1887

Abstract

A Gram-positive, coccus-shaped, lactic acid bacterium, strain LMG 23082, was isolated from steeped maize grains. The organism is homofermentative and produces - and -lactic acid from glucose. 16S rRNA gene sequence analysis revealed that the organism belongs to the genus , with and as nearest neighbours. Genotypic fingerprinting, whole-cell protein electrophoresis, DNA–DNA hybridizations and physiological and biochemical tests allowed differentiation of strain LMG 23082 from other established species. A remarkable feature was that, unlike other pediococci, this bacterium was capable of growth at pH 9·0. The strain studied represents a novel species for which the name sp. nov. is proposed with the type strain LMG 23082 (=BFE 1652=FAIR-E 180=CCUG 51290), the only currently known isolate of the species.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.63944-0
2006-02-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/2/329.html?itemId=/content/journal/ijsem/10.1099/ijs.0.63944-0&mimeType=html&fmt=ahah

References

  1. Back, W. ( 1978; ). Elevation of Pediococcus cerevisiae subsp. dextrinicus Coster and White to species status Pediococcus dextrinicus (Coster and White) comb. nov. Int J Syst Bacteriol 28, 523–527.[CrossRef]
    [Google Scholar]
  2. Björkroth, J. & Korkeala, H. ( 1996; ). Evaluation of Lactobacillus sake contamination in vacuum-packaged sliced cooked meat products by ribotyping. J Food Prot 59, 398–401.
    [Google Scholar]
  3. Collins, M. D., Williams, A. M. & Wallbanks, S. ( 1990; ). The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov. FEMS Microbiol Lett 70, 255–262.
    [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  5. de Man, J. C., Rogosa, M. & Sharpe, M. E. ( 1960; ). A medium for the cultivation of lactobacilli. J Appl Bacteriol 23, 130–135.[CrossRef]
    [Google Scholar]
  6. Dobson, C. M., Deneer, H., Lee, S., Hemmingsen, S., Glaze, S. & Ziola, B. ( 2002; ). Phylogenetic analysis of the genus Pediococcus, including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer. Int J Syst Evol Microbiol 52, 2003–2010.[CrossRef]
    [Google Scholar]
  7. Felis, G. E., Torriani, S. & Dellaglio, F. ( 2005; ). Reclassification of Pediococcus urinaeequi (ex Mees 1934) Garvie 1988, as Aerococcus urinaeequi comb. nov. Int J Syst Evol Microbiol 55, 1325–1327.[CrossRef]
    [Google Scholar]
  8. Gancheva, A., Pot, B., Vanhonacker, K., Hoste, B. & Kersters, K. ( 1999; ). A polyphasic approach towards the identification of strains belonging to Lactobacillus acidophilus and related species. Syst Appl Microbiol 22, 573–585.[CrossRef]
    [Google Scholar]
  9. Gevers, D., Huys, G. & Swings, J. ( 2001; ). Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205, 31–36.[CrossRef]
    [Google Scholar]
  10. Hardie, J. M. & Whiley, R. A. ( 1997; ). Classification and overview of the genera Streptococcus and Enterococcus. J Appl Microbiol Symp Suppl 83, 1S–11S.[CrossRef]
    [Google Scholar]
  11. Holzapfel, W. H., Franz, C. M. A. P., Ludwig, W., Back, W. & Dicks, L. M. T. ( 2005; ). Genera Pediococcus and Tetragenococcus. In The Prokaryotes, 3rd edn, An Evolving Electronic Resource for the Microbiological Community, release 3.15. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer. http://link.springer-ny.com/link/service/books/10125/
  12. Kostinek, M., Specht, I., Edward, V. A., Schillinger, U., Hertel, C., Holzapfel, W. H. & Franz, C. M. A. P. ( 2005; ). Diversity and technological properties of predominant lactic acid bacteria from fermented cassava used for the preparation of Gari, a traditional African food. Syst Appl Microbiol 28, 527–540.[CrossRef]
    [Google Scholar]
  13. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  14. Pitcher, D. G., Saunders, N. A. & Owen, R. J. ( 1989; ). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef]
    [Google Scholar]
  15. Pot, B., Vandamme, P. & Kersters, K. ( 1994; ). Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by M. Goodfellow & A. G. O'Donnell. Chichester: Wiley.
  16. Schillinger, U. & Lücke, F.-K. ( 1987; ). Identification of lactobacilli from meat and meat products. Food Microbiol 4, 199–208.[CrossRef]
    [Google Scholar]
  17. Simpson, W. J. & Taguchi, H. ( 1995; ). The genus Pediococcus, with notes on the genera Tetragenococcus and Aerococcus. In The Genera of Lactic Acid Bacteria, pp. 125–172. Edited by B. J. B. Wood & W. H. Holzapfel. London: Blackie Academic & Professional.
  18. Sneath, P. H. A. & Sokal, R. R. ( 1973; ). Numerical Taxonomy: the Principles and Practice of Numerical Classification. San Francisco: W. H. Freeman.
  19. Stackebrandt, E. & Kandler, O. ( 1979; ). Taxonomy of the genus Cellulomonas, based on phenotypic characters and deoxyribonucleic acid-deoxyribonucleic acid homology and proposal of seven neotype strains. Int J Syst Bacteriol 29, 273–282.[CrossRef]
    [Google Scholar]
  20. Thompson, F. L., Hoste, B., Vandemeulebroecke, K. & Swings, J. ( 2001; ). Genomic diversity amongst Vibrio isolates from different sources determined by fluorescent amplified fragment length polymorphism. Syst Appl Microbiol 24, 520–538.[CrossRef]
    [Google Scholar]
  21. Torriani, S., Clementi, F., Vancanneyt, M., Hoste, B., Dellaglio, F. & Kersters, K. ( 2001; ). Differentiation of Lactobacillus plantarum, L. pentosus and L. paraplantarum species by RAPD-PCR and AFLP. Syst Appl Microbiol 24, 554–560.[CrossRef]
    [Google Scholar]
  22. Vancanneyt, M., Mengaud, J., Cleenwerck, I., Hoste, B., Dawyndt, P., Degivry, M. C., Ringuet, D., Janssens, D. & Swings, J. ( 2004; ). Reclassification of Lactobacillus kefirgranum Takizawa et al. 1994 as Lactobacillus kefiranofaciens subsp. kefirgranum subsp. nov. and emended description of L. kefiranofaciens Fujisawa et al. 1988. Int J Syst Evol Microbiol 54, 551–556.[CrossRef]
    [Google Scholar]
  23. Versalovic, J., Schneider, M., de Bruijn, F. J. & Lupski, J. R. ( 1994; ). Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5, 25–40.
    [Google Scholar]
  24. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  25. Weiss, N. ( 1991; ). The genera Pediococcus and Aerococcus. In The Prokaryotes, 2nd edn, vol. 2, pp. 1502–1507. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.63944-0
Loading
/content/journal/ijsem/10.1099/ijs.0.63944-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error