1887

Abstract

Four Gram-negative, orange-coloured, aerobic, heterotrophic bacteria were isolated from sediment samples collected on the Pacific coast of Japan near the cities of Toyohashi and Katsuura. 16S rRNA gene sequence analysis indicated that these strains form a distinct lineage within the family . The four isolates shared 99.9–100 % 16S rRNA gene sequence similarity with each other and showed 88–90.9 % similarity with their neighbours in the family . The four strains also shared high DNA–DNA reassociation values of 67–99 % with each other. All the strains grew at 37 °C but not at 4 °C, and degraded gelatin, starch and DNA. The major fatty acids were i-C, a-C i-C and i-C 3-OH. However, two common fatty acids of members of the , i-C and a-C, were absent in these strains. The DNA G+C contents of the four strains were in the range 35–37 mol%. On the basis of the polyphasic evidence, it was concluded that these strains should be classified as a novel genus and a novel species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is CKA-5 (=NBRC 100970=LMG 23247).

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2006-05-01
2019-10-14
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Bowman, J. P., McCammon, S. A., Lewis, T., Skerrat, J. H., Brown, J. L., Nichols, D. S. & McMeekin, T. A. ( 1998; ). Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense ( Dobson et al. 1993 ) as Psychroflexus gondwanense gen. nov., comb. nov. Microbiology 144, 1601–1609.[CrossRef]
    [Google Scholar]
  3. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F. ( 1978; ). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef]
    [Google Scholar]
  4. Cowan, S. T. & Steel, K. J. ( 1993; ). Manual for the Identification of Medical Bacteria, 3rd edn. London: Cambridge University Press.
  5. Dobson, S. J., Colwell, R. R., Franzmann, P. D. & McMeekin, T. A. ( 1993; ). Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43, 77–83.[CrossRef]
    [Google Scholar]
  6. Donachie, S. P., Bowman, J. P. & Alam, M. ( 2004; ). Psychroflexus tropicus sp. nov., an obligately halophilic Cytophaga–Flavobacterium–Bacteroides group bacterium from an Hawaiian hypersaline lake. Int J Syst Evol Microbiol 54, 935–940.[CrossRef]
    [Google Scholar]
  7. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  9. McCammon, S. A. & Bowman, J. P. ( 2000; ). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp., nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063.[CrossRef]
    [Google Scholar]
  10. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  11. Minamisawa, K. ( 1990; ). Division of rhizobitoxine-producing and hydrogen-uptake positive strains of Bradyrhizobium japonicum by nifDKE sequence divergence. Plant Cell Physiol 31, 81–89.
    [Google Scholar]
  12. Nakagawa, Y. & Yamasato, K. ( 1993; ). Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139, 1155–1161.[CrossRef]
    [Google Scholar]
  13. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 7 other authors ( 2003; ). Mesonia algae gen. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia soneri (Kütz) Kornm. Int J Syst Evol Microbiol 53, 1967–1971.[CrossRef]
    [Google Scholar]
  14. Nedashkovskaya, O. I., Kim, S. B., Han, S. K. & 7 other authors ( 2004; ). Algibacter lectus gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from green algae. Int J Syst Evol Microbiol 54, 1257–1261.[CrossRef]
    [Google Scholar]
  15. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Frolova, G. M., Mikhailov, V. V., Lee, K. H. & Bae, S. K. ( 2005a; ). Description of Aquimarina muelleri gen. nov., sp. nov., and proposal of the reclassification of [Cytophaga] latercula Lewin 1969 as Stanierella latercula gen. nov., comb. nov. Int J Syst Evol Microbiol 55, 225–229.[CrossRef]
    [Google Scholar]
  16. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Mikhailov, V. V., Bae, S. K. & Kim, S. ( 2005b; ). Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55, 235–238.[CrossRef]
    [Google Scholar]
  17. Perry, L. B. ( 1973; ). Gliding motility in some non-spreading flexibacteria. J Appl Bacteriol 36, 227–232.[CrossRef]
    [Google Scholar]
  18. Rüger, H.-J. & Krambeck, H.-J. ( 1994; ). Evaluation of the biolog substrate metabolism system for classification of marine bacteria. Syst Appl Microbiol 17, 281–288.[CrossRef]
    [Google Scholar]
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Sohn, J. H., Lee, J.-H., Hana, Y., Chun, J., Bae, S. K., Ahn, T.-Y. & Kim, S.-J. ( 2004; ). Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from red tide. Int J Syst Evol Microbiol 54, 675–680.[CrossRef]
    [Google Scholar]
  21. Swofford, D. L. ( 2000; ). paup* version 4. Phylogenetic Analysis Using Parsimony (*and Other Methods). Sunderland, MA: Sinauer.
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Vinuesa, P., Rademaker, J. L., de Bruijn, F. J. & Werner, D. ( 1998; ). Genotypic characterization of Bradyrhizobium strains nodulating endemic woody legumes of the Canary Islands by PCR-restriction fragment length polymorphism analysis of genes encoding 16S rRNA (16S rDNA) and 16S-23S rDNA intergenic spacers, repetitive extragenic palindromic PCR genomic fingerprinting, and partial 16S rDNA sequencing. Appl Environ Microbiol 64, 2096–2104.
    [Google Scholar]
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