RT Journal Article SR Electronic(1) A1 Im, Wan-Taek A1 Jung, Hae-Min A1 Ten, Leonid N. A1 Kim, Myung Kyum A1 Bora, Nagamani A1 Goodfellow, Michael A1 Lim, Sangyong A1 Jung, Jinwoo A1 Lee, Sung-TaikYR 2008 T1 Deinococcus aquaticus sp. nov., isolated from fresh water, and Deinococcus caeni sp. nov., isolated from activated sludge JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 10 SP 2348 OP 2353 DO https://doi.org/10.1099/ijs.0.64082-0 PB Microbiology Society, SN 1466-5034, AB The taxonomic positions of two environmental isolates from South Korea were established using a combination of genotypic and phenotypic data. The organisms, designated PB314T and Ho-08T, were Gram-negative, rod-shaped and non-spore-forming and had chemotaxonomic properties consistent with their classification in the genus Deinococcus 16S rRNA gene tree, the highest sequence similarities being shown to the type strains of Deinococcus grandis (96.3–96.7 %) and Deinococcus indicus (96.3–96.4 %). The isolates shared relatively high 16S rRNA gene sequence similarity (98.1 %) but had a DNA–DNA relatedness value of only 22 %. Chemotaxonomic data revealed that both strains possess quinone system MK-8 as the predominant compound, C16 : 1 ω7c and C16 : 0 as major fatty acids and ornithine as a diamino acid in the peptidoglycan structure, corroborating our assignment of the strains to the genus Deinococcus. The results of phylogenetic analyses based on 16S rRNA gene sequences, DNA–DNA relatedness values and physiological and biochemical tests clearly demonstrated that the two strains represent distinct species. On the basis of these data, two novel species, Deinococcus aquaticus sp. nov. (type strain PB314T =KCTC 12552T =NBRC 101311T) and Deinococcus caeni sp. nov. (type strain Ho-08T =KCTC 12553T =NBRC 101312T), are proposed., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64082-0