1887

Abstract

Strain EJ-46, a novel pleomorphic, aerobic, extremely halophilic member of the was isolated from sediment of the saline Lake Ejinor, in Inner Mongolia, China. This organism was neutrophilic and required at least 15 % (2·5 M) NaCl for growth. MgCl was not required. The isolate was able to grow at pH 6·0–9·0. Optimum growth occurred in media containing 20 % (3·4 M) NaCl at pH 7·0–7·5. Polar lipid analysis revealed the presence of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, derived from both CC and CC glycerol diethers. Four glycolipids were detected, one of which may be novel. The DNA G+C content was 60·3 mol%. 16S rRNA gene analysis revealed that strain EJ-46 was a member of the phylogenetic group defined by the family , and the highest 16S rRNA gene similarity values of 94·9 and 94·8 % were obtained with the haloalkaliphilic species of the genus , and , respectively. Based on the phenotypic, genotypic and phylogenetic analyses, it is proposed that the novel isolate should be classified as representing a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is EJ-46 (=CGMCC 1.4248=CECT 7098).

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2006-04-01
2024-10-11
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References

  1. Arahal D. R., Dewhirst F. E., Paster B. J., Volcani B. E., Ventosa A. 1996; Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Appl Environ Microbiol 62:3779–3786
    [Google Scholar]
  2. Denner E. B. M., McGenity T. J., Busse H.-J., Grant W. D., Wanner G., Stan-Lotter H. 1994; Halococcus salifodinae sp. nov., an archaeal isolate from an Austrian salt mine. Int J Syst Bacteriol 44:774–780 [CrossRef]
    [Google Scholar]
  3. Fan H., Xue Y., Ma Y., Ventosa A., Grant W. D. 2004; Halorubrum tibetense sp. nov., a novel haloalkaliphilic archaeon from Lake Zabuye in Tibet, China. Int J Syst Evol Microbiol 54:1213–1216 [CrossRef]
    [Google Scholar]
  4. Feng J., Zhou P.-J., Liu S.-J. 2004; Halorubrum xinjiangense sp. nov., a novel halophile isolated from saline lakes in China. Int J Syst Evol Microbiol 54:1789–1791 [CrossRef]
    [Google Scholar]
  5. Feng J., Zhou P., Zhou Y.-G., Liu S.-J., Warren-Rhodes K. 2005; Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xinjiang, China. Int J Syst Evol Microbiol 55:149–152 [CrossRef]
    [Google Scholar]
  6. Franzmann P. D., Stackebrandt E., Sanderson K., Volkman J. K., Cameron D. E., Stevenson P. L., McMeekin T. A., Burton H. R. 1988; Halobacterium lacusprofundi sp. nov., a halophilic bacterium isolated from Deep Lake, Antarctica. Syst Appl Microbiol 11:20–27 [CrossRef]
    [Google Scholar]
  7. Gonzalez C., Gutierrez C., Ramirez C. 1978; Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24:710–715 [CrossRef]
    [Google Scholar]
  8. Grant W. D. 2001a; Genus I. Halobacterium Elazari-Volcani 1957, 207,AL emend. Larsen and Grant 1989, 2222. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  301–305 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  9. Grant W. D. 2001b; Genus IV. Halococcus Schoop 1935a, 817AL. In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol 1 pp  311–314 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
  10. Grant W. D., Kamekura M., McGenity T. J., Ventosa A. 2001; Order I. Halobacteriales Grant and Larsen 1989b, 495VP (Effective publication: Grant and Larsen 1989a, 2216. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  294–334 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  11. Hezayen F. F., Rehm B. H. A., Tindall B. J., Steinbüchel A. 2001; Transfer of Natrialba asiatica B1T to Natrialba taiwanensis sp. nov. and description of Natrialba aegyptiaca sp. nov., a novel extremely halophilic, aerobic, non-pigmented member of the Archaea from Egypt that produces extracellular poly(glutamic acid). Int J Syst Evol Microbiol 51:1133–1142 [CrossRef]
    [Google Scholar]
  12. Hezayen F. F., Tindall B. J., Steinbüchel A., Rehm B. H. A. 2002; Characterization of a novel halophilic archaeon, Halobiforma haloterrestris gen. nov., sp. nov. and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. Int J Syst Evol Microbiol 52:2271–2280 [CrossRef]
