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Abstract

A halotolerant bacterium was isolated from a saline lake located in Mallorca, Spain. Cells of the strain, designated MACL01, were Gram-negative, rod-shaped and motile by means of polar flagella. Colonies of strain MACL01 were white to cream in TSA medium, turning brown after 7 days of incubation; they were blue in thiosulphate/citrate/bile salts/sucrose agar medium. A neighbour-joining phylogenetic analysis based on 16S rRNA gene sequences showed that strain MACL01 belongs to the genus , in which it forms a distinct lineage together with and (showing 96.9 and 96.2 % similarity, respectively). The most closely related taxon according to phylogenetic analysis of the gene is also (90 % similarity). The gene also showed low similarity (83.7, 83.4 and 82.4 %, respectively) with respect to those of LMG 3772, LMG 4228 and LMG 22223. Neighbour-joining phylogenetic analysis of the and genes confirms that strain MACL01 belongs to the genus , forming a branch together with . Strain MACL01 was able to grow in 0–8 % NaCl. Growth occurred between 4 and 37 °C (optimum, 28 °C) and at pH 5–8.5. Luminescence was negative on marine agar. Strain MACL01 was found to be sensitive to the vibriostatic agent O/129. It reduced nitrate to nitrite, produced -galactosidase and hydrolysed gelatin, but did not produce arginine dihydrolase, indole or acetoin. Strain MACL01 used several carbohydrates and fermented glucose, -arabinose and sucrose. The most abundant fatty acids were summed feature 3 (32.6 %; comprising C 7 and/or C iso 2-OH), C (21.2 %) and C 7 (19.9 %). The G+C content of the genomic DNA was 49.8 mol%. On the basis of genotypic, phenotypic, chemotaxonomic and phylogenetic results, strain MACL01 (=LMG 22194=CECT 5860) should be classified as the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2006-05-01
2020-01-18
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Electron micrograph of strain MACL01 grown in nutrient agar medium for 48 h and stained with uranyl acetate. The cells are rod-shaped and flagella are visible. Bar, 0.57 µm.

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[PDF file of Supplementary Figs S2 and S3](27 KB)

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Differential phenotypic characteristics of sp. nov. MACL01 and related species. [PDF](35 KB)

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