1887

Abstract

Bacteria were isolated from flowers and bark of apple and pear trees at three places in Australia. In Victoria, Tasmania and Queensland, strains with white colonies on nutrient agar were screened for dome-shaped colony morphology on agar with sucrose and were found to be closely related by several criteria. The isolates were not pathogenic on apples or pears. They were characterized by a polyphasic approach including microbiological and API assays as well as fatty acid methyl ester analysis, DNA–DNA hybridization and DNA sequencing. For molecular classification, the 16S rRNA cistron and the conserved genes and of these bacteria were investigated. Together with other taxonomic criteria, the results of these studies indicate that the bacteria belong to a novel separate species, which we propose to name sp. nov., with the type strain Et1/99 (=DSM 17950=NCPPB 4357). From DNA–DNA hybridization kinetics, microbiological characteristics and nucleotide sequence analyses, this species is related to pathogenic species, but also to the epiphytic species .

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2006-12-01
2020-12-04
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