1887

Abstract

Three Gram-negative, yellow-pigmented strains were isolated from the rhizospheres of plants grown in a compost-amended potting mix. The strains showed biological control activity towards the root-rot plant pathogen , and were characterized to determine their taxonomic position. Cells of the strains were non-motile rods, and the strains were oxidase- and catalase-positive and unable to ferment most sugars tested. The three strains showed differences in growth temperature range, optimal growth temperature and some biochemical reactions. The majority of the fatty acids were branched, and large amounts of 15 : 0 iso and 17 : 1 iso 9 were present. The 16S rRNA gene sequence (1497 bp) of strain B39 showed the highest level of similarity (98.5 %) to that of IAM 15025, followed by LMG 18385 (96.0 %; strain no longer extant), CCUG 50883 (96.4 %), DSM 16301 (96.3 %), LMG 1558 (96.2 %) and LMG 19981 (95.9 %). Less than 90 % 16S rRNA gene sequence similarity was observed for other members of the . The mean DNA–DNA reassociation value for the three strains was 100 % and was 25 % when the strains were compared with DNA from LMG 23003. The strains had a mean DNA G+C content of 67.6 mol%. On the basis of their phylogenetic, genomic and phenotypic properties, the three strains represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is strain B39 (=LMG 23181=DSM 17631).

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2006-08-01
2019-10-20
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References

  1. Coenye, T., Falsen, E., Vancanneyt, M., Hoste, B., Govan, J. R., Kersters, K. & Vandamme, P. ( 1999; ). Classification of Alcaligenes faecalis-like isolates from the environment and human clinical samples as Ralstonia gilardii sp. nov. Int J Syst Bacteriol 49, 405–413.[CrossRef]
    [Google Scholar]
  2. De Clercq, D., Vandesteene, L., Coosemans, J. & Ryckeboer, J. ( 2004; ). Use of compost as suppressor of plant diseases. In Resource Recovery and Reuse in Organic Solid Waste Management, pp. 317–337. Edited by P. Lens, B. Hamelers, H. A. J. Hoitink & W. Bidlingmaier. London: IWA Publishing.
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Goris, J., Suzuki, K., De Vos, P., Nakase, T. & Kersters, K. ( 1998; ). Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44, 1148–1153.[CrossRef]
    [Google Scholar]
  5. Hoitink, H. A. J. & Boehm, M. J. ( 1999; ). Biocontrol within the context of soil microbial communities: a substrate-dependent phenomenon. Annu Rev Phytopathol 37, 427–446.[CrossRef]
    [Google Scholar]
  6. Im, W.-T., Lee, S. T. & Yokota, A. ( 2004; ). Rhodanobacter fulvus sp. nov., a β-galactosidase-producing gammaproteobacterium. J Gen Appl Microbiol 50, 143–147.[CrossRef]
    [Google Scholar]
  7. MacFaddin, J. F. ( 1980; ). Biochemical Tests for the Identification of Medical Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  8. Mergaert, J., Verhelst, A., Cnockaert, M. C., Tan, T.-L. & Swings, J. ( 2001; ). Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences. Syst Appl Microbiol 24, 98–107.[CrossRef]
    [Google Scholar]
  9. Mergaert, J., Cnockaert, M. C. & Swings, J. ( 2002; ). Fulvimonas soli gen. nov., sp. nov., a γ-proteobacterium isolated from soil after enrichment on acetylated starch plastics. Int J Syst Evol Microbiol 52, 1285–1289.[CrossRef]
    [Google Scholar]
  10. Nalin, R., Simonet, P., Vogel, T. M. & Normand, P. ( 1999; ). Rhodanobacter lindaniclasticus gen. nov., sp. nov., a lindane-degrading bacterium. Int J Syst Bacteriol 49, 19–23.[CrossRef]
    [Google Scholar]
  11. Niemann, S., Puehler, A., Tichy, H.-V., Simon, R. & Selbitschka, W. ( 1997; ). Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population. J Appl Microbiol 82, 477–484.[CrossRef]
    [Google Scholar]
  12. Ryckeboer, J., Mergaert, J., Vaes, K., Klammer, S., De Clercq, D., Coosemans, J., Insam, H. & Swings, J. ( 2003; ). A survey of bacteria and fungi occurring during composting and self-heating processes. Ann Microbiol 53, 349–410.
    [Google Scholar]
  13. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software spackage for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  14. Van Trappen, S., Mergaert, J. & Swings, J. ( 2003; ). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 53, 1241–1245.[CrossRef]
    [Google Scholar]
  15. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2004; ). Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp. nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 85–92.[CrossRef]
    [Google Scholar]
  16. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  17. Wilson, K. ( 1987; ). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Greene Publishing and Wiley-Interscience.
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