1887

Abstract

A novel anaerobic, thermophilic, CO-utilizing bacterium, strain Kar, was isolated from a hot spring of Karymskoe Lake, Kamchatka Peninsula. The cells of the novel isolate were Gram-positive, spore-forming, short rods. The bacterium grew chemolithoautotrophically on CO, producing equimolar quantities of H and CO (according to the equation CO + HO → CO + H), and in the absence of CO, under N in the gas phase, chemoorganoheterotrophically with yeast extract, sucrose or pyruvate. Growth was observed in the temperature range 50–70 °C, with an optimum at 60 °C, and in the pH range 6·2–8·0, with an optimum at pH 6·8. The micro-organism did not grow on solid media; it was able to grow only in semi-solid medium containing 0·5 % agar. The generation time under optimal conditions for chemolithoautotrophic growth was 1 h. The G+C content of the DNA was 46·5±1 mol%. Growth was completely inhibited by penicillin, novobiocin, streptomycin, kanamycin and neomycin. Analysis of the 16S rRNA gene sequence showed that the isolate should be assigned to the genus . On the basis of the results of DNA–DNA hybridization and morphological and physiological analyses, strain Kar represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Kar (=DSM 16521=VKM B-2358).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63961-0
2006-04-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/4/797.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63961-0&mimeType=html&fmt=ahah

References

  1. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  2. Fardeau M. L., Salinas M. B., L'Haridon S., Jeanthon C., Verhe F., Cayol J. L., Patel B. K., Garcia J. L., Ollivier B. 2004; Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus : reassignment of T. subterraneus , Thermoanaerobacter yonseiensis , Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies. Int J Syst Evol Microbiol 54:467–474 [CrossRef]
    [Google Scholar]
  3. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  4. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  5. Pfennig N., Lippert K. D. 1966; über das Vitamin B12-Bedürfnis phototropher Schwefelbakterien. Arch Mikrobiol 55:245–256 (in German [CrossRef]
    [Google Scholar]
  6. Sokolova T. G., González J. M., Kostrikina N. A., Chernyh N. A., Tourova T. P., Kato C., Bonch-Osmolovskaya E. A., Robb F. T. 2001; Carboxydobrachium pacificum gen. nov., sp. nov., a new anaerobic, thermophilic, CO-utilizing marine bacterium from Okinawa Trough. Int J Syst Evol Microbiol 51:141–149
    [Google Scholar]
  7. Sokolova T. G., Kostrikina N. A., Chernyh N. A., Tourova T. P., Kolganova T. V., Bonch-Osmolovskaya E. A. 2002; Carboxydocella thermautotrophica gen. nov., sp. nov. a novel anaerobic, CO-utilizing thermophile from a Kamchatkan hot spring. Int J Syst Evol Microbiol 52:1961–1967 [CrossRef]
    [Google Scholar]
  8. Sokolova T. G., Gonzáles J. M., Kostrikina N. A., Chernyh N. A., Slepova T. V., Bonch-Osmolovskaya E. A., Robb F. T. 2004a; Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park. Int J Syst Evol Microbiol 54:2353–2359 [CrossRef]
    [Google Scholar]
  9. Sokolova T. G., Jeanthon C., Kostrikina N. A., Chernyh N. A., Lebedinsky A. V., Stackebrandt E., Bonch-Osmolovskaya E. A. 2004b; The first evidence of anaerobic CO oxidation coupled with H2 production by a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent. Extremophiles 8:317–323
    [Google Scholar]
  10. Sokolova T. G., Kostrikina N. A., Chernyh N. A., Kolganova T. V., Tourova T. P., Bonch-Osmolovskaya E. A. 2005; Thermincola carboxydiphila gen. nov., sp. nov. a novel anaerobic, carboxydotrophic, hydrogenogenic bacterium from a hot spring of the Lake Baikal area. Int J Syst Evol Microbiol 55:2069–2073 [CrossRef]
    [Google Scholar]
  11. Svetlichny V. A., Sokolova T. G., Gerhardt M., Ringpfeil M., Kostrikina N. A., Zavarzin G. A. 1991; Carboxydothermus hydrogenoformans gen. nov., sp. nov. a CO-utilizing thermophilic anaerobic bacterium from hydrothermal environments of Kunashir Island. Syst Appl Microbiol 14:254–260 [CrossRef]
    [Google Scholar]
  12. Trüper H. G., Schlegel H. G. 1964; Sulfur metabolism in Thiorhodaceae . I. Quantitative measurements on growing cells of Chromatium okenii . Antonie van Leeuwenhoek 30:225–238 [CrossRef]
    [Google Scholar]
  13. Wolin E. A., Wolin M. J., Wolfe R. S. 1963; Formation of methane by bacterial extracts. J Biol Chem 238:2882–2886
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.63961-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63961-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error