RT Journal Article SR Electronic(1) A1 Prado, Bernardo A1 Lizama, Catherine A1 Aguilera, Margarita A1 Ramos-Cormenzana, Alberto A1 Fuentes, Susana A1 Campos, Victoriano A1 Monteoliva-Sánchez, MercedesYR 2006 T1 Chromohalobacter nigrandesensis sp. nov., a moderately halophilic, Gram-negative bacterium isolated from Lake Tebenquiche on the Atacama Saltern, Chile JF International Journal of Systematic and Evolutionary Microbiology, VO 56 IS 3 SP 647 OP 651 DO https://doi.org/10.1099/ijs.0.63983-0 PB Microbiology Society, SN 1466-5034, AB A total of 52 strains of moderately halophilic bacteria isolated from hypersaline sediment of Lake Tebenquiche on the Atacama Saltern, Chile, were subjected to a taxonomic study. The morphological, physiological, biochemical and nutritional characteristics of the strains matched those described for the genus Chromohalobacter. Cells were Gram-negative, non-spore-forming, rod-shaped and motile. A black pigmentation was produced. One strain, designated LTS-4NT, grew optimally at 32 °C. Growth occurred in media containing 0·5–25 % (w/v) total salts; the optimum was 7·5 % (w/v) total salts. The pH range for growth was 5–10. The G+C content of the DNA of strain LTS-4NT was 59·8 mol%. Analysis of 16S rRNA gene sequence similarity revealed that strain LTS-4NT was closely related to Chromohalobacter species; however, DNA–DNA hybridization of representative strain LTS-4NT failed to associate the strain with any species of the genus Chromohalobacter with validly published names. Therefore, the name Chromohalobacter nigrandesensis sp. nov. is proposed. The type strain is LTS-4NT (=CECT 5315T=DSM 14323T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.63983-0