1887

Abstract

A Gram-negative, rod-shaped organism (CCUG 49520) was isolated from the midgut of the mosquito . 16S rRNA gene sequence analysis demonstrated that this isolate is unique, showing <93 % similarity to species of the families and . The quinone system consisted exclusively of ubiquinone Q-8; the polar lipid profile consisted of the major compounds phosphatidylethanolamine and phosphatidylglycerol, a moderate to minor amount of two unknown aminophospholipids, an unknown phospholipid and two unknown polar lipids; the polyamine pattern was characterized by the predominant compound 1,3-diaminopropane and showed some significant differences when compared with members of the and . On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic data, strain CCUG 49520 is considered to represent a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is CCUG 49520 (=CIP 108754).

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2006-02-01
2019-10-14
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References

  1. Altenburger, P., Kämpfer, P., Makristathis, A., Lubitz, W. & Busse, H.-J. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Busse, H.-J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  3. Busse, H.-J., Bunka, S., Hensel, A. & Lubitz, W. ( 1997; ). Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47, 698–708.[CrossRef]
    [Google Scholar]
  4. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  5. Hamana, K. ( 1996; ). Distribution of diaminopropane and acetylspermidine in Enterobacteriaceae. Can J Microbiol 42, 107–114.[CrossRef]
    [Google Scholar]
  6. Hamana, K. ( 1997; ). Polyamine distribution patterns within the families Aeromonadaceae, Vibrionaceae, Pasteurellaceae, and Halomonadaceae, and related genera of the gamma subclass of the Proteobacteria. J Gen Appl Microbiol 43, 49–59.[CrossRef]
    [Google Scholar]
  7. Kämpfer, P. ( 1990; ). Evaluation of the Titertek-Enterobac-Automated System (TTE-AS) for identification of members of the family Enterobacteriaceae. Zentralbl Bakteriol 273, 164–172.[CrossRef]
    [Google Scholar]
  8. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  10. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  12. Lindh, J. M., Terenius, O. & Faye, I. ( 2005; ). 16S rRNA gene-based identification of midgut bacteria from field-caught Anopheles gambiae sensu lato and A. funestus mosquitoes reveals new species related to known insect symbionts. Appl Environ Microbiol 71, 7217–7223.[CrossRef]
    [Google Scholar]
  13. Svoboda, P. & Harms-Ringdahl, M. ( 2002; ). Kinetics of phosphate-mediated oxidation of ferrous iron and formation of 8-oxo-2′-deoxyguanosine in solutions of free 2′-deoxyguanosine and calf thymus DNA. Biochim Biophys Acta 1571, 45–54.[CrossRef]
    [Google Scholar]
  14. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  15. Tindall, B. J. ( 1990; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  16. Wilkinson, S. G. ( 1988; ). Gram-negative bacteria. In Microbial Lipids, vol. 1, pp. 299–488. Edited by C. Ratledge & S. G. Wilkinson. New York: Academic Press.
  17. Yamamoto, S., Shinoda, S., Kawaguchi, M., Wakamatsu, K. & Makita, M. ( 1983; ). Polyamine distribution in Vibrionaceae: norspermidine as a general constituent of Vibrio species. Can J Microbiol 29, 724–728.[CrossRef]
    [Google Scholar]
  18. Yamamoto, S., Chowdhury, M. A. R., Kuroda, M., Nakano, T., Koumoto, Y. & Shinoda, S. ( 1991; ). Further study on polyamine compositions in Vibrionaceae. Can J Microbiol 37, 148–153.[CrossRef]
    [Google Scholar]
  19. Yokota, A., Akagawa-Matsushita, M., Hiraishi, A., Katayama, Y., Urakami, T. & Yamasato, K. ( 1992; ). Distribution of quinone systems in microorganisms: Gram-negative eubacteria. Bull JFCC 8, 136–171.
    [Google Scholar]
  20. Zherebilo, O., Kucheryava, N., Gvozdyak, R. I., Ziegler, D., Scheibner, M. & Auling, G. ( 2001; ). Diversity of polyamine patterns in soft rot pathogens and other plant-associated members of the Enterobacteriaceae. Syst Appl Microbiol 24, 54–62.[CrossRef]
    [Google Scholar]
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vol. , part 2, pp. 335 - 338

Two-dimensional thin-layer chromatogram of polar lipids of strain CCUG 49520 . Abbreviations: PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL1, unknown phospholipid; APL1, 2, unknown aminophospholipids; L1, 2, unknown polar lipids.



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