1887

Abstract

A total of 52 strains of moderately halophilic bacteria isolated from hypersaline sediment of Lake Tebenquiche on the Atacama Saltern, Chile, were subjected to a taxonomic study. The morphological, physiological, biochemical and nutritional characteristics of the strains matched those described for the genus . Cells were Gram-negative, non-spore-forming, rod-shaped and motile. A black pigmentation was produced. One strain, designated LTS-4N, grew optimally at 32 °C. Growth occurred in media containing 0·5–25 % (w/v) total salts; the optimum was 7·5 % (w/v) total salts. The pH range for growth was 5–10. The G+C content of the DNA of strain LTS-4N was 59·8 mol%. Analysis of 16S rRNA gene sequence similarity revealed that strain LTS-4N was closely related to species; however, DNA–DNA hybridization of representative strain LTS-4N failed to associate the strain with any species of the genus with validly published names. Therefore, the name sp. nov. is proposed. The type strain is LTS-4N (=CECT 5315=DSM 14323).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63983-0
2006-03-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/3/647.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63983-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R., García, M. T., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2001a; ). Transfer of Halomonas canadensis and Halomonas israelensis to the genus Chromohalobacter as Chromohalobacter canadensis comb. nov. and Chromohalobacter israelensis comb. nov. Int J Syst Evol Microbiol 51, 1443–1448.
    [Google Scholar]
  2. Arahal, D. R., García, M. T., Vargas, C., Cánovas, D., Nieto, J. J. & Ventosa, A. ( 2001b; ). Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174. Int J Syst Evol Microbiol 51, 1457–1462.
    [Google Scholar]
  3. Arahal, D. R., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2002; ). Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Int J Syst Evol Microbiol 52, 241–249.
    [Google Scholar]
  4. Elazari-Volcani, B. ( 1940; ). Studies on the microflora of the Dead Sea. PhD thesis, Hebrew University, Jerusalem, Israel.
  5. Fox, G. E., Wisotzkey, J. D. & Jurtshuk, P., Jr ( 1992; ). How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int J Syst Bacteriol 42, 166–170.[CrossRef]
    [Google Scholar]
  6. Kaye, J. Z., Márquez, M. C., Ventosa, A. & Baross, J. A. ( 2004; ). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int J Syst Evol Microbiol 54, 499–511.[CrossRef]
    [Google Scholar]
  7. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  8. Lind, E. & Ursing, J. ( 1986; ). Clinical strains of Enterobacter agglomerans (synonyms: Erwinia herbicola, Erwinia milletiae) identified by DNA–DNA hybridization. Acta Pathol Microbiol Immunol Scand Sect B Microbiol 94, 205–213.
    [Google Scholar]
  9. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  10. Oren, A. ( 2002; ). Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol 28, 56–63.[CrossRef]
    [Google Scholar]
  11. Prado, B., Del Moral, A., Quesada, E., Ríos, R., Monteoliva-Sanchez, M., Campos, V. & Ramos-Cormenzana, A. ( 1991; ). Numerical taxonomy of moderately halophilic Gram negative rods isolated from the Salar of Atacama, Chile. Syst Appl Microbiol 14, 275–281.[CrossRef]
    [Google Scholar]
  12. Quillaguamán, J., Delgado, O., Mattiasson, B. & Hatti-Kaul, R. ( 2004; ). Chromohalobacter sarecensis sp. nov., a psychrotolerant moderate halophile isolated from the saline Andean region of Bolivia. Int J Syst Evol Microbiol 54, 1921–1926.[CrossRef]
    [Google Scholar]
  13. Rodriguez-Valera, F. ( 1988; ). Characteristics and microbial ecology of hypersaline environments. In Halophilic Bacteria, vol. 1, pp. 3–30. Edited by F. Rodriguez-Valera. Boca Raton, FL: CRC Press.
  14. Sneath, P. H. A. & Sokal, R. R. ( 1973; ). Numerical Taxonomy. The Principles and Practice of Numerical Classification. San Francisco: W. H. Freeman.
  15. Sokal, R. R. & Michener, C. D. ( 1958; ). A statistical method for evaluating systematic relationships. Univ Kansas Sci Bull 38, 1409–1438.
    [Google Scholar]
  16. Subov, N. N. ( 1931; ). Oceanographical Tables. Moscow: USSR Oceanographic Institute Hydrometeorological Commission (in Russian).
  17. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  18. Ventosa, A., Quesada, E., Rodriguez-Valera, R., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  19. Ventosa, A., Gutierrez, M. C., Garcia, M. T. & Ruiz-Berraquero, F. ( 1989; ). Classification of “Chromobacterium marismortui” in a new genus, Chromohalobacter gen. nov., as Chromohalobacter marismortui comb. nov., nom. rev. Int J Syst Bacteriol 39, 382–386.[CrossRef]
    [Google Scholar]
  20. Ventosa, A., Nieto, J. J. & Oren, A. ( 1998; ). Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504–544.
    [Google Scholar]
  21. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R. ( 1998; ). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63983-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63983-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error