1887

Abstract

A Gram-positive, coccus-shaped, lactic acid bacterium, strain LMG 23082, was isolated from steeped maize grains. The organism is homofermentative and produces - and -lactic acid from glucose. 16S rRNA gene sequence analysis revealed that the organism belongs to the genus , with and as nearest neighbours. Genotypic fingerprinting, whole-cell protein electrophoresis, DNA–DNA hybridizations and physiological and biochemical tests allowed differentiation of strain LMG 23082 from other established species. A remarkable feature was that, unlike other pediococci, this bacterium was capable of growth at pH 9·0. The strain studied represents a novel species for which the name sp. nov. is proposed with the type strain LMG 23082 (=BFE 1652=FAIR-E 180=CCUG 51290), the only currently known isolate of the species.

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2006-02-01
2021-04-16
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References

  1. Back W. 1978; Elevation of Pediococcus cerevisiae subsp. dextrinicus Coster and White to species status Pediococcus dextrinicus (Coster and White) comb. nov. Int J Syst Bacteriol 28:523–527 [CrossRef]
    [Google Scholar]
  2. Björkroth J., Korkeala H. 1996; Evaluation of Lactobacillus sake contamination in vacuum-packaged sliced cooked meat products by ribotyping. J Food Prot 59:398–401
    [Google Scholar]
  3. Collins M. D., Williams A. M., Wallbanks S. 1990; The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov. FEMS Microbiol Lett 70:255–262
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. de Man J. C., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135 [CrossRef]
    [Google Scholar]
  6. Dobson C. M., Deneer H., Lee S., Hemmingsen S., Glaze S., Ziola B. 2002; Phylogenetic analysis of the genus Pediococcus , including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer. Int J Syst Evol Microbiol 52:2003–2010 [CrossRef]
    [Google Scholar]
  7. Felis G. E., Torriani S., Dellaglio F. 2005; Reclassification of Pediococcus urinaeequi ( ex Mees 1934) Garvie 1988, as Aerococcus urinaeequi comb. nov. Int J Syst Evol Microbiol 55:1325–1327 [CrossRef]
    [Google Scholar]
  8. Gancheva A., Pot B., Vanhonacker K., Hoste B., Kersters K. 1999; A polyphasic approach towards the identification of strains belonging to Lactobacillus acidophilus and related species. Syst Appl Microbiol 22:573–585 [CrossRef]
    [Google Scholar]
  9. Gevers D., Huys G., Swings J. 2001; Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205:31–36 [CrossRef]
    [Google Scholar]
  10. Hardie J. M., Whiley R. A. 1997; Classification and overview of the genera Streptococcus and Enterococcus . J Appl Microbiol Symp Suppl 83:1S–11S [CrossRef]
    [Google Scholar]
  11. Holzapfel W. H., Franz C. M. A. P., Ludwig W., Back W., Dicks L. M. T. 2005; Genera Pediococcus and Tetragenococcus . In The Prokaryotes 3rd edn., An Evolving Electronic Resource for the Microbiological Community , release 3.15 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; http://link.springer-ny.com/link/service/books/10125/
    [Google Scholar]
  12. Kostinek M., Specht I., Edward V. A., Schillinger U., Hertel C., Holzapfel W. H., Franz C. M. A. P. 2005; Diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of Gari, a traditional African food. Syst Appl Microbiol 28:527–540 [CrossRef]
    [Google Scholar]
  13. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  14. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  15. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics pp  493–521 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  16. Schillinger U., Lücke F.-K. 1987; Identification of lactobacilli from meat and meat products. Food Microbiol 4:199–208 [CrossRef]
    [Google Scholar]
  17. Simpson W. J., Taguchi H. 1995; The genus Pediococcus , with notes on the genera Tetragenococcus and Aerococcus . In The Genera of Lactic Acid Bacteria pp  125–172 Edited by Wood B. J. B., Holzapfel W. H. London: Blackie Academic & Professional;
    [Google Scholar]
  18. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy: the Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  19. Stackebrandt E., Kandler O. 1979; Taxonomy of the genus Cellulomonas , based on phenotypic characters and deoxyribonucleic acid-deoxyribonucleic acid homology and proposal of seven neotype strains. Int J Syst Bacteriol 29:273–282 [CrossRef]
    [Google Scholar]
  20. Thompson F. L., Hoste B., Vandemeulebroecke K., Swings J. 2001; Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24:520–538 [CrossRef]
    [Google Scholar]
  21. Torriani S., Clementi F., Vancanneyt M., Hoste B., Dellaglio F., Kersters K. 2001; Differentiation of Lactobacillus plantarum , L. pentosus and L. paraplantarum species by RAPD-PCR and AFLP. Syst Appl Microbiol 24:554–560 [CrossRef]
    [Google Scholar]
  22. Vancanneyt M., Mengaud J., Cleenwerck I., Hoste B., Dawyndt P., Degivry M. C., Ringuet D., Janssens D., Swings J. 2004; Reclassification of Lactobacillus kefirgranum Takizawa et al . 1994 as Lactobacillus kefiranofaciens subsp. kefirgranum subsp.nov. and emended description of L. kefiranofaciens Fujisawa et al . 1988. Int J Syst Evol Microbiol 54:551–556 [CrossRef]
    [Google Scholar]
  23. Versalovic J., Schneider M., de Bruijn F. J., Lupski J. R. 1994; Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  25. Weiss N. 1991; The genera Pediococcus and Aerococcus . In The Prokaryotes , 2nd edn. vol 2 pp  1502–1507 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K.-H. New York: Springer;
    [Google Scholar]
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