1887

Abstract

Two yeast strains, 153M and NCYC 3133, isolated from clinical sources in separate hospitals were found to be almost identical in the sequences of the D1/D2 domain of large-subunit rDNA, the PCR fingerprinting profiles and physiological characteristics. The isolates are phenotypically similar, although not identical, to and (recently renamed ). Sequence analysis of the 26S rDNA D1/D2 gene variable region revealed that the two clinical isolates were closely related phylogenetically to and , but differed sufficiently to justify their assignment as representatives of a separate species. The name sp. nov. is proposed for the novel species with the type strain 153M (=CBS 10154=NCYC D3853=CECT 12000).

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2006-01-01
2020-05-31
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References

  1. Ahearn, D. G. ( 1998; ). Yeasts pathogenic for humans. In The Yeasts: a Taxonomic Study, 4th edn, pp. 9–12. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  2. Barnett, J. A., Payne, R. W. & Yarrow, D. ( 2000; ). Yeasts: Characteristics and Identification, 3rd edn. Cambridge: Cambridge University Press.
  3. Correia, A., Sampaio, P., Almeida, J. & Pais, C. ( 2004; ). Study of molecular epidemiology of candidiasis in Portugal by PCR fingerprinting of Candida clinical isolates. J Clin Microbiol 42, 5899–5903.[CrossRef]
    [Google Scholar]
  4. Fidel, P. L., Jr, Vazquez, J. A. & Sobel, J. D. ( 1999; ). Candida glabrata: review of epidemiology, pathogenesis, and clinical disease with comparison to C. albicans. Clin Microbiol Rev 12, 80–96.
    [Google Scholar]
  5. James, S. A., Bond, C. J., Stratford, M. & Roberts, I. N. ( 2005; ). Molecular evidence for the existence of natural hybrids in the genus Zygosaccharomyces. FEMS Yeast Res 5, 747–755.[CrossRef]
    [Google Scholar]
  6. Kurtzman, C. P. ( 2003; ). Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. FEMS Yeast Res 4, 233–245.[CrossRef]
    [Google Scholar]
  7. Kurtzman, C. P. & Robnett, C. J. ( 1997; ). Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5′ end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol 35, 1216–1223.
    [Google Scholar]
  8. Kurtzman, C. P. & Robnett, C. J. ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef]
    [Google Scholar]
  9. Lachance, M. A. ( 1998; ). Kluyveromyces van der Walt emend. Van der Walt. In The Yeasts: a Taxonomic Study, 4th edn, pp. 227–247. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  10. Meyer, S. A., Payne, R. W. & Yarrow, D. ( 1998; ). Candida Berkhout. In The Yeasts: a Taxonomic Study, 4th edn, pp. 454–573. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  11. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  12. Sampaio, J. P., Gadanho, M., Santos, S., Duarte, F. L., Pais, C., Fonseca, A. & Fell, J. W. ( 2001; ). Polyphasic taxonomy of the basidiomycetous yeast genus Rhodosporidium: Rhodosporidium kratochvilovae and related anamorphic species. Int J Syst Evol Microbiol 51, 687–697.
    [Google Scholar]
  13. Sullivan, D. J., Westerneng, T. J., Haynes, K. A., Bennett, D. E. & Coleman, D. C. ( 1995; ). Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals. Microbiology 141, 1507–1521.[CrossRef]
    [Google Scholar]
  14. Swofford, D. L. ( 2000; ). paup*: Phylogenetic analysis using parsimony (* and other methods). Sunderland, MA: Sinauer Associates.
  15. Tavanti, A., Davidson, A. D., Gow, N. A. R., Maiden, M. C. J. & Odds, F. C. ( 2005; ). Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III. J Clin Microbiol 43, 284–292.[CrossRef]
    [Google Scholar]
  16. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  17. White, T. J., Bruns, T., Lee, S. & Taylor, J. ( 1990; ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide for Methods and Applications, pp. 315–322. Edited by M. A. Innis, D. H. Gelfand, J. J. Sninsky & T. J. Taylor. New York: Academic Press.
  18. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance, classification and identification of yeasts. In The Yeasts: a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
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vol. , part 1, pp. 313 - 317

Pairwise alignment of partial sequences of 26S rDNA D1/D2 region of , and strain 153M . [PDF](918 KB)



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