1887

International Journal of Systematic and Evolutionary Microbiology

Volume 56, Issue 8

sp. nov., from fish sauce in Thailand Free

Abstract

Fifteen strains of extremely halophilic bacteria were isolated from fish sauce (nam-pla) collected in Thailand at various stages of the fish-fermentation process. The isolates were strictly aerobic, spore-forming, Gram-positive rods. They grew optimally in the presence of 20–26 % NaCl. The cell-wall peptidoglycan contained -diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C and anteiso-C. Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids. The DNA G+C content was 42.1–43.1 mol%. On the basis of the 16S rRNA gene sequence, a representative strain, PS11-2, was found to be closely related to JCM 12147 (97.3 % similarity). The 15 strains were included in the same species on the basis that the levels of DNA–DNA relatedness with strain PS11-2 were greater than 70 %. They could be distinguished from and other species on the basis of several phenotypic characteristics and low levels of DNA–DNA relatedness (⩽19.4 %). Therefore, the strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PS11-2 (=JCM 12149=TISTR 1549=PCU 240).

  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63997-0
2006-08-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/8/1859.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63997-0&mimeType=html&fmt=ahah

References

  1. Albert R. A., Archambault J., Rosselló-Mora R., Tindall B. J., Matheny M. 2005; Bacillus acidicola sp. nov., a novel mesophilic, acidophilic species isolated from acidic Sphagnum peat bogs in Wisconsin. Int J Syst Evol Microbiol 55:2125–2130 [CrossRef]
     [Google Scholar]
  2. Arahal D. R., Marquez M. C., Volcani B. E., Schleifer K. H., Ventosa A. 2000; Reclassification of Bacillus marismortui as Salibacillus marismortui comb. nov. Int J Syst Evol Microbiol 50:1501–1503 [CrossRef]
     [Google Scholar]
  3. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
     [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
     [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  6. Forbes L. 1981; Rapid flagella stain. J Clin Microbiol 13:807–809
     [Google Scholar]
  7. Heydrickx M., Lebbe L., Kersters K., De Vos P., Forsyth G., Logan N. A. 1998; Virgibacillus : a new genus to accommodate Bacillus panthothenticus (Proom and Knight 1995). Emended description of Virgibacillus panthothenticus . Int J Syst Bacteriol 48:99–106 [CrossRef]
     [Google Scholar]
  8. Heyrman J., Logan N. A., Busse H.-J., Balcaen A., Lebbe L., Rodriguez-Diaz M., Swings J., De Vos P. 2003; Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of genus Salibacillus to Virgibacillus , as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus . Int J Syst Evol Microbiol 53:501–511 [CrossRef]
     [Google Scholar]
  9. Hucker G. J., Conn H. J. 1923; Method of Gram staining. Tech Bull N Y St Agric Exp Stn 93:3–37
     [Google Scholar]
  10. Jeon C. O., Lim J.-M., Lee G. S., Lee J.-M., Xu L.-H., Jiang C.-L., Kim C.-J. 2005; Lentibacillus salarius sp. nov., isolated from saline sediment in China and emended description of genus Lentibacillus . Int J Syst Evol Microbiol 55:1339–1343 [CrossRef]
     [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
     [Google Scholar]
  12. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
     [Google Scholar]
  13. Kumar S., Tamura K., Jakobson I.-B., Nei M. 2001; mega 2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
     [Google Scholar]
  14. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
     [Google Scholar]
  15. Lim J.-M., Jeon C. O., Song S. M., Kim C.-J. 2005a; Pontibacillus chungwhensis gen. nov., sp. nov. a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55:165–170 [CrossRef]
     [Google Scholar]
  16. Lim J.-M., Jeon C. O., Song S. M., Lee J.-C., Ju Y. J., Xu L. H., Jiang C.-L., Kim C.-J. 2005b; Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China. Int J Syst Evol Microbiol 55:1805–1809 [CrossRef]
     [Google Scholar]
  17. Lu J., Nogi Y., Takami H. 2001; Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205:291–297 [CrossRef]
     [Google Scholar]
  18. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
     [Google Scholar]
  19. Namwong S., Tanasupawat S., Smitinont T., Visessanguan W., Kudo T., Itoh T. 2005; Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand. Int J Syst Evol Microbiol 55:315–320 [CrossRef]
     [Google Scholar]
  20. Saito H., Miura K. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [CrossRef]
     [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  22. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . MIDI Technical Note 101: Newark, DE: MIDI;
     [Google Scholar]
  23. Schlesner H., Lawson P. A., Collins M. D., Weiss N., Wehmeyer U., Volker H., Thomm M. 2001; Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn–d-Glu-type peptidoglycan. Int J Syst Evol Microbiol 51:425–431
     [Google Scholar]
  24. Seearunruangchai A., Tanasupawat S., Keeratipibul S., Thawai C., Itoh T., Yamada Y. 2004; Identification of acetic acid bacteria isolated from fruits and related materials collected in Thailand. J Gen Appl Microbiol 50:47–53 [CrossRef]
     [Google Scholar]
  25. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. 1996; Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp.nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophilia to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
     [Google Scholar]
  26. Tamaoka J. S., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
     [Google Scholar]
  27. Tanasupawat S., Shida O., Okada S., Komagata K. 2000; Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50, 1479–1485 [CrossRef]
  28. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  29. Thornley M. J. 1960; The differentiation of Pseudomonas from other Gram-negative bacteria on the basis of arginine metabolism. J Appl Bacteriol 23:37–52 [CrossRef]
     [Google Scholar]
  30. Ventosa A., Garcia M. T., Kamekura M., Onishi H., Ruiz-Berraquero M. 1989; Bacillus halophilus sp. nov., a moderately halophilic Bacillus species. Syst Appl Microbiol 12:162–165 [CrossRef]
     [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
  32. Yoon J.-H., Kang K. H., Park Y.-H. 2002; Lentibacillus salicampi gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt field in Korea. Int J Syst Evol Microbiol 52:2043–2048 [CrossRef]
     [Google Scholar]
  33. Yoon J.-H., Oh T. K., Park Y.-H. 2004; Transfer of Bacillus halodenitrificans Denariaz et al . 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54:2163–2167 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63997-0
Loading
Lentibacillus halophilus sp. nov., from fish sauce in Thailand
Int J Syst Evol Microbiol 56, 1859 (2006); https://doi.org/10.1099/ijs.0.63997-0
/content/journal/ijsem/10.1099/ijs.0.63997-0
/content/journal/ijsem/10.1099/ijs.0.63997-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error