1887

Abstract

An aquatic bacterium, strain GPTSA100-15, was isolated on nutritionally poor medium TSBA100 (tryptic soy broth diluted 100 times and solidified with 1.5 % agarose) and characterized using a polyphasic approach. The isolate was unable to grow on commonly used nutritionally rich media such as tryptic soy agar, nutrient agar and Luria–Bertani agar. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was affiliated with the family ‘’ in the phylum . Phylogenetically, it showed closest similarity (94.0 %) with an uncultured bacterial clone, HP1A92, detected in a sludge microbial community. Among the culturable bacteria, the isolate had highest 16S rRNA gene sequence similarity with 4M15 (87.8 %). Sequence similarities with other members of the phylum were less than 85 %. The fatty acid profile of the isolate grown on TSBA100 indicated that the major fatty acid was iso-C, which is also present in many members of the family ‘’. Cells of strain GPTSA100-15 are Gram-negative, strictly aerobic rods. The DNA G+C content of the isolate is 36.9 mol%. Results of phenotypic, chemotaxonomic and phylogenetic analyses clearly indicate that strain GPTSA100-15 represents a new genus within the family ‘’; the name gen. nov. is proposed for the genus, with sp. nov. as the type species. The type strain of is GPTSA100-15 (=MTCC 6937=DSM 17448).

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2006-05-01
2019-10-20
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vol. , part 5, pp. 991 - 995

Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequence analysis showing the relative position of strain GPTSA100-15 among various representative members of the phylum . [PDF](24 KB)



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