C6786, the clinical isolate of the ‘Oklahoma’ strain of Pseudomonas (now Burkholderia) pseudomallei, was originally isolated in 1973 from a wound infection resulting from a farming accident in Oklahoma, USA. Environmental isolates C7532 and C7533 from the Oklahoma accident site were found to match C6786. These three isolates and a clinical isolate originally identified as B. pseudomallei that was recovered from a person in Georgia, USA, involved in an automobile accident were characterized by biochemical, 16S rRNA gene sequencing, multilocus sequence typing and DNA–DNA hybridization analyses. Results indicated that these strains comprise a novel species. The name Burkholderia oklahomensis sp. nov. is proposed, with strain C6786T (=LMG 23618T=NCTC 13387T=CCUG 51349T) as the type strain.
BrennerD. J.,
McWhorterA. C.,
KnutsonJ. K. L.,
SteigerwaltA. G.1982; Escherichia vulneris : a new species of Enterobacteriaceae associated with human wounds. J Clin Microbiol 15:1133–1140
CoenyeT.,
VandammeP.2003; Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5:719–729[CrossRef]
GeeJ. E.,
SacchiC. T.,
GlassM. B.,
DeB. K.,
WeyantR. S.,
LevettP. N.,
WhitneyA. M.,
HoffmasterA. R.,
PopovicT.2003; Use of 16S rRNA gene sequencing for rapid identification and differentiation of Burkholderia pseudomallei and B. mallei
. J Clin Microbiol 41:4647–4654[CrossRef]
GodoyD.,
RandleG.,
SimpsonA. J.,
AanensenD. M.,
PittT. L.,
KinoshitaR.,
SprattB. G.2003; Multilocus sequence typing and evolutionary relationships among the causative agents of melioidosis and glanders, Burkholderia pseudomallei and Burkholderia mallei
. J Clin Microbiol 41:2068–2079[CrossRef]
MandelM.,
IgambiL.,
BergendahlJ.,
DodsonM. L.Jr,
ScheltgenE.1970; Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J Bacteriol 101:333–338
McCormickJ. B.,
WeaverR. E.,
HayesP. S.,
BoyceJ. M.,
FeldmanR. A.1977; Wound infection by an indigenous Pseudomonas pseudomallei -like organism isolated from the soil: case report and epidemiologic study. J Infect Dis 135:103–107[CrossRef]
RotzL. D.,
KhanA. S.,
LillibridgeS. R.,
OstroffS. M.,
HughesJ. M.2002; Public health assessment of potential biological terrorism agents. Emerg Infect Dis 8:225–230[CrossRef]
TomasoH.,
ScholzH. C.,
Al DahoukS.,
PittT. L.,
TreuT. M.,
NeubauerH.2004; Development of 5′ nuclease real-time PCR assays for the rapid identification of the Burkholderia mallei // Burkholderia pseudomallei complex. Diagn Mol Pathol 13:247–253[CrossRef]
WayneL. G.,
BrennerD. J.,
ColwellR. R.9 other authors1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
WuthiekanunV.,
SmithM. D.,
DanceD. A.,
WhiteN. J.1995; Isolation of Pseudomonas pseudomallei from soil in north-eastern Thailand. Trans R Soc Trop Med Hyg 89:41–43[CrossRef]
YabuuchiE.,
KosakoY.,
ArakawaM.,
HottaH.,
YanoI.1992a; Identification of Oklahoma isolate as a strain of Pseudomonas pseudomallei
. Microbiol Immunol 36:1239–1249[CrossRef]
YabuuchiE.,
KosakoY.,
OyaizuH.,
YanoI.,
HottaH.,
HashimotoY.,
EzakiT.,
ArakawaM.1992b; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36:1251–1275[CrossRef]