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Abstract

Within a collection of marine strains that were shown to contain the photosynthesis reaction-centre genes and , a novel group of alphaproteobacteria was found and was characterized phenotypically. The 16S rRNA gene sequence data suggested that the strains belonged to the order and were closest (98·5 % sequence similarity) to the recently described species . The cells contained bacteriochlorophyll and a carotenoid, presumably spheroidenone, in small to medium amounts. Cells of the novel strains were small rods and were motile by means of single polarly inserted flagella. Good growth occurred in complex media with 0·5–7·0 % sea salts, at 25–33 °C (optimum, 31 °C) and at pH values in the range 6–9. With the exception of acetate and malate, organic carbon sources tested supported poor growth or no growth at all. Growth factors were required; these were provided by small amounts of yeast extract, but not by standard vitamin solutions. Growth occurred under aerobic to microaerobic conditions, but not under anaerobic conditions, either in the dark or light. Nitrate was not reduced. Photosynthetic pigments were formed at low to medium salt concentrations, but not at the salt concentration of sea water (3·5 %). On the basis of smaller cell size, different substrate utilization profile and photosynthetic pigment content, the novel strains can be classified as representatives of a second species of , for which the name sp. nov. is proposed. The type strain of sp. nov. is DFL-43 (=DSM 17068=NCIMB 14078).

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2006-04-01
2019-12-13
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References

  1. Ahrens, R. ( 1968; ). Taxonomische Untersuchungen an sternbildenden Agrobacterium-Arten aus der westlichen Ostsee. Kieler Meeresforsch 24, 147–173 (in German).
    [Google Scholar]
  2. Allgaier, M., Uphoff, H., Felske, A. & Wagner-Döbler, I. ( 2003; ). Aerobic anoxygenic photosynthesis in Roseobacter clade bacteria from diverse marine habitats. Appl Environ Microbiol 69, 5051–5059.[CrossRef]
    [Google Scholar]
  3. Biebl, H., Tindall, B. J., Koblizek, M., Lünsdorf, H., Pukall, R. & Wagner-Döbler, I. ( 2005; ). Dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. Int J Syst Evol Microbiol 55, 1089–1096.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, WA, USA.
  5. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  6. Kolber, Z. S., Plumley, F. G., Lang, A. S. & 7 other authors ( 2001; ). Contribution of aerobic photoheterotrophic bacteria to the carbon cycle in the ocean. Science 292, 2492–2495.[CrossRef]
    [Google Scholar]
  7. Labrenz, M., Collins, M. D., Lawson, P. A., Tindall, B. J., Schumann, P. & Hirsch, P. ( 1998; ). Antarctobacter heliothermus gen. nov., sp. nov., a budding bacterium from hypersaline and heliothermal Ekho Lake. Int J Syst Bacteriol 48, 1363–1372.[CrossRef]
    [Google Scholar]
  8. Maidak, B. L., Cole, J. R., Lilburn, T. G. & 7 other authors ( 2001; ). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef]
    [Google Scholar]
  9. Peix, A., Rivas, R., Trujillo, M. E., Vancanneyt, M., Velazquez, E. & Willems, A. ( 2005; ). Reclassification of Agrobacterium ferrugineum LMG 128 as Hoeflea marina gen. nov., sp. nov. Int J Syst Evol Microbiol 55, 1163–1166.[CrossRef]
    [Google Scholar]
  10. Rüger, H.-J. & Höfle, M. ( 1992; ). Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev. Int J Syst Bacteriol 42, 133–143.[CrossRef]
    [Google Scholar]
  11. Schwalbach, M. S. & Fuhrman, J. A. ( 2005; ). Wide-ranging abundance of aerobic anoxygenic phototrophic bacteria in the world ocean revealed by epifluorescence microscopy and quantitative PCR. Limnol Oceanogr 50, 620–628.[CrossRef]
    [Google Scholar]
  12. Tindall, B. J. ( 1990; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  13. Uchino, Y., Hirahata, A., Yakota, A. & Sugiyama, J. ( 1998; ). Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kielensis gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44, 201–210.[CrossRef]
    [Google Scholar]
  14. Yurkov, V., Jappé, J. & Verméglio, A. ( 1996; ). Tellurite resistance by obligately aerobic photosynthetic bacteria. Appl Environ Microbiol 62, 4195–4198.
    [Google Scholar]
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