1887

Abstract

Four isolates from freshly caught fish samples obtained from the South Atlantic Ocean off the South African coastline were shown to represent a novel species in the genus by means of a polyphasic taxonomic study. The four isolates had virtually identical whole-cell protein profiles, fatty acid profiles and biochemical properties. Analysis of the 16S rRNA sequence of strain LMG 23089 revealed 99.3 and 98.9 % similarity to the 16S rRNA sequences of the type strains of and , respectively. Strain LMG 23089 and the and type strains formed a stable lineage supported by a bootstrap value of 100 %. The levels of DNA–DNA hybridization towards these nearest phylogenetic neighbours were below 57 %. The absence of growth on MacConkey agar or at 37 °C (on nutrient agar), the capacity to grow in the presence of 5 % NaCl and the production of urease activity differentiate this novel taxon from and . The four isolates are formally classified as sp. nov., with strain LMG 23089 (=CCUG 51923) as the type strain. Its DNA G+C content is 33.6 mol%.

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2006-06-01
2021-02-26
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References

  1. Cowan S. T. 1974 Cowan and Steel's Manual for the Identification of Medical Bacteria , 2nd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  2. de Beer H., Hugo C. J., Jooste P. J., Willems A., Vancanneyt M., Coenye T., Vandamme P. A. R. 2005; Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant. Int J Syst Evol Microbiol 55:2149–2153 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Forsythe S. J. 2000 The Microbiology of Safe Food London: Blackwell Science;
    [Google Scholar]
  5. Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. (editors) 1981 Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Hugo C. J., Segers P., Hoste B., Vancanneyt M., Kersters K. 2003; Chryseobacterium joostei sp. nov., isolated from the dairy environment. Int J Syst Evol Microbiol 53:771–777 [CrossRef]
    [Google Scholar]
  7. Jooste P. J., Hugo C. J. 1999; The taxonomy, ecology and cultivation of bacterial genera belonging to the family Flavobacteriaceae . Int J Food Microbiol 53:81–94 [CrossRef]
    [Google Scholar]
  8. Kämpfer P., Dreyer U., Neef A., Dott W., Busse H.-J. 2003; Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53:93–97 [CrossRef]
    [Google Scholar]
  9. Kim K. K., Bae H.-S., Schumann P., Lee S.-T. 2005; Chryseobacterium daecheongense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 55:133–138 [CrossRef]
    [Google Scholar]
  10. Li Y., Kawamura Y., Fujiwara N., Naka T., Liu H., Huang X., Kobayashi K., Ezaki T. 2003; Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir. Syst Appl Microbiol 26:523–528 [CrossRef]
    [Google Scholar]
  11. MacFaddin J. F. 1980 Biochemical Tests for Identification of Medical Bacteria , 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Pavlov D., de Wet C. M. E., Grabow W. O. K., Ehlers M. M. 2004; Potentially pathogenic features of heterotrophic plate count bacteria isolated from treated and untreated drinking water. Int J Food Microbiol 92:275–287 [CrossRef]
    [Google Scholar]
  14. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  15. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  494–521 Edited by Goodfellow M., O'Donnell A. G. London: Wiley;
    [Google Scholar]
  16. Sato M. 2004; Fish inspection. In Encyclopedia of Meat Sciences vol 1 pp  474–482 Edited by Jensen W. K., Devine C., Dikeman M. Oxford: Elsevier;
    [Google Scholar]
  17. Shen F.-O., Kämpfer P., Young C.-C., Lai W.-A., Arun A. B. 2005; Chryseobacterium taichungense sp. nov., isolated from contaminated soil. Int J Syst Evol Microbiol 55:1301–1304 [CrossRef]
    [Google Scholar]
  18. Shimomura K., Kaji S., Hiraishi A. 2005; Chryseobacterium shigense sp. nov., a yellow-pigmented, aerobic bacterium isolated from a lactic acid beverage. Int J Syst Evol Microbiol 55:1903–1906 [CrossRef]
    [Google Scholar]
  19. Vandamme P., Vancanneyt M., Pot B. 10 other authors 1992; Polyphasic taxonomic study of emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42:344–356 [CrossRef]
    [Google Scholar]
  20. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B. 1994; New perspectives in the classification of flavobacteria: description of Chryseobacterium gen.nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44:827–831 [CrossRef]
    [Google Scholar]
  21. Willems A., Fernández-López M., Muñoz-Adelantado E., Goris J., De Vos P., Martínez-Romero E., Toro N., Gillis M. 2003; Description of new Ensifer strains from nodules and proposal to transfer Ensifer adhaerens Casida 1982 to Sinorhizobium as Sinorhizobium adhaerens comb. nov. Request for an Opinion. Int J Syst Evol Microbiol 53:1207–1217 [CrossRef]
    [Google Scholar]
  22. Young C.-C., Kämpfer P., Shen F.-T., Lai W.-A., Arun A. B. 2005; Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). Int J Syst Evol Microbiol 55:423–426 [CrossRef]
    [Google Scholar]
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