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Volume 56, Issue 10

Isolates of ‘ Nostocoida limicola’ Blackall 2000 should be described as three novel species of the genus , as sp. nov., sp. nov. and sp. nov. Free

Abstract

Despite differences in their morphologies, comparative analyses of 16S rRNA gene sequences revealed high levels of similarity (>94 %) between strains of the filamentous bacterium ‘ Nostocoida limicola’ and the cocci and and the rod , all isolated from activated sludge. These sequence data and their chemotaxonomic characters, including cell wall, menaquinone and lipid compositions and fingerprints of their 16S–23S rRNA intergenic regions, support the proposition that these isolates should be combined into a single genus containing six species, in the family in the . This suggestion receives additional support from DNA–DNA hybridization data and when partial sequences of the gene are compared between these strains. Even though few phenotypic characterization data were obtained for these slowly growing isolates, it is proposed, on the basis of the extensive chemotaxonomic and molecular evidence presented here, that ‘ N. limicola’ strains Ben 17, Ben 18, Ben 67, Ben 68 and Ben 74 all be placed into the species sp. nov. (type strain Ben 74=DSM 17519=NCIMB 14128), ‘ N. limicola’ strain Ben 70 into sp. nov. (type strain Ben 70=DSM 17518=NCIMB 14127) and ‘ N. limicola’ strains Ver 1 and Ver 2 into sp. nov. (type strain Ver 1=DSM 17520=NCIMB 14129).

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Keyword(s): TFO, tetrad-forming organism
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2006-10-01
2024-04-24
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Isolates of ‘Candidatus Nostocoida limicola’ Blackall et al. 2000 should be described as three novel species of the genus Tetrasphaera, as Tetrasphaera jenkinsii sp. nov., Tetrasphaera vanveenii sp. nov. and Tetrasphaera veronensis sp. nov.
Int J Syst Evol Microbiol 56, 2279 (2006); https://doi.org/10.1099/ijs.0.63978-0
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