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Abstract

A Gram-negative, rod-shaped, non-spore-forming betaproteobacterium (TRO-001DR8) was isolated from humic-lake samples collected from northern Wisconsin, USA. On the basis of 16S rRNA gene sequence analysis, strain TRO-001DR8 belonged to the family , and the phylogenetic distance from its closest relative, , was 95 %. Strain TRO-001DR8 lacked the violet pigmentation of and shared only 26 % DNA–DNA relatedness with . The DNA G+C content of strain TRO-001DR8 was 59 mol%. The predominant fatty acids were C 7 + C 7 2-OH iso (52·5 %), C (21·7 %), C 7 (8·0 %) and C (5·1 %). Strain TRO-001DR8 grew optimally at 35 °C and pH 6·0, did not utilize sucrose, but did use glucose, some organic acids and most protein amino acids. Biochemical, physiological, chemotaxonomic and phylogenetic analyses showed that strain TRO-001DR8 could not be assigned to any known genus of the . Therefore, the isolate represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is TRO-001DR8 (=ATCC BAA-1216=BCCM/LMG 23054).

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2006-04-01
2021-02-25
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