1887

Abstract

A Gram-negative, almost straight, rod-shaped, non-motile bacterium, designated CL-GP79, was isolated from a highly eutrophic pond located within the campus of Seoul National University, Korea. Phylogenetic analyses based on 16S rRNA gene sequencing showed that strain CL-GP79 belongs to the family , with as its closest relative (similarity of 95.7 %). The cellular fatty acids consist mainly of C 5 (mean±, 26.9±10.8 %), iso-C 2-OH and/or C 7 (19.2±2.3 %) and iso-C (12.1±1.3 %). The DNA G+C content was found to be 38.3 mol%. Phenotypic, chemotaxonomic and phylogenetic analyses indicated that strain CL-GP79 could be assigned to the genus , but could be distinguished from . The strain CL-GP79 therefore represents a novel species, for which the name sp. nov. is proposed, with CL-GP79 (=KCCM 42271=JCM 13398) as the type strain.

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2006-06-01
2020-09-24
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496
    [Google Scholar]
  3. Borrall R., Larkin J. M. 1978; Flectobacillus marinus (Raj) comb. nov., a marine bacterium previously assigned to Microcyclus . Int J Syst Bacteriol 28:341–343 [CrossRef]
    [Google Scholar]
  4. Cole J. R., Chai B., Marsh T. L. 8 other authors 2003; The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31:442–443 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  7. Gosink J. J., Woese C. R., Staley J. T. 1998; Polaribacter gen. nov., with three new species, P.irgensii sp. nov., P. franzmannii sp.nov. and P. filamentus sp. nov.,gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘ Flectobacillus glomeratus ’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48:223–235 [CrossRef]
    [Google Scholar]
  8. Gromov B. V. 1963; A new bacterium of the genus Microcyclus . Dokl Akad Nauk SSSR 152:733–734 (in Russian
    [Google Scholar]
  9. Hansen G. H., Sørheim R. 1991; Improved method for phenotypical characterization of marine bacteria. J Microbiol Methods 13:231–241 [CrossRef]
    [Google Scholar]
  10. Jeon Y. S., Chung H., Park S., Hur I., Lee J. H., Chun J. 2005; jphydit: a java-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [CrossRef]
    [Google Scholar]
  11. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  12. Kumar S., Tamura K., Nei M. 2004; mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  13. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  14. Larkin J. M., Borrall R. 1984; Genus III. Flectobacillus Larkin, Williams and Taylor 1977, 152AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp  129–132 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  15. Larkin J. M., Williams P. M., Taylor R. 1977; Taxonomy of the genus Microcyclus Ørskov 1928: reintroduction and emendation of the genus Spirosoma Migula 1894 and proposal of a new genus, Flectobacillus . Int J Syst Bacteriol 27:147–156 [CrossRef]
    [Google Scholar]
  16. McGuire A. J., Franzmann P. D., McMeekin T. A. 1987; Flectobacillus glomeratus sp. nov., a curved, nonmotile, pigmented bacterium isolated from antarctic marine environments. Syst Appl Microbiol 9:265–272 [CrossRef]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  18. Nikitin D. I., Oranskaya M. S., Pitryuk I. A., Chernykh N. A., Lysenko A. M. 1994; A new ring-forming bacterium Arcocella aquatica gen. et sp. nov. Mikrobiologiia 63:152–159 (in Russian
    [Google Scholar]
  19. Nikitin D. I., Strömpl C., Oranskaya M. S., Abraham W.-R. 2004; Phylogeny of the ring-forming bacterium Arcicella aquatica gen. nov., sp. nov. ( ex Nikitin et al . 1994), from a freshwater neuston biofilm. Int J Syst Evol Microbiol 54:681–684 [CrossRef]
    [Google Scholar]
  20. Posada D., Crandall K. A. 1998; modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818 [CrossRef]
    [Google Scholar]
  21. Raj H. D., Maloy S. R. 1990; Proposal of Cyclobacterium marinus gen. nov., comb. nov., for a marine bacterium previously assigned to the genus Flectobacillus . Int J Syst Bacteriol 40:337–347 [CrossRef]
    [Google Scholar]
  22. Rosselló-Mora R., Amann R. 2001; The species concept for prokaryotes. FEMS Microbiol Rev 25:39–67 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  26. Swofford D. L. 1998 paup* – Phylogenetic Analysis Using Parsimony, version 4 Sunderland, MA: Sinauer;
    [Google Scholar]
  27. Urakami T., Komagata K. 1986; Methanol-utilizing Ancylobacter strains and comparison of their cellular fatty acid compositions and quinone systems with those of Spirosoma , Flectobacillus , and Runella species. Int J Syst Bacteriol 36:415–421 [CrossRef]
    [Google Scholar]
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