    [Google Scholar]
  13. Ihara K., Watanabe S., Tamura T. 1997; Haloarcula argentinensis sp. nov. and Haloarcula mukohataei sp. nov., two new extremely halophilic archaea collected in Argentina. Int J Syst Bacteriol 47:73–77 [CrossRef]
    [Google Scholar]
  14. Itoh T., Yamaguchi T., Zhou P., Takashina T. 2005; Natronolimnobius baerhuensis gen. nov., sp. nov. and Natronolimnobius innermongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia, China. Extremophiles 9111–116 [CrossRef]
    [Google Scholar]
  15. Juez G., Rodriguez-Valera F., Ventosa A., Kushner D. J. 1986; Haloarcula hispanica spec. nov. and Haloferax gibbonsii spec. nov., two new species of extremely halophilic archaebacteria. Syst Appl Microbiol 8:75–79 [CrossRef]
    [Google Scholar]
  16. Kamekura M. 1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria pp  135–161 Edited by Vreeland R. H., Hochstein L. I. Boca Raton, FL: CRC Press;
    [Google Scholar]
  17. Kamekura M., Dyall-Smith M. L. 1995; Taxonomy of the family Halobacteriaceae and the description of two genera Halorubrubacterium and Natrialba . J Gen Appl Microbiol 41:333–350 [CrossRef]
    [Google Scholar]
  18. Kamekura M., Dyall-Smith M. L., Upasani V., Ventosa A., Kates M. 1997; Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum , Natronobacterium magadii , and Natronobacterium pharaonis toHalorubrum , Natrialba , and Natronomonas gen. nov.,respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively. Int J Syst Bacteriol 47:853–857 [CrossRef]
    [Google Scholar]
  19. Kamekura M., Mizuki T., Usami R., Yoshida Y., Horikoshi K., Vreeland R. H. 2004; The potential use of signature bases from 16S rRNA gene sequences to aid the assignment of microbial strains to genera of halobacteria. In Halophilic Microorganisms pp  77–87 Edited by Ventosa A. Heidelberg: Springer;
    [Google Scholar]
  20. Kobayashi T., Kanai H., Hayashi T., Akiba T., Akaboshi R., Horikoshi K. 1992; Haloalkaliphilic maltotriose-forming α -amylase from the archaebacterium Natronococcus sp. strain Ah-6. J Bacteriol 174:3439–3444
    [Google Scholar]
  21. López-Garcia P., Moreira D., López-López A., Rodríguez-Valera F. 2001; A novel haloarchaeal-related lineage is widely distributed in deep oceanic regions. Environ Microbiol 3:72–78 [CrossRef]
    [Google Scholar]
  22. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  23. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  24. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  25. McGenity T. J., Grant W. D. 1995; Transfer of Halobacterium saccharovorum , Halobacterium sodomense , Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb. nov., Halorubrum sodomense comb. nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov.. Syst Appl Microbiol 18237–243 [CrossRef]
    [Google Scholar]
  26. McGenity T. J., Gemmell R. T., Grant W. D. 1998; Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom.nov. and Natrinema pallidum nom. nov. Int J Syst Bacteriol 48:1187–1196 [CrossRef]
    [Google Scholar]
  27. Montalvo-Rodríguez R., Vreeland R. H., Oren A., Kessel M., Betancourt C., López-Garriga J. 1998; Halogeometricum borinquense gen. nov., sp. nov., a novel halophilic archaeon from Puerto Rico. Int J Syst Bacteriol 48:1305–1312 [CrossRef]
    [Google Scholar]
  28. Norton C. F., McGenity T. J., Grant W. D. 1993; Archaeal halophiles (halobacteria) from two British salt mines. J Gen Microbiol 139:1077–1081 [CrossRef]
    [Google Scholar]
  29. Nuttall S. D., Dyall-Smith M. L. 1993; Ch2, a novel halophilic archaeon from an Australian solar saltern. Int J Syst Bacteriol 43:729–734 [CrossRef]
    [Google Scholar]
  30. Oren A. 2000 The order Halobacteriales . In The Prokaryotes : An Evolving Electronic Resource for the Microbiological Community , 3rd edn, release 3·2. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; http://141.150.157.117 : 8080/prokPUB/index.htm
    [Google Scholar]
