1887

Abstract

Two novel spore-forming, Gram-positive, mesophilic, heterotrophic bacteria representing two novel species were isolated from the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) at Pasadena, CA, USA. The incidence of similar strains was examined by screening the growing collection of isolates (∼400 strains) obtained from the JPL-SAF using species-specific PCR primer sets designed from the 16S rRNA gene sequences of strains SAFN-016 and SAFN-007. Phylogenetic analysis of 16S rRNA gene sequences placed these novel isolates within the genus . Two strains, SAFN-016 and SAFN-125, shared 98 % 16S rRNA gene sequence similarity with and 97 % similarity with . Strain SAFN-007 showed 95.2 % 16S rRNA gene sequence similarity with , its nearest phylogenetic neighbour. The results of DNA–DNA hybridization, physiological tests and biochemical analysis allowed genotypic and phenotypic differentiation of the isolates from currently recognized species. Strain SAFN-007 and strains SAFN-016 and SAFN-125 are representatives of two separate novel species, for which the names sp. nov. (type strain SAFN-007=ATCC BAA-1211=NBRC 101214) and sp. nov. (type strain SAFN-016=ATCC BAA-1209=NBRC 101215) are proposed.

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2006-07-01
2019-10-19
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