1887

Abstract

A novel marine actinomycete, designated strain KST3-3, which was isolated from a sediment sample of the coast of Jeju, Korea, was subjected to a polyphasic taxonomic characterization. The organism was characterized morphologically by the formation of motile, coccoid cells. A phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the organism was related to the genera and , and members of the suborder , and occupied the deepest branch outside a taxon encompassing members of the genus . The organism showed relatively low levels of 16S rRNA gene sequence similarity to members of the genera (93.0–93.4 %) and (93.1–93.8 %). The morphological and chemotaxonomic characteristics, albeit with a slightly higher level of sequence similarity to members of the genus , supported its classification within the genus . On the basis of the polyphasic data presented, it was evident that the organism should be assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain KST3-3 (=KCCM 42250=NRRL B-24439).

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2006-06-01
2021-04-18
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References

  1. Brosius J., Palmer J. L., Kennedy J. P., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4480 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1993 phylip – Phylogeny Inference Package, version 3.51c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  4. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  5. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  6. Itoh T., Kudo T., Parenti F., Aeino A. 1989; Amended description of the genus Kineosporia , based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39:168–173 [CrossRef]
    [Google Scholar]
  7. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  8. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp  173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  9. Kudo T., Matushima K., Itoh T., Sasaki J., Suzuki K. 1998; Description of four new species of the genus Kineosporia : Kineosporia succinea sp.nov., Kineosporia rhizophila sp. nov., Kineosporiamikuniensis sp. nov., Kineosporia rhamnosa sp.nov., isolated from plant samples, and amended description of the genus Kineosporia . Int J Syst Bacteriol 48, 1245–1255 [CrossRef]
  10. Lechevalier M. P., Stern A. E., Lechevalier H. A. 1981; Phospholipids in the taxonomy of actinomycetes. Zentralbl Bakteriol Hyg Abt 1 Suppl 11:111–116
    [Google Scholar]
  11. Lee S. D., Kang S.-O., Hah Y. C. 2000; Hongia gen. nov., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol 50:191–199 [CrossRef]
    [Google Scholar]
  12. MacFaddin J. F. 1980 Biochemical Tests for Identification of Medical Bacteria , 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
    [Google Scholar]
  15. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  16. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  17. Phillips R. T., Wiegel J., Berry C. J., Filermans C., Peacock A. D., White D. C., Shimkets L. J. 2002; Kineococcus radiotolerans sp. nov., a radiation-resistant, Gram-positive bacterium. Int J Syst Evol Microbiol 52:933–938 [CrossRef]
    [Google Scholar]
  18. Saddler G. S., Tavecchia P., Lociuro S., Zanol M., Colombo E., Selva E. 1991; Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. J Microbiol Methods 14:185–191 [CrossRef]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  20. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  21. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  23. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  24. Yokota A., Tamura T., Nishii T., Hasegawa T. 1993; Kineococcus aurantiacus gen. nov., sp. nov., a new aerobic, gram-positive, motile coccus with meso -diaminopimelic acid and arabinogalactan in the cell wall. Int J Syst Bacteriol 43:52–57 [CrossRef]
    [Google Scholar]
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