1887

Abstract

A novel anaerobic, thermophilic, CO-utilizing bacterium, strain Kar, was isolated from a hot spring of Karymskoe Lake, Kamchatka Peninsula. The cells of the novel isolate were Gram-positive, spore-forming, short rods. The bacterium grew chemolithoautotrophically on CO, producing equimolar quantities of H and CO (according to the equation CO + HO → CO + H), and in the absence of CO, under N in the gas phase, chemoorganoheterotrophically with yeast extract, sucrose or pyruvate. Growth was observed in the temperature range 50–70 °C, with an optimum at 60 °C, and in the pH range 6·2–8·0, with an optimum at pH 6·8. The micro-organism did not grow on solid media; it was able to grow only in semi-solid medium containing 0·5 % agar. The generation time under optimal conditions for chemolithoautotrophic growth was 1 h. The G+C content of the DNA was 46·5±1 mol%. Growth was completely inhibited by penicillin, novobiocin, streptomycin, kanamycin and neomycin. Analysis of the 16S rRNA gene sequence showed that the isolate should be assigned to the genus . On the basis of the results of DNA–DNA hybridization and morphological and physiological analyses, strain Kar represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Kar (=DSM 16521=VKM B-2358).

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2006-04-01
2020-10-01
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