1887

Abstract

gen. et sp. nov. is described from material collected at two sites on O'ahu, Hawaiian Islands. This alga produced golden-yellow crusts on the bark of trees and consisted of globular cells with an axial stellate chloroplast. The only form of reproduction was a specialized type of autosporulation in which a budding-like division of the mother cell produced daughter cells of different sizes. Phylogenetic analyses of the 18S rRNA gene showed that is a member of the freshwater clade of the Cladophorales/Siphonocladales lineage in the green algal class Ulvophyceae. On the basis of its unicellular habit and terrestrial habitat, this species is well differentiated from all other members of the same lineage and can be considered as the first known successful step of a member of the order Cladophorales into terrestrial habitats. The implications of the description of this species with regard to both green algal evolution and conservation of little-known tropical habitats are discussed. The holotype specimen of is GALW015489 and isotype specimens have also been deposited in the BISH and BM herbaria.

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2006-04-01
2019-10-16
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References

  1. Abe, K., Mihara, H. & Hirano, M. ( 1998; ). Characteristics of growth and carotenoid accumulation of the aerial microalga Trentepohlia aurea in liquid culture. J Mar Biotechnol 6, 53–58.
    [Google Scholar]
  2. Bakker, F. T., Olsen, J. L., Stam, W. T. & van den Hoek, C. ( 1994; ). The Cladophora complex (Chlorophyta): new views based on 18S rRNA gene sequences. Mol Phylogenet Evol 3, 365–382.[CrossRef]
    [Google Scholar]
  3. Brown, R. M. ( 1971; ). Studies of Hawaiian fresh-water and soil algae. I. The atmospheric dispersal of algae and fern spores across the island of Oahu, Hawaii. In Contributions in Phycology, pp. 175–188. Edited by B. C. Parker & R. M. Brown. Lawrence, KS: Allen Press.
  4. Brown, R. M., Larson, D. A. & Bold, H. C. ( 1964; ). Airborne algae: their abundance and heterogeneity. Science 143, 583–585.[CrossRef]
    [Google Scholar]
  5. Chapman, R. L., Buchheim, M. A., Delwiche, C. F. & 8 other authors ( 1999; ). Molecular systematics of the green algae. In Molecular Systematics of Plants II, pp. 508–540. Edited by P. S. Soltis, D. E. Soltis & J. J. Doyle. Norwell, MA: Kluwer.
  6. Donachie, S. P., Hou, S., Lee, K. S. & 11 other authors ( 2004; ). The Hawaiian archipelago: a microbial diversity hotspot. Microb Ecol 48, 509–520.[CrossRef]
    [Google Scholar]
  7. Ettl, H. & Gärtner, G. ( 1995; ). Syllabus der bode-, luf- und flechtenalgen. Stuttgart: Gustav Fischer (in German)
  8. Famá, P., Wysor, B., Kooistra, W. H. C. F. & Zuccarello, G. C. ( 2002; ). Molecular phylogeny of the genus Caulerpa (Caulerpales, Chlorophyta) inferred from chloroplast tufA gene. J Phycol 38, 1040–1050.[CrossRef]
    [Google Scholar]
  9. Fawley, M. W., Fawley, K. P. & Buchheim, M. A. ( 2004; ). Molecular diversity among communities of freshwater microchlorophytes. Microb Ecol 48, 489–499.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 29, 783–791.
    [Google Scholar]
  11. Friedl, T. ( 1995; ). Inferring taxonomic positions and testing genus level assignments in coccoid green lichen algae: a phylogenetic analysis of 18S ribosomal RNA sequences from Dictyochloropsis reticulata and from members of the genus Myrmecia (Chlorophyta, Trebouxiophyceae cl. nov.). J Phycol 31, 632–639.[CrossRef]
    [Google Scholar]
  12. Friedl, T. ( 1997; ). The evolution of the green algae. Plant Syst Evol, Suppl 11, 87–101.
    [Google Scholar]
  13. Fritsch, F. E. ( 1907; ). The subaerial and freshwater algal flora of the Tropics. A phytogeographical and ecological study. Ann Bot 21, 235–275.