  31. Oren A., Gurevich P., Gemmell R. T., Teske A. 1995; Halobaculum gomorrense gen. nov., sp. nov. a novel extremely halophilic archaeon from the Dead Sea. Int J Syst Bacteriol 45:747–754 [CrossRef]
    [Google Scholar]
  32. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  33. Oren A., Elevi R., Watanabe S., Ihara K., Corcelli A. 2002; Halomicrobium mukohataei gen. nov., comb. nov. and emended description of Halomicrobium mukohataei . Int J Syst Evol Microbiol 52:1831–1835 [CrossRef]
    [Google Scholar]
  34. Owen R. J., Hill L. R. 1979; The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists , 2nd edn. pp  217–296 Edited by Skinner F. A., Lovelock D. W. London: Academic Press;
    [Google Scholar]
  35. Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1981; Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. Microb Ecol 7:235–243 [CrossRef]
    [Google Scholar]
  36. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  37. Soliman G. S. H., Trüper H. G. 1982; Halobacterium pharaonis sp. nov., a new extremely haloalkaliphilic archaebacterium with low magnesium requirement. Zentralbl Bakteriol Hyg I Abt Orig C 3:318–329
    [Google Scholar]
  38. Tindall B. J. 1992; The family Halobacteriaceae . In The Prokaryotes. A Handbook of Bacteria Ecophysiology , Isolation , Identification , Applications . , 2nd edn. vol 1 pp  768–808 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
  39. Tindall B. J., Ross H. N. M., Grant W. D. 1984; Natronobacterium gen. nov. and Natronococcus gen. nov. two new genera of haloalkaliphilic archaebacteria. Syst Appl Microbiol 5:41–57 [CrossRef]
    [Google Scholar]
  40. Torreblanca M., Rodriguez-Valera F., Juez G., Ventosa A., Kamekura M., Kates M. 1986; Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition and description of Haloarcula gen. nov. and Haloferax gen. nov. Syst Appl Microbiol 8:89–99 [CrossRef]
    [Google Scholar]
  41. Ventosa A., Gutiérrez M. C., Kamekura M., Dyall-Smith M. L. 1999; Proposal to transfer Halococcus turkmenicus , Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49:131–136 [CrossRef]
    [Google Scholar]
  42. Vreeland R. H., Straight S., Krammes J., Dougherty K., Rosenzweig W. D., Kamekura M. 2002; Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles 6:445–452 [CrossRef]
    [Google Scholar]
  43. Wainø M., Tindall B. J., Ingvorsen K. 2000; Halorhabdus utahensis gen nov., sp. nov an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah. Int J Syst Evol Microbiol 50183–190 [CrossRef]
    [Google Scholar]
  44. Wilson K. 1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 2.4.1–2.4.2 Edited by Ausubel F. M., Bent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;
    [Google Scholar]
  45. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase Y. 2000; Natrinema versiforme sp. nov., an extremely halophilic archeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50:1297–1303 [CrossRef]
    [Google Scholar]
  46. Xin H., Itoh T., Zhou P., Suzuki K., Nakase Y. 2001; Natronobacterium nitratireducens sp. nov., a haloalkaliphilic archaeon isolated from a soda lake in China. Int J Syst Evol Microbiol 51:1825–1829 [CrossRef]
    [Google Scholar]
  47. Xu Y., Zhou P., Tian X. 1999; Characterization of two novel haloalkaliphilic archaea Natronorubrum bangense gen.nov., sp. nov. and Natronorubrum tibetense gen. nov., sp. nov. Int J Syst Bacteriol 49:261–266 [CrossRef]
    [Google Scholar]
  48. Xu Y., Wang Z., Xue Y., Zhou P., Ma Y., Ventosa A., Grant W. D. 2001; Natrialba hulunbeirensis sp. nov. and Natrialba chahannaoensis sp. nov., novel haloalkaliphilic archaea from soda lakes in Inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 51:1693–1698 [CrossRef]
    [Google Scholar]
  49. Xue Y., Fan H., Ventosa A., Grant W. D., Jones B. E., Cowan D. A., Ma Y. 2005; Halalkalicoccus tibetensis gen. nov., sp. nov., representing a novel genus of haloalkaliphilic archaea. Int J Syst Evol Microbiol 55:2501–2505 [CrossRef]
    [Google Scholar]
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