A Gram-negative, facultatively anaerobic bacterial strain designated GPTSA-6T was isolated from a water sample collected from a warm spring in Assam, India. Preliminary analysis of the 16S rRNA gene sequence of this isolate revealed its affiliation to the family Aeromonadaceae. Detailed characterization using a polyphasic approach indicated that strain GPTSA-6T is most closely related to Aeromonas sobria but differs significantly from existing members of the genus Aeromonas. Analysis of the almost-complete (1430 nt) 16S rRNA gene sequence of this strain revealed that its closest relative (99.23 % similarity) is an uncultured bacterial clone, A-8, isolated from an algal bloom. Of the taxa with validly published names, Aeromonas sobria ATCC 43979T showed the highest level of sequence similarity (95.13 %) with respect to strain GPTSA-6T, followed by Aeromonas molluscorum 848TT and Aeromonas popoffii LMG 17541T (95.04 % similarity in both cases). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, it can be concluded that strain GPTSA-6T represents a novel species of the genus Aeromonas, for which the name Aeromonas sharmana sp. nov. is proposed. The type strain is GPTSA-6T (=MTCC 7090T=DSM 17445T).
BaumannP.,
BowditchR. D.,
BaumannL.,
BeamanB.1983; Taxonomy of marine Pseudomonas species: P. stanieri sp. nov.; P. perfectomarina sp. nov., nom. rev.; P. nautica ; and P. doudoroffii
. Int J Syst Bacteriol 33:857–865[CrossRef]
BrownG. R.,
SutcliffeI. C.,
CummingsS. P.2001; Reclassification of [ Pseudomonas ] doudoroffii (Baumann et al . 1983) into the genus Oceanimonas gen. nov. as Oceanimonas doudoroffii comb. nov., and description of a phenol-degrading bacterium from estuarine water as Oceanimonas baumannii sp. nov. Int J Syst Evol Microbiol 51:67–72
FelsensteinJ.1993phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
Fischer-RomeroC.,
TindallB. J.,
JüttinerF.1996; Tolumonas auensis gen. nov., sp. nov., a toluene-producing bacterium from anoxic sediments of a freshwater lake. Int J Syst Bacteriol 46:183–188[CrossRef]
Harf-MonteilC.,
Le FlècheA.,
RiegelP.,
PrévostG.,
BermondD.,
GrimontP. A. D.,
MonteilH.2004; Aeromonas simiae sp. nov., isolated from monkey faeces. Int J Syst Evol Microbiol 54:481–485[CrossRef]
HoltJ. G.,
KriegN. R.,
SneathP. H. A.,
StaleyJ. T.,
WilliamsS. T.1994Bergey's Manual of Determinative Bacteriology , 9th edn. Baltimore: Williams & Wilkins;
HuysG.,
VancanneytM.,
CoopmanR.,
JanssenP.,
FalsenE.,
AltweggM.,
KerstersK.1994; Cellular fatty acid composition as chemotaxonomic marker for the differentiation of phenospecies and hybridization groups in the genus Aeromonas
. Int J Syst Bacteriol 44:651–658[CrossRef]
HuysG.,
KämpferP.,
AltweggM.7 other authors1997; Aeromonas popoffii sp. nov., a mesophilic bacterium isolated from drinking water production plants and reservoirs. Int J Syst Bacteriol 47:1165–1171[CrossRef]
KasugaI.,
NakajimaF.,
FurumaiH.2003; Analysis of dissolved organic matter and bacterial community in degradation of algal bloom by EEMS and PCR-DGGE. J Jpn Soc Water Environ 26:171–174 (in Japanese [CrossRef]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120[CrossRef]
MurrayR. G. E.,
DoetschR. N.,
RobinowC. F.1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp 21–41 Edited by
GerhardP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
PopoffM.1984; Genus III Aeromonas Kluvyer and Van Niel 1936, 398AL
. In Bergey's Manual of Systematic Bacteriologyvol. 1 pp 545–548 Edited by
KriegN. R.,
HoltJ. G.
Baltimore: Williams & Wilkins;
PowersE. M.1995; Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeast. Appl Environ Microbiol 61:3756–3758
SahaP.,
KrishnamurthiS.,
MayilrajS.,
PrasadG. S.,
BoraT. C.,
ChakrabartiT.2005; Aquimonas voraii gen. nov., sp. nov., a novel gammaproteobacterium isolated from a warm spring of Assam, India. Int J Syst Evol Microbiol 55:1491–1495[CrossRef]
SmibertR. M.,
KriegN. R.1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654 Edited by
GerhardtP.,
MurrayR. G. E.,
WoodW. A.,
KriegN. R.
Washington, DC: American Society for Microbiology;
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
Van de PeerY.,
De WachterR.1997; Construction of evolutionary distance trees with treecon for Windows: accounting for variation in nucleotide substitution rate among sites. Comput Appl Biosci 13:227–230