1653–1661 [CrossRef]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  31. Yamada K., Komagata K. 1972; Taxonomic studies on coryneform bacteria. V. Classification of coryneform bacteria. J Gen Appl Microbiol 18:417–431 [CrossRef]
    [Google Scholar]
  32. Yamamoto N., Sato S., Saito K., Hasuo T., Tadenuma M., Suzuki K., Tamaoka J., Komagata K. 1988; Rarobacter faecitabidus gen. nov., sp. nov., a yeast-lysing coryneform bacterium. Int J Syst Bacteriol 38:7–11 [CrossRef]
    [Google Scholar]
  33. Yi H., Schumann P., Sohn K., Chun J. 2004; Serinicoccus marinus gen. nov., sp. nov., a novel actinomycete with l-ornithine and l-serine in the peptidoglycan. Int J Syst Evol Microbiol 54:1585–1589 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64138-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64138-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error