    [Google Scholar]
  14. Gontcharov, A. A., Marin, B. A. & Melkonian, M. A. ( 2003; ). Molecular phylogeny of conjugating green algae (Zygnemophyceae, Streptophyta) inferred from SSU rDNA sequence comparisons. J Mol Evol 56, 89–104.[CrossRef]
    [Google Scholar]
  15. Hamby, R. K., Sims, L., Issel, L. & Zimmer, E. ( 1988; ). Direct ribosomal RNA sequencing: optimization of extraction and sequencing methods for work with higher plants. Plant Mol Biol Rep 6, 175–192.[CrossRef]
    [Google Scholar]
  16. Handa, S., Nakahara, M., Tsubota, H., Deguchi, H. & Nakano, T. ( 2003; ). A new aerial alga, Stichococcus ampulliformis sp. nov., (Trebouxiophyceae, Chlorophyta) from Japan. Phycol Res 51, 203–210.[CrossRef]
    [Google Scholar]
  17. Hanyuda, T., Wakana, I., Arai, S., Miyaji, K., Watano, Y. & Ueda, K. (2002; ). Phylogenetic relationships within Cladophorales (Ulvophyceae, Chlorophyta) inferred from 18S rRNA gene sequences, with special reference to Aegagropila linnaei. J Phycol 38, 564–571.[CrossRef]
    [Google Scholar]
  18. Henley, W. J., Hironaka, J. L., Guillou, L., Buchheim, M. A., Buchheim, J. A., Fawley, M. W. & Fawley, K. P. ( 2004; ). Phylogenetic analysis of the “Nannochloris-like” algae and diagnoses of Picochlorum oklahomensis gen. et sp. nov. (Trebouxiophyceae, Chlorophyta). Phycologia 43, 641–652.[CrossRef]
    [Google Scholar]
  19. Hindák, F. ( 1976; ). Marvania geminata gen. nov. et sp. nov., a new green alga. Algol Stud 16, 261–270.
    [Google Scholar]
  20. Ho, K. K., Tan, K. H. & Wee, Y. C. ( 1983; ). Growth conditions of Trentepohlia odorata (Chlorophyta, Ulotrichales). Phycologia 22, 303–308.[CrossRef]
    [Google Scholar]
  21. Hoffmann, L. ( 1989; ). Algae of terrestrial habitats. Bot Rev 55, 77–105.[CrossRef]
    [Google Scholar]
  22. Holmgren, P. K., Holmgren, N. H. & Barnett, L. C. ( 1990; ). Index Herbariorum. Part I: The Herbaria of the World. Koenigstein, Germany: Koeltz Scientific Books.
  23. Huelsenbeck, J. P. & Ronquist, F. ( 2001; ). mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17, 754–755.[CrossRef]
    [Google Scholar]
  24. Kooistra, W. H. C. F., Olsen, J. L., Stam, W. T. & van den Hoek, C. ( 1993; ). Problems relating to species sampling in phylogenetic studies: an example of non-monophyly in Cladophoropsis and Struvea (Siphonocladales, Chlorophyta). Phycologia 32, 419–428.[CrossRef]
    [Google Scholar]
  25. Krienitz, L., Hegewald, E., Hepperle, D. & Wolf, A. ( 2003; ). The systematics of coccoid green algae: 18S rRNA gene sequence data versus morphology. Biologia Bratislava 58, 437–446.
    [Google Scholar]
  26. Krienitz, L., Hegewald, E. H., Hepperle, D., Huss, V. A. R., Rohr, T. & Wolf, M. ( 2004; ). Phylogenetic relationship of Chlorella and Parachlorella gen. nov. (Chlorophyta, Trebouxiophyceae). Phycologia 43, 529–542.[CrossRef]
    [Google Scholar]
  27. Kristiansen, J. ( 1996; ). Dispersal of freshwater algae – a review. Hydrobiologia 336, 151–157.[CrossRef]
    [Google Scholar]
  28. Leliaert, F., Rousseau, F., De Reviers, B. & Coppejans, E. ( 2003; ). Phylogeny of the Cladophorophyceae (Chlorophyta) inferred from partial LSU rRNA gene sequences: is the recognition of a separate order Siphonocladales justified? Eur J Phycol 38, 233–246.[CrossRef]
    [Google Scholar]
  29. Lewis, L. A. & McCourt, R. M. ( 2004; ). Green algae and the origin of land plants. Am J Bot 91, 1535–1556.[CrossRef]
    [Google Scholar]
  30. López-Bautista, J. M. & Chapman, R. L. ( 2003; ). Phylogenetic affinities of the Trentepohliales inferred from small-subunit rDNA. Int J Syst Evol Microbiol 53, 2099–2106.[CrossRef]
    [Google Scholar]
  31. López-Bautista, J. M., Waters, D. A. & Chapman, R. L. ( 2002; ). The Trentepohliales revisited. Constancea 83.1 (http://ucjeps.berkeley.edu/constancea/83/lopez_etal/trentepohliales.html)
  32. Maddison, D. R. & Maddison, W. P. ( 2001; ). MacClade 4: Analysis of phylogeny and character evolution, version 4.03. Sunderland, MA: Sinauer Associates.
  33. Matsuyama, K., Matsuoka, T., Miyaji, K., Tanaka, J. & Aruga, Y. ( 1998; ). Ultrastructure of the pyrenoid in the family Cladophoraceae (Cladophorales, Chlorophyta). J Jpn Bot 73, 279–286.
    [Google Scholar]
  34. McCourt, R. M., Karol, K. G., Bell, J., Helm-Bychowski, K. M., Grajewska, A., Wojciechowski, M. F. & Hoshaw, R. W. ( 2000; ). Phylogeny of the conjugating green algae (Zygnemophyceae) based on rbc L sequences. J Phycol 36, 747–758.[CrossRef]
    [Google Scholar]
  35. Mueller-Dombois, D. ( 2002; ). Forest vegetation across the tropical Pacific: a biogeographically complex region with many analogous environments. Plant Ecol 163, 155–176.[CrossRef]
    [Google Scholar]
  36. Neustupa, J. & Šejnohová, L. ( 2003; ). Marvania aerophytica sp. nov., a new subaerial tropical green alga. Biologia 58, 503–507.
    [Google Scholar]
  37. Nienow, J. A. ( 1996; ). Ecology of subaerial algae. Nova Hedwigia Beih 112, 537–552.
    [Google Scholar]
  38. O'Kelly, C. J., Wysor, B. & Bellows, W. K. ( 2004; ). Gene sequence diversity and the phylogenetic position of algae assigned to the genera Phaeophila and Ochlochaete (Ulvophyceae, Chlorophyta). J Phycol 40, 789–799.[CrossRef]
    [Google Scholar]
  39. Posada, D. & Crandall, K. A. ( 1998; ). modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.[CrossRef]
    [Google Scholar]
  40. Reymond, O. L., Hindák, F. & Sluiman, H. J. ( 1986; ). Morphologie cellulaire et cycle de développement chez l'algue verte Marvania geminata Hindák (1976) . Arch Sci Genève 39, 243–255 (in French).
    [Google Scholar]
  41. Rindi, F., McIvor, L. & Guiry, M. D. ( 2004; ). The Prasiolales (Chlorophyta) of Atlantic Europe: an assessment based on morphological, molecular, and ecological data, including the characterization of Rosenvingiella radicans (Kützing) comb. nov. J Phycol 40, 977–997.[CrossRef]
    [Google Scholar]
  42. Rindi, F., Sherwood, A. R. & Guiry, M. D. ( 2005; ). Taxonomy and distribution of Trentepohlia and Printzina (Trentepohliales, Chlorophyta) in the Hawaiian Islands. Phycologia 44, 270–284.[CrossRef]
    [Google Scholar]
  43. Rosas, I., Roy-Ocotla, G., Mosiño, P., Baez, A. & Rivera, L. ( 1987; ). Abundance and heterogeneity of algae in the Mexico City atmosphere. Geofis Int 26, 359–373.
    [Google Scholar]
  44. Roy-Ocotla, G. & Carrera, J. ( 1993; ). Aeroalgae: responses to some aerobiological questions. Grana 32, 48–56.[CrossRef]
    [Google Scholar]
  45. Sakai, Y. ( 1964; ). The species of Cladophora from Japan and its vicinity. Sci Pap Inst Algol Res Fac Sci Hokkaido Univ 5, 1–104.
    [Google Scholar]
  46. Sherwood, A. R. ( 2004; ). Bibliographic checklist of the non-marine algae of the Hawaiian Islands. Bishop Mus Occ Pap Series 80, 1–23.
    [Google Scholar]
  47. Sherwood, A. R., Garbary, D. J. & Sheath, R. G. ( 2000; ). Assessing the phylogenetic position of the Prasiolales (Chlorophyta) using rbcL and 18S rRNA gene sequence data. Phycologia 39, 139–146.[CrossRef]
    [Google Scholar]
  48. Siefermann-Harms, D. ( 1987; ). The light-harvesting and protective functions of carotenoids in photosynthetic membranes. Physiol Plant 69, 561–568.[CrossRef]
    [Google Scholar]
  49. Sluiman, H. J. & Reymond, O. L. ( 1987; ). Cell division in the green microalga Marvania geminata: semi-exogenous autosporogenesis, role of coated pit-microtubule complexes, and systematic significance. Acta Bot Neerl 36, 231–245.[CrossRef]
    [Google Scholar]
  50. Swofford, D. L. ( 2000; ). paup*: Phylogenetic analysis using parsimony (* and other methods), version 4. Sunderland, MA: Sinauer Associates.
  51. Tan, I. H., Blomster, J., Hansen, G., Leskinen, E., Maggs, C. A., Mann, D. G., Sluimam, H. J. & Stanhope, M. J. ( 1999; ). Molecular phylogenetic evidence for a reversible morphogenetic switch controlling the gross morphology of two common genera of green seaweeds, Ulva and Enteromorpha. Mol Biol Evol 16, 1011–1018.[CrossRef]
    [Google Scholar]
  52. Van den Hoek, C., Mann, D. G. & Jahns, H. M. ( 1995; ). Algae – an Introduction to Phycology. Cambridge: Cambridge University Press.
  53. Yang, W. X. ( 1989; ). Observations on pyrenoid ultrastructure of Cladophora conchopheria (Charophyceae). Jpn J Phycol 37, 57–59.
    [Google Scholar]
  54. Yoshii, Y., Hanyuda, T., Wakana, K., Miyaji, K., Arai, S., Ueda, K. & Inouye, I. ( 2004; ). Carotenoid compositions of Cladophora balls (Aegagropila linnaei) and some members of the Cladophorales (Ulvophyceae, Chlorophyta): their taxonomic and evolutionary implication. J Phycol 40, 1170–1177.[CrossRef]
    [Google Scholar]
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