1887

Abstract

A phenanthrene-degrading bacterium, strain TKP, was isolated from a fly ash dumping site of the thermal power plant in Panki, Kanpur, India, by an enrichment culture method using phenanthrene as the sole source of carbon and energy. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus , as it showed highest sequence similarity to DSM 11019 (97.3 %) and JCM 10874 (96.5 %), compared with only 91–93 % similarity to members of other genera such as , , and . In DNA–DNA hybridization experiments with strains that were closely related phylogenetically and in terms of 16S rRNA gene sequences, i.e. DSM 11019 and JCM 10874, strain TKP showed less than 70 % relatedness. Strain TKP contained sphingoglycolipids SGL-1 and SGL-2 and 18 : 17 as the predominant fatty acid, with 16 : 0 as a minor component and 14 : 0 2-OH as the major 2-hydroxy fatty acid. Thus, phylogenetic analysis, DNA–DNA hybridization, fatty acid and polar lipid profiles and differences in physiological and morphological features from the most closely related members of the group showed that strain TKP represents a distinct species of . The name sp. nov. is proposed, with the type strain TKP (=MTCC 7295=CCM 7327). JCM 10874 formed a coherent cluster with members of , did not reduce nitrate to nitrite and had a fatty acid profile similar to those of species; hence should be transferred to the genus as comb. nov., with the type strain JCM 10874 (=DSM 14926).

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2006-09-01
2019-10-17
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Two-dimensional thin-layer chromatography of polar lipids of strain TKP . PE, Phosphatidylethanolamine; PG, phosphatidylglycerol; DPG, diphosphatidylglycerol; PC, phosphatidylcholine; SGL1 and SGL2, sphingoglycolipids; PN, aminophospholipid; PL, unidentified phospholipid; GL1, unidentified glycolipid (circled).

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