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Abstract

An isolate of purple non-sulfur bacteria was obtained from an acidic peat bog and designated strain RS. The colour of cell suspensions of this bacterium growing in the light under anaerobic conditions is purplish red. Cells of strain RS are rod-shaped, 0.8–1.0 μm wide and 2.0–6.0 μm long, motile by means of polar flagella, reproduce by budding and have a tendency to form rosette-like clusters in older cultures. The cells contain lamellar intracytoplasmic membranes underlying, and parallel to, the cytoplasmic membrane. The photosynthetic pigments are bacteriochlorophyll and carotenoids; the absorption spectrum of living cells shows maxima at 377, 463, 492, 527, 592, 806 and 867 nm. The cells grow photoheterotrophically under anaerobic or microaerobic conditions with various organic carbon sources or grow photolithoautotrophically with H and CO. Strain RS is a moderately acidophilic organism exhibiting growth at pH values between 4.8 and 7.0 (with an optimum at pH 5.2–5.5). The major fatty acids are 16 : 17 and 18 : 17; the major quinones are Q-10 and Q-9. The DNA G+C content of strain RS is 62.6 mol%. Analysis of the 16S rRNA gene sequence revealed that the novel isolate is most closely related (97.3 % sequence similarity) to the type strain ATCC 25092 of the moderately acidophilic purple non-sulfur bacterium , formerly named . However, in contrast to , strain RS is not capable of aerobic growth in the dark, has no spirilloxanthin among the carotenoids and differs in the pattern of substrate utilization. The value for DNA–DNA hybridization between strain RS and ATCC 25092 is only 22 %. Thus, strain RS represents a novel species of the genus , for which the name sp. nov. is proposed. Strain RS (=DSM 16996=VKM B-2361) is the type strain.

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2006-06-01
2019-12-15
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vol. , part 6, pp. 1397 - 1402

Absorption spectrum of a cell homogenate of phototrophically grown strain RS .

Influence of medium pH on the growth of strain RS .

[PDF file of Supplementary Figs S1 and S2](33 KB)



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