1887

Abstract

Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to L1-82 (DSM 14610), were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5×1.5–5.0 μm and were motile. Two strains belonging to one cluster (A2-181 and A2-183) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1 gave weak growth). All strains were net acetate utilizers. The DNA G+C contents of representative strains A2-183, A2-194, M72/1 and L1-82 were 47.4, 41.4, 42.0 and 42.6 mol%, respectively. Based on 16S rRNA gene sequence similarity, three novel species are proposed, with the names sp. nov. (type strain A2-183=DSM 16839=NCIMB 14029), sp. nov. (type strain A2-194=DSM 16841=NCIMB 14030) and sp. nov. (type strain M72/1=DSM 16840=NCIMB 14031).

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2006-10-01
2021-10-20
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