1887

Abstract

Two strains of Gram-negative bacteria isolated from soil by selective enrichment with nitroaromatics were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence analysis, the two strains were found to belong to the genus , within the . Strain 1B4 shared the highest sequence similarity with DSM 16610 (99.5 %) and CCM 4840 (99.3 %), and strain 2B2 with DSM 17133 (98.9 %), DSM 7231 (98.9 %) and DSM 291 (98.7 %). On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotype, including chemotaxonomic characteristics, two novel species, sp. nov. with the type strain 1B4 (=CCM 7280=DSM 16007) and sp. nov. with the type strain 2B2 (=CCM 7279=DSM 16006), are proposed. The description of was emended on the basis of additional data obtained in this study.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63988-0
2006-11-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/11/2657.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63988-0&mimeType=html&fmt=ahah

References

  1. Ajithkumar B., Ajithkumar V. P., Iriye R. 2003; Degradation of 4-amylphenol and 4-hexylphenol by a new activated sludge isolate of Pseudomonas veronii and proposal for a new subspecies status. Res Microbiol 154:17–23 [CrossRef]
    [Google Scholar]
  2. Andersen S. M., Johnsen K., Sørensen J., Nielsen P., Jacobsen C. S. 2000; Pseudomonas frederiksbergensis sp. nov., isolated from soil at a coal gasification site. Int J Syst Evol Microbiol 50:1957–1964 [CrossRef]
    [Google Scholar]
  3. Anzai Y., Kim H., Park J.-Y., Wakabayashi H., Oyaizu H. 2000; Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50:1563–1589 [CrossRef]
    [Google Scholar]
  4. Ark P. A., Tompkins C. M. 1946; Bacterial leaf blight of bird's-nest fern. Phytopathology 36:758–761
    [Google Scholar]
  5. Behrendt U., Ulrich A., Schumann P. 2003; Fluorescent pseudomonads associated with the phyllosphere of grasses; Pseudomonas trivialis sp.nov., Pseudomonas poae sp. nov. and Pseudomonas congelans sp. nov. Int J Syst Evol Microbiol 53:1461–1469 [CrossRef]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  7. Clark L. L., Dajcs J. J., McLean C. H., Bartell J. G., Stroman D. W. 2006; Pseudomonas otitidis sp. nov., isolated from patients with otic infections. Int J Syst Evol Microbiol 56:709–714 [CrossRef]
    [Google Scholar]
  8. Čechová L., Durnová E., Šikutová S., Halouzka J., Němec M. 2004; Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis. J Chromatogr B 808:248–254
    [Google Scholar]
  9. Dabboussi F., Hamze M., Elomari M., Verhille S., Baida N., Izard D., Leclerc H. 1999; Taxonomic study of bacteria isolated from Lebanese spring waters: proposal for Pseudomonas cedrella sp.nov. and P. orientalis sp. nov. Res Microbiol 150:303–316 [CrossRef]
    [Google Scholar]
  10. Dabboussi F., Hamze M., Singer E., Geoffroy V., Meyer J.-M., Izard D. 2002; Pseudomonas mosselii sp. nov., a novel species isolated from clinical specimens. Int J Syst Evol Microbiol 52:363–376
    [Google Scholar]
  11. De Soete G. 1983; A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48:621–626 [CrossRef]
    [Google Scholar]
  12. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  13. Fujii K., Urano N., Ushio H., Satomi M., Iida H., Ushio-Sata N., Kimura S. 2000; Profile of a nonylphenol-degrading microflora and its potential for bioremedial applications. J Biochem 128:909–916 [CrossRef]
    [Google Scholar]
  14. Hatayama K., Kawai S., Shoun H., Ueda Y., Nakamura A. 2005; Pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium isolated from a compost pile. Int J Syst Evol Microbiol 55:1539–1544 [CrossRef]
    [Google Scholar]
  15. Hauser E., Kämpfer P., Busse H.-J. 2004; Pseudomonas psychrotolerans sp. nov. Int J Syst Evol Microbiol 54:1633–1637 [CrossRef]
    [Google Scholar]
  16. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  17. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  18. Kotoučková L., Schumann P., Durnová E., Spröer C., Sedláček I., Neča J., Zdráhal Z., Němec M. 2004; Arthrobacter nitroguajacolicus sp. nov., a novel 4-nitroguaiacol degrading actinobacterium. Int J Syst Evol Microbiol 54:773–777 [CrossRef]
    [Google Scholar]
  19. Kwon S. W., Kim J. S., Park I. C., Yoon S. H., Park D. H., Lim C. K., Go S. J. 2003; Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53:21–27 [CrossRef]
    [Google Scholar]
  20. Maidak B. L., Cole J. R., Parker C. T. Jr 11 other authors 1999; A new version of the RDP (Ribosomal Database Project). Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  21. Miyajima K., Tanii A., Akita T. 1983; Pseudomonas fuscovaginae sp. nov., nom. rev.. Int J Syst Bacteriol 33656–657 [CrossRef]
    [Google Scholar]
  22. Mohn W. W., Wilson A. E., Bicho P., Moore E. R. B. 1999; Physiological and phylogenetic diversity of bacteria growing on resin acids. Syst Appl Microbiol 22:68–78 [CrossRef]
    [Google Scholar]
  23. Nishimori E., Kita-Tsukamoto K., Wakabayashi H. 2000; Pseudomonas plecoglossicida sp. nov., the causative agent of bacterial haemorrhagic ascites of ayu, Plecoglossus altivelis . Int J Syst Evol Microbiol 50:83–89 [CrossRef]
    [Google Scholar]
  24. Páčová Z., Kocur M. 1984; New medium for detection of esterase and gelatinase activity. Zentralbl Bakteriol Mikrobiol Hyg [A] 258:69–73
    [Google Scholar]
  25. Palleroni N. J. 1992; Introduction to the family Pseudomonadaceae . In The Prokaryotes , 2nd edn. pp  3071–3085 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  26. Pandey K. K., Mayilraj S., Chakrabarti T. 2002; Pseudomonas indica sp. nov., a novel butane-utilizing species. Int J Syst Evol Microbiol 52:1559–1567 [CrossRef]
    [Google Scholar]
  27. Park Y.-D., Lee H. B., Yi H., Kim Y., Bae K. S., Choi J. E., Jung H. S., Chun J. 2005; Pseudomonas panacis sp. nov., isolated from the surface of rusty roots of Korean ginseng. Int J Syst Evol Microbiol 55:1721–1724 [CrossRef]
    [Google Scholar]
  28. Peix A., Berge O., Rivas R., Abril A., Velázquez E. 2005; Pseudomonas argentinensis sp. nov., a novel yellow pigment-producing bacterial species, isolated from rhizospheric soil in Córdoba, Argentina. Int J Syst Evol Microbiol 55:1107–1112 [CrossRef]
    [Google Scholar]
  29. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  30. Romanenko L. A., Uchino M., Falsen E., Frolova G. M., Zhukova N. V., Mikhailov V. V. 2005a; Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 55:919–924 [CrossRef]
    [Google Scholar]
  31. Romanenko L. A., Uchino M., Falsen E., Lysenko A. M., Zhukova N. V., Mikhailov V. V. 2005b; Pseudomonas xanthomarina sp. nov., a novel bacterium isolated from marine ascidian. J Gen Appl Microbiol 51:65–71 [CrossRef]
    [Google Scholar]
  32. Savulescu T. 1947; Contribution à la classification des bacteriacées phytopathogènes. Analele Academiei Romane Series III Tom 22. Memoire 4:1–26 (in French
    [Google Scholar]
  33. Sikorski J., Stackebrandt E., Wackernagel W. 2001; Pseudomonas kilonensis sp. nov., a bacterium isolated from agricultural soil. Int J Syst Evol Microbiol 51:1549–1555
    [Google Scholar]
  34. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  35. Stanier R. Y., Palleroni N. J., Doudoroff N. 1966; The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43:159–271 [CrossRef]
    [Google Scholar]
  36. Uchino M., Shida O., Uchimura T., Komagata K. 2001; Recharacterization of Pseudomonas fulva Iizuka and Komagata 1963, and proposals of Pseudomonas parafulva sp.nov. and Pseudomonas cremoricolorata sp. nov. J Gen Appl Microbiol 46:247–261
    [Google Scholar]
  37. Verhille S., Baida N., Dabboussi F., Izard D., Leclerc H. 1999; Taxonomic study of bacteria isolated from natural mineral waters: proposal of Pseudomonas jessenii sp.nov. and Pseudomonas mandelii sp. nov. Syst Appl Microbiol 22:45–58 [CrossRef]
    [Google Scholar]
  38. Wackett L. P. 2001 The metabolic pathways of biodegradation. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , 3rd edn. release 36 22 June 2001 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; http://141.150.157.117:8080/prokPUB/index.htm
    [Google Scholar]
  39. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63988-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63988-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error