1887

Abstract

Four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. The four strains, 24K, 28K, 41KF2a and 41KF2b, were identified as members of the genus . Phylogenetic analysis based on 16S rRNA gene sequences indicated that three of the strains, 24K, 28K and 41KF2a, are very similar to one another (>98 % sequence similarity) and show a similarity of 98–99 % with and 98 % with . DNA–DNA hybridization studies showed that strains 24K, 28K and 41KF2a exhibit <70 % similarity with each other and with and . Differences in phenotypic and chemotaxonomic characteristics between the novel strains and and further confirmed that these three isolates are representatives of three separate novel species. Strain 41KF2b showed 100 % 16S rRNA gene sequence similarity to , but differed from its nearest phylogenetic neighbour in a number of phenotypic and chemotaxonomic characteristics and showed only 55 % DNA–DNA relatedness. Therefore, the four isolates represent four novel species for which the names sp. nov. (type strain, 24K=MTCC 7303=JCM 13348), sp. nov. (type strain, 28K=MTCC 7304=JCM 13347), sp. nov. (type strain, 41KF2a=MTCC 7305=JCM 13349) and sp. nov. (type strain, 41KF2b=MTCC 7306=JCM 13350) are proposed.

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2006-07-01
2024-04-23
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References

  1. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [CrossRef]
    [Google Scholar]
  2. Bruch C. W. 1967; Microbes in the upper atmosphere and beyond. In Airborne Microbes (Society for General Microbiology Symposium no. 17) pp  345–373 Edited by Gregory P. H., Monteith J. L. Cambridge: Cambridge University Press;
    [Google Scholar]
  3. Chung S., Echeverria C., Kern R., Venkateswaran K. 2000; Low-temperature H2O2 gas plasma – a sterilization process useful in space exploration technology. In Abstracts of the 100th General Meeting of the American Society for Microbiology, 2000 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  5. Gladman B. J., Burns J. A., Duncan M., Lee P., Levinson H. F. 1996; The exchange of impact ejecta between terrestrial planets. Science 271:1387–1392 [CrossRef]
    [Google Scholar]
  6. Greene V. W., Pederson P. D., Lundgren D. A., Hagberg C. A. 1964; Microbiological exploration of stratosphere: Results of six experimental flights. In Proceedings of the Atmospheric Biology Conference University of Minnesota; 13–15 April, 1964 pp  199–211 Washington, DC: National Aeronautics and Space Administration;
    [Google Scholar]
  7. Harris M. J., Wickramasinghe N. C., Lloyd D. 7 other authors 2001; The detection of living cells in stratospheric samples. In Instruments, Methods and Missions for Astrobiology IV , Proceedings of the SPIE Conference 2001 vol 4495 pp  192–198 Edited by Hoover R. B., Levin G. V., Paepe R. R., Rozanov A. Y. Bellingham, WA: SPIE;
    [Google Scholar]
  8. Holding A. J., Collee J. G. 1971; Routine biochemical tests. Methods Microbiol 6A:2–32
    [Google Scholar]
  9. Hoyle F., Wickramasinghe N. C. 1986; The case for life as a cosmic phenomenon. Nature 322:509–511 [CrossRef]
    [Google Scholar]
  10. Hoyle F., Wickramasinghe N. C. 1993 Our Place in the Cosmos London: J. M. Dent;
    [Google Scholar]
  11. Hoyle F., Wickramasinghe N. C. 1999 Astronomical Origins Of Life : Steps Towards Panspermia Dordrecht: Kluwer;
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  13. Kiran M. D., Prakash J. S. S., Annapoorni S., Dube S., Kusano T., Okuyama H., Murata N., Shivaji S. 2004; Psychrophilic Pseudomonas syringae requires trans -monounsaturated fatty acid for growth at higher temperature. Extremophiles 8:401–410 [CrossRef]
    [Google Scholar]
  14. La Duc M. T., Nicholson W., Kern R., Venkateswaran K. 2003; Microbial characterization of the Mars Odyssey spacecraft and its encapsulation facility. Environ Microbiol 5:977–985 [CrossRef]
    [Google Scholar]
  15. La Duc M. T., Satomi M., Venkateswaran K. 2004; Bacillus odysseyi sp. nov., a round spore-forming bacillus isolated from the Mars Odyssey spacecraft. Int J Syst Evol Microbiol 54:195–201 [CrossRef]
    [Google Scholar]
  16. Link L., Sawyer J., Venkateswaran K., Nicholson W. 2004; Extreme spore UV resistance of Bacillus pumilus isolates obtained from an ultraclean spacecraft assembly facility. Microb Ecol 47:159–163 [CrossRef]
    [Google Scholar]
  17. Love S. G., Brownlee D. E. 1993; A direct measurement of the terrestrial mass accretion rate of cosmic dust. Science 262:550–553 [CrossRef]
    [Google Scholar]
  18. Lysenko S. V. 1979; Microorganisms of the upper layers of the atmosphere. Mikrobiologiia 48:1066–1074 (in Russian
    [Google Scholar]
  19. Mileikowsky C., Cucinotta F. A., Wilson J. W. 7 other authors 2000; Natural transfer of viable microbes in space. Icarus 145:391–427 [CrossRef]
    [Google Scholar]
  20. Morowitz H. J., Kostelnik J. D., Yang J., Cody G. D. 2000; The origin of intermediary metabolism. Proc Natl Acad Sci U S A 97:7704–7708 [CrossRef]
    [Google Scholar]
  21. Nicholson W. L., Munakata N., Horneck G., Melosh H. J., Setlow P. 2000; Resistance of Bacilllus endospores to extreme terrestrial and extraterrestrial environments. Microbiol Mol Biol Rev 64:548–572 [CrossRef]
    [Google Scholar]
  22. Rogers L. A., Meier F. C. 1936 In Technical Paper, US Army Air Corps Stratosphere Flight of 1935 in Balloon Explorer II pp  146–151 Washington, DC: National Geographic Society;
    [Google Scholar]
  23. Sato N. S., Murata N. 1988; Membrane lipids. Methods Enzymol 167:251–259
    [Google Scholar]
  24. Shivaji S., Ray M. K., Rao N. S., Saisree L., Jagannadham M. V., Kumar G. S., Reddy G. S. N., Bhargava P. M. 1992; Sphingobacterium antarcticus sp. nov., a psychrotrophic bacterium from the soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42:102–116 [CrossRef]
    [Google Scholar]
  25. Shivaji S., Bhanu N. V., Aggarwal R. K. 2000; Identification of Yersinia pestis as the causative organism of plague in India as determined by 16S rDNA sequencing and RAPD-based genomic fingerprinting. FEMS Microbiol Lett 189:247–252 [CrossRef]
    [Google Scholar]
  26. Shivaji S., Chaturvedi P., Reddy G. S. N., Suresh K. 2005; Pedobacter himalayensis sp. nov., from the Hamta glacier located in the Himalayan mountain ranges of India. Int J Syst Evol Microbiol 55:1083–1088 [CrossRef]
    [Google Scholar]
  27. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  28. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  29. Tourova T. P., Antonov A. S. 1987; Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 19:333–355
    [Google Scholar]
  30. Venkateswaran K., Satomi M., Chung S., Kern R., Koukol R., Basic C., White D. 2001; Molecular microbial diversity of a spacecraft assembly facility. Syst Appl Microbiol 24:311–320 [CrossRef]
    [Google Scholar]
  31. Venkateswaran K., Kempf M., Chen F., Satomi M., Nicholson W., Kern R. 2003; Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are γ -radiation resistant. Int J Syst Evol Microbiol 53:165–172 [CrossRef]
    [Google Scholar]
  32. Wainwright M., Wickramasinghe N. C., Narlikar J. V., Rajaratnam P. 2003; Microorganisms cultured from stratospheric air samples obtained at 41 km. FEMS Microbiol Lett 218:161–165 [CrossRef]
    [Google Scholar]
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