1887

Abstract

A Gram-negative, chemoheterotrophic, facultatively anaerobic, slightly halophilic, oval-shaped marine bacterium, designated HTCC2601, was isolated from the western Sargasso Sea by high-throughput culturing involving dilution to extinction. Although the 16S rRNA gene sequence similarity between the isolate and was 96·5 %, phylogenetic analyses using different treeing algorithms clearly indicated that the strain forms a distinct lineage within a clade containing the recently classified genera and in the order of the . The DNA–DNA relatedness between strain HTCC2601 and was 26·3 %. Strain HTCC2601 utilized a wide range of carbohydrates, including hexose monomers, sugar alcohols, organic acids and amino acids, as sole carbon sources. The DNA G+C content of strain HTCC2601 was 65·4 mol%, and the predominant constituents of the cellular fatty acids were 18 : 17 (79·7 %) and 11-methyl 18 : 17 (7·5 %). The strain differed from members of the closely related genera and in its morphological, biochemical and ecological characteristics. On the basis of the taxonomic data obtained in this study, a novel genus and species, gen. nov., sp. nov., is proposed; HTCC2601 (=KCTC 12554=JCM 13377) is the type strain of .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64063-0
2006-04-01
2020-08-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/4/855.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64063-0&mimeType=html&fmt=ahah

References

  1. Cho J.-C., Giovannoni S. J. 2003a; Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α - Proteobacteria . Int J Syst Evol Microbiol 53:1031–1036 [CrossRef]
    [Google Scholar]
  2. Cho J.-C., Giovannoni S. J. 2003b; Fulvimarina pelagi gen. nov., sp. nov., a marine bacterium that forms a deep evolutionary lineage of descent in the order ‘ Rhizobiales ’. Int J Syst Evol Microbiol 53:1853–1859 [CrossRef]
    [Google Scholar]
  3. Cho J.-C., Giovannoni S. J. 2004; Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly- β -hydroxybutyrate-producing marine bacteria in the order ‘ Rhodobacterales ’. Int J Syst Evol Microbiol 54:1129–1136 [CrossRef]
    [Google Scholar]
  4. Connon S. A., Giovannoni S. J. 2002; High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl Environ Microbiol 68:3878–3885 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  7. Garrity G. M., Bell J. A., Lilburn T. 2005; Order III. Rhodobacterales ord. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2p– 161 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
    [Google Scholar]
  8. Giovannoni S., Rappé M. 2000; Evolution, diversity and molecular ecology of marine prokaryotes. In Microbial Ecology of the Oceans pp  47–84 Edited by Kirchman D. L. New York: Wiley;
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  11. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  12. Martínez-Cánovas M. J., Quesada E., Martínez-Checa F., del Moral A., Bejar V. 2004; Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the α - Proteobacteria . Int J Syst Evol Microbiol 54:1735–1740 [CrossRef]
    [Google Scholar]
  13. Martínez-Checa F., Quesada E., Martínez-Cánovas M. J., Llamas I., Bejar V. 2005; Palleronia marisminoris gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium belonging to the ‘ Alphaproteobacteria ’, isolated from a saline soil. Int J Syst Evol Microbiol 55:2525–2530 [CrossRef]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  15. Rüger H.-J., Krambeck H.-J. 1994; Evaluation of the biolog substrate metabolism system for classification of marine bacteria. Syst Appl Microbiol 17:281–288 [CrossRef]
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Microbiology pp  611–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Swofford D. 2002 paup*. Phylogenetic Analysis Using Parsimony (and other methods) Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  19. Van Trappen S., Mergaert J., Swings J. 2004; Loktanella salsilacus gen. nov., sp. nov., Loktanella fryxellensis sp. nov. and Loktanella vestfoldensis sp. nov.,new members of the Rhodobacter group, isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:1263–1269 [CrossRef]
    [Google Scholar]
  20. Wagner-Döbler I., Rheims H., Felske A., Pukall R., Tindall B. J. 2003; Jannaschia helgolandensis gen. nov., sp. nov., a novel abundant member of the marine Roseobacter clade from the North Sea. Int J Syst Evol Microbiol 53:731–738 [CrossRef]
    [Google Scholar]
  21. Wagner-Döbler I., Rheims H., Felske A., El-Ghezal A., Flade-Schroder D., Laatsch H., Lang S., Pukall R., Tindall B. J. 2004; Oceanibulbus indolifex gen. nov., sp. nov., a North Sea alphaproteobacterium that produces bioactive metabolites. Int J Syst Evol Microbiol 54:1177–1184 [CrossRef]
    [Google Scholar]
  22. Zengler K., Toledo G., Rappe M., Elkins J., Mathur E. J., Short J. M., Keller M. 2002; Cultivating the uncultured. Proc Natl Acad Sci U S A 99:15681–15686 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64063-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64063-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error