1887

Abstract

A reassessment was undertaken of published characteristics of the five species of anaerobic intestinal spirochaetes in the genus (, , , and ) and the two species in the genus ( and ). Comparisons were made of published descriptions, including phenotypic properties, grouping by multilocus enzyme electrophoresis, DNA base composition, DNA–DNA relative reassociation values and 16S rRNA gene sequence similarity. On the basis of extensive similarities between all the species, it is proposed to reclassify Stanton . 1997 and Stanton . 1997 in the genus , as comb. nov. (type strain ATCC 51140) and comb. nov. (type strain ATCC 51284).

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2006-05-01
2019-12-08
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References

  1. Atyeo, R. F., Stanton, T. B., Jensen, N. S., Suriyaarachichi, D. S. & Hampson, D. J. ( 1999; ). Differentiation of Serpulina species by NADH oxidase gene (nox) sequence comparisons and nox-based polymerase chain reaction tests. Vet Microbiol 67, 47–60.[CrossRef]
    [Google Scholar]
  2. Binek, M. & Szynkiewicz, Z. M. ( 1984; ). Physiological properties and classification of strains of Treponema sp. isolated from pigs in Poland. Comp Immunol Microbiol Infect Dis 7, 141–148.[CrossRef]
    [Google Scholar]
  3. Fellström, C. & Gunnarsson, A. ( 1995; ). Phenotypical characterisation of intestinal spirochaetes isolated from pigs. Res Vet Sci 59, 1–4.[CrossRef]
    [Google Scholar]
  4. Hampson, D. J. & McLaren, A. J. ( 1999; ). Experimental infection of layer hens with Serpulina intermedia causes reduced egg production and increased faecal water content. Avian Pathol 28, 113–117.[CrossRef]
    [Google Scholar]
  5. Hampson, D. J. & Stanton, T. B. ( 1997; ). Intestinal Spirochaetes in Domestic Animals and Humans. Wallingford, UK: CAB International.
  6. Harris, D. L., Glock, R. D., Christensen, C. R. & Kinyon, J. M. ( 1972; ). Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of the disease. Vet Med Small Anim Clin 67, 61–64.
    [Google Scholar]
  7. Harris, D. L., Hampson, D. J. & Glock, R. ( 1999; ). Swine dysentery. In Diseases of Swine, 8th edn, pp. 579–600. Edited by B. E. Straw, S. D'Allaire, W. L. Mengeling & D. J. Taylor. Ames, IA: Iowa State University Press.
  8. Hovind-Hougen, K., Birch-Andersen, A., Hendrik-Nielsen, R., Orholm, M., Pedersen, J. O., Teglbjærg, P. S. & Thaysen, E. H. ( 1982; ). Intestinal spirochetosis: morphological characterization and cultivation of the spirochete Brachyspira aalborgi gen. nov., sp. nov. J Clin Microbiol 16, 1127–1136.
    [Google Scholar]
  9. Kinyon, J. M. & Harris, D. L. ( 1979; ). Treponema innocens, a new species of intestinal bacteria, and emended description of the type strain of Treponema hyodysenteriae Harris et al. Int J Syst Bacteriol 29, 102–109.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Lee, J. I. & Hampson, D. J. ( 1994; ). Genetic characterisation of intestinal spirochaetes and their association with disease. J Med Microbiol 40, 365–371.[CrossRef]
    [Google Scholar]
  12. Lee, J. I., Hampson, D. J., Lymbery, A. J. & Harders, S. J. ( 1993; ). The porcine intestinal spirochaetes: identification of new genetic groups. Vet Microbiol 34, 273–285.[CrossRef]
    [Google Scholar]
  13. Leser, T. D., Møller, M., Jensen, T. K. & Jorsal, S. E. ( 1997; ). Specific detection of Serpulina hyodysenteriae and potentially pathogenic weakly β-haemolytic porcine intestinal spirochetes by polymerase chain reaction targeting 23S rDNA. Mol Cell Probes 11, 363–372.[CrossRef]
    [Google Scholar]
  14. McLaren, A. J., Trott, D. J., Swayne, D. E., Oxberry, S. L. & Hampson, D. J. ( 1997; ). Genetic and phenotypic characterization of intestinal spirochetes colonizing chickens and allocation of known pathogenic isolates to three distinct genetic groups. J Clin Microbiol 35, 412–417.
    [Google Scholar]
  15. Ochiai, S., Adachi, Y. & Mori, K. ( 1997; ). Unification of the genera Serpulina and Brachyspira, and proposals of Brachyspira hyodysenteriae comb. nov., Brachyspira innocens comb. nov. and Brachyspira pilosicoli comb. nov. Microbiol Immunol 41, 445–452.[CrossRef]
    [Google Scholar]
  16. Ochiai, S., Adachi, Y. & Mori, K. ( 1998; ). Brachyspira hyodysenteriae comb. nov., Brachyspira innocens comb. nov. and Brachyspira pilosicoli comb. nov. In Validation of Publication of New Names and New Combinations Previously Effectively Published Outside the IJSEM, List no. 64. Int J Syst Bacteriol 48, 327–328.
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Stanton, T. B. ( 1992; ). Proposal to change the genus designation Serpula to Serpulina gen. nov. containing the species Serpulina hyodysenteriae comb. nov. and Serpulina innocens comb. nov. Int J Syst Bacteriol 42, 189–190.[CrossRef]
    [Google Scholar]
  19. Stanton, T. B., Jensen, N. S., Casey, T. A., Tordoff, L. A., Dewhirst, F. E. & Paster, B. J. ( 1991; ). Reclassification of Treponema hyodysenteriae and Treponema innocens in a new genus, Serpula gen. nov., as Serpula hyodysenteriae comb. nov. and Serpula innocens comb. nov. Int J Syst Bacteriol 41, 50–58.[CrossRef]
    [Google Scholar]
  20. Stanton, T. B., Trott, D. J., Lee, J. I., McLaren, A. J., Hampson, D. J., Paster, B. J. & Jensen, N. S. ( 1996; ). Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons. FEMS Microbiol Lett 136, 181–186.[CrossRef]
    [Google Scholar]
  21. Stanton, T. B., Fournié-Amazouz, E., Postic, D., Trott, D. J., Grimont, P. A. D., Baranton, G., Hampson, D. J. & Saint Girons, I. ( 1997; ). Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov. Int J Syst Bacteriol 47, 1007–1012.[CrossRef]
    [Google Scholar]
  22. Stanton, T. B., Postic, D. & Jensen, N. S. ( 1998; ). Serpulina alvinipulli sp. nov., a new Serpulina species that is enteropathogenic for chickens. Int J Syst Bacteriol 48, 669–676.[CrossRef]
    [Google Scholar]
  23. Stephens, C. P., Oxberry, S. L., Phillips, N. D., La, T. & Hampson, D. J. ( 2005; ). The use of multilocus enzyme electrophoresis to characterise intestinal spirochaetes (Brachyspira spp.) colonising hens in commercial flocks. Vet Microbiol 107, 149–157.[CrossRef]
    [Google Scholar]
  24. Trott, D. J., Atyeo, R. F., Lee, J. I., Swayne, D. E., Stoutenburg, J. W. & Hampson, D. J. ( 1996a; ). Genetic relatedness amongst intestinal spirochaetes isolated from rats and birds. Lett Appl Microbiol 23, 431–436.[CrossRef]
    [Google Scholar]
  25. Trott, D. J., Stanton, T. B., Jensen, N. S., Duhamel, G. E., Johnson, J. L. & Hampson, D. J. ( 1996b; ). Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. Int J Syst Bacteriol 46, 206–215.[CrossRef]
    [Google Scholar]
  26. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  27. Weissenbock, H., Maderner, A., Herzog, A. M., Lussy, H. & Nowotny, N. ( 2005; ). Amplification and sequencing of Brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in Austrian pigs shows frequent solitary presence of Brachyspira murdochii. Vet Microbiol 111, 67–75.[CrossRef]
    [Google Scholar]